It is designed to meet the need for the highest separation performance, for example in biochemical, organic and inorganic compounds often found in complex samples of ecological and biological origin. Therefore, LC-MS/MS can be applied in a wide variety of sectors, such as biotechnology, environmental monitoring, food processing and the pharmaceutical, pesticide and cosmetics industries. With LC-MS/MS the analytes can be analyzed qualitatively and quantitatively. In the analysis of single or multiple analytes in complex environments, in the analysis of trace materials, in the analysis of impurities and metabolites, as well as in the analysis of additives and pesticides in foods, analysis of biological samples such as blood, urine, tissue can be done with high precision and accuracy are obtained. LC-MS/MS is one of the most sensitive methods for the determination of trace amounts of analytes in the sample, as well as for analyzes of multiple analytes. It provides information about the amount, structure and molecular weight of the components in the sample. From small pharmaceutical compounds to the determination of large proteins: polar ionic, thermally unstable and non-volatile compounds have a wide range of applications.