Infrastructuur

Lionheart™ FX geautomatiseerde microscoop

Type
Apparatuur
Acroniem
Lionheart
Code
APP/00054
Datum van ingebruikname
23-09-2022 → …
Onderzoeksdisciplines
  • Medical and health sciences
    • Medical biophysics
    • Cell physiology
    • Molecular physiology
    • Organ physiology
    • Pathophysiology
Trefwoorden
Brightfield Beeldanalyse Microscoop Widefield Beeldvorming van levende cellen Fluorescentie Automatische beeldvorming Beeldvorming
Merknaam/fabrikant
BioTek
Andere informatie
 
Omschrijving

The lionheart FX is an automated microscope that allows brightfield, color brightfield and fluorescence widefield imaging. The microscope is optimized for automated image acquisition over time and contains an incubator for temperature control. Automated image pre-processing optimizes images for downstream analysis, from cell counting to characterization of subcellular details

Specifications:

  • Sampleholders

o             four 35 mm round dishes

o             multiwell plates

o             2-position slide holder

  • Autofocus: Image based and laser based
  • Environmental control cover: For temperature control to 40 °C and "darkroom" fluorescence, incl. Humidity control chamber
  • Objectives

o             20x Objective, Olympus Plan Apochromat, NA 0.4, WD 1.2

o             2.5x (2.75x eff), Zeiss Plan Fluorite, NA 0.12, WD 2.5

o             High contrast brightfield kit, 4x Objective included

  • Filter cubes & LEDs

o             DAPI filter cube: Ex. 377/50 Em. 447/60 mirror 409+ 365 nm LED cube

o             GFP filter cube: Ex. 469/35 Em. 525/39 mirror 497+ 465 nm LED cub

o             Texas Red filter cube: Ex. 586/15 Em. 647/57 mirror 605+ 590 nm LED cube      

o             CY5 Filter cube: Ex. 628/40 Em. 685/40 mirror 660+ 623nm LED cube

  • Software GEN5IPRIME (Gen5 Image Prime)