Infrastructure

Roche Digital LightCycler (6-color digital PCR)

Type
Equipment
Acronym
Roche DLC
Code
APP/00059
Date of commissioning
01 March 2024 → …
Research disciplines
  • Medical and health sciences
    • Molecular diagnostics
    • Nucleic acids
Keywords
dPCR PCR-based quantification PCR digital PCR RNA DNA
Brand Name/manufacturer
Digital LightCycler/Roche
Other information
 
Description

The Digital LightCycler® System is a semi-automated system for detection and absolute quantification of nucleic acid targets via digital PCR technology (dPCR). The system is comprised of the Digital LightCycler® Analyzer and the Digital LightCycler® Partitioning Engine.

The main features of the system are:

  • 6 fluorescence channels for multichannel detection
  • 3 analysis types (absolute quantification, CNV, indel)
  • 3 types of nanowell plates (universal, high resolution, high sensitivity) for different applications
  • Partitioning of up to 8 samples on 1 nanowell plate in the partitioning engine
  • Processing of up to 96 samples on 12 nanowell plates on the analyzer