In the past decade, light microscopy has evolved to become the leading tool for the structural and functional investigation of biological systems. Rapid advances in the fields of photochemistry and biotechnology have enabled innovative biological research with continuously increasing spatiotemporal resolution and throughput. The versatility of advanced light microscopy and its broad application radius in fundamental research, environmental monitoring and drug discovery make it an indispensible technology. In line with this evolution, the Dept. of Molecular Biotechnology (Ghent University) established in 2009 a facility, dedicated to advanced light microscopy, with the goal of performing high level scientific research in a multidisciplinary context.