Laser microdissection enables immunofluorescent microscopic evaluation and subsequent isolation or manipulation of single cells or homogeneous cell clusters in heterogeneous tissues. This isolation occurs without direct contact minimizing risk of contamination. The collected samples can be used for downstream DNA and RNA sequencing or protein analysis. Therefore it enables researchers to combine information on the spatial localization of cells with the downstream analysis of the expression profile of RNA, DNA or proteins. Moreover, the device allows ablation experiments on life cells followed by life cell imaging.

Specifications:

Inverted Axiovert 200 Zeiss microscope

Objectives: 5X, 10X, 20X, 40X, 63X air and 100X oil

Four colour channels for epifluorescence visualisation (HXP 120)

- DAPI (ex. G 365 /em. BP 445-50)

- FITC (ex. BP 470-40 / em. BP 525-50)

- AF555 (ex. BP 546-12 / em. BP 575-640)

- SpectrumAqua (ex. BP 436-20 / em. BP 480-40)

Two detectors: Axiocam 506 mono and Axiocam 506 color

Pulsed UVa-laser for sample collection

PALM RoboSoftware

ApoTome for optical sectioning (not combined with LCM operation)

Stage top Incubator (temperature control)

RoboMover

Multiple inserts (200 or 500 µl tubes to load load either a single cap, eight caps, up to 12 eight cap strips or a 96 well plate)

Live cell collector