The BioRad CFX Duet can perform real-time Polymerase Chain Reactions (qPCR) with the following specifications :

• Thermal cycler :
  • 96 well plates
  • Max ramp rate = 5 °C/sec
  • Average ramp rate = 3.3 °C/sec
  • Lid = 30-110 °C
• Gradient qPCR
  • operational range = 30 - 100 °C
  • Programmable span = 1 - 24 °C
• Optical detection :
  • Excitation : 3 filtered LEDs
  • Detection : 3 filtered photodiodes
  • Range of excitation/emission : 450 - 580 nm
  • Sensitivity : detects 1 copy of target sequence in human genomic DNA
  • Dynamic range : 10 orders of magnitude
• Scan time :
  • all channels : 12 sec.
  • FAM/SYBR Green only : 3 sec.