Infrastructure

Lionheart™ FX Automated Microscope

Type
Equipment
Acronym
Lionheart
Code
APP/00054
Date of commissioning
23 September 2022 → …
Research disciplines
  • Medical and health sciences
    • Medical biophysics
    • Cell physiology
    • Molecular physiology
    • Organ physiology
    • Pathophysiology
Keywords
Automated imaging Live cell imaging Image analysis Brightfield Fluorescence Widefield Imaging Microscope
Brand Name/manufacturer
BioTek
Other information
 
Description

The lionheart FX is an automated microscope that allows brightfield, color brightfield and fluorescence widefield imaging. The microscope is optimized for automated image acquisition over time and contains an incubator for temperature control. Automated image pre-processing optimizes images for downstream analysis, from cell counting to characterization of subcellular details

Specifications:

  • Sampleholders

o             four 35 mm round dishes

o             multiwell plates

o             2-position slide holder

  • Autofocus: Image based and laser based
  • Environmental control cover: For temperature control to 40 °C and "darkroom" fluorescence, incl. Humidity control chamber
  • Objectives

o             20x Objective, Olympus Plan Apochromat, NA 0.4, WD 1.2

o             2.5x (2.75x eff), Zeiss Plan Fluorite, NA 0.12, WD 2.5

o             High contrast brightfield kit, 4x Objective included

  • Filter cubes & LEDs

o             DAPI filter cube: Ex. 377/50 Em. 447/60 mirror 409+ 365 nm LED cube

o             GFP filter cube: Ex. 469/35 Em. 525/39 mirror 497+ 465 nm LED cub

o             Texas Red filter cube: Ex. 586/15 Em. 647/57 mirror 605+ 590 nm LED cube      

o             CY5 Filter cube: Ex. 628/40 Em. 685/40 mirror 660+ 623nm LED cube

  • Software GEN5IPRIME (Gen5 Image Prime)