Infrastructure

Leica Tissue Embedding

Type
Equipment
Acronym
Tissue Embedding
Code
APP/00188
Date of commissioning
02 July 2021 → …
Research disciplines
  • Medical and health sciences
    • Tissue engineering
    • Tissue engineering
    • Tissue engineering
Keywords
leica core histology tissue fixation embedding tissue embedding
Brand Name/manufacturer
Leica
Other information
 
Description

Leica Tissue Embedding is a process in which biological samples (such as tissue) are prepared for microscopic examination. This process involves several steps that help preserve the tissue, allowing it to be analyzed later under a microscope.

The embedding process itself involves placing the tissue into a solid material, such as paraffin or plastic, to stabilize and protect it. Here are the key steps of the process:

  1. Fixation: The tissue is first fixed, usually with a chemical solution like formalin, to preserve the cells and tissues in their natural state.

  2. Dehydration: The tissue is then dried by placing it in solvents (such as alcohol) to remove the water from the tissue.

  3. Clearance: The solvent is removed and replaced with a solution that prepares the tissue for the embedding material (such as paraffin).

  4. Embedding: The tissue is submerged in the embedding material (usually paraffin or a resin) and hardens, so the tissue becomes embedded in the material and is firm enough to be cut into thin sections.

  5. Sectioning: After embedding, the tissue is cut into thin slices using a microtome (a device for cutting thin sections of tissue). These slices are then placed on glass slides and can be stained for microscopic examination.

Leica is a brand that provides equipment and technologies that facilitate the tissue embedding process and subsequent microscopy, for example, by using automated systems that streamline the entire process for research laboratories.