Research Explorer

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Researcher

Yves Briers

Awards & Distinctions

173 Results

2025

A VersaTile approach to reprogram the specificity of the R2-type tailocin towards different serotypes of Escherichia coli and Klebsiella pneumoniae

Dorien DamsCélia PasAgnieszka ŁątkaZuzanna Drulis-KawaLars FieselerYves Briers

A1 Journal Article in ANTIBIOTICS-BASEL

2024

A VersaTile approach to reprogram and broaden the specificity of R2 tailocins towards E. coli and K. pneumoniae

Dorien DamsCélia PasAgnieszka ŁątkaZuzanna Drulis-Kawalars FieselerYves Briers

A comparative guide to expression systems for phage lysin production

Emma CremelieRoberto VázquezYves Briers

A1 Journal Article in ESSAYS IN BIOCHEMISTRY

Accelerating the lysin hit-to-lead pipeline through metagenomic lysin identification, modular engineering and ultrahigh-throughput screening

Lisa DuyvejonckYves Briers

Analyzing designer cellulosomes for their industrial competitiveness

Babette LamoteJulie VanderstraetenNette De RidderKenan MeertWout BoerjanYves Briers

Antibacterial synergy between a phage endolysin and citric acid against the Gram-negative kiwifruit pathogen Pseudomonas syringae pv. actinidiae

Hazel M. SissonRobert D. FagerlundSimon A. JacksonYves BriersSuzanne L. WarringPeter C. Fineran

A1 Journal Article in APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Approaches to the development of designer cellulosomes for biorefinery applications

Babette LamoteYves BriersMarjan De Mey

Colocalization of glycolytic enzymes on a synthetic protein scaffold

Marte EliasKenan MeertJulie VanderstraetenBabette LamoteYves BriersMarjan De Mey

DepoScope : accurate phage depolymerase annotation and domain delineation using large language models

Robby Concha-ElokoMichiel StockBernard De BaetsYves BriersRafael SanjuánPilar Domingo-CalapDimitri Boeckaerts

A1 Journal Article in PLOS COMPUTATIONAL BIOLOGY

Development of a standardized plug-and-play strategy for receptor-binding protein engineering of Klebsiella pneumoniae phages

Dorien DamsAgnieszka ŁątkaMathilde HulsensZuzanna Drulis-KawaYves Briers

Dissecting the unknown specificity of cell wall-binding domains of phage endolysins

Roberto VázquezDiana Gutierrez FernandezZoë De ZutterBjorn CrielYves Briers

Endolysin NC5 improves early cloxacillin treatment in a mouse model of Streptococcus uberis mastitis

Niels Vander ElstJulie BellemansRob LavigneYves BriersEvelyne Meyer

A1 Journal Article in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Harnessing phage tailspike modularity to reprogram phages and phage tail-like bacteriocins towards Shiga toxin-producing Escherichia coli serogroups

Célia PasYves BriersLars Fieseler

Lessons learned from a systematic structural-functional characterization of cell wall binding domains (CBDs) from phage endolysins

Roberto VázquezDiana Gutierrez FernandezZoë de ZutterBjorn CrielYves Briers

Meeting report of the second symposium of the Belgian Society for Viruses of Microbes and launch of the Phage Valley

Salome DesmechtAgnieszka ŁątkaPieter-Jan CeyssensAbel Garcia-PinoAnnika GillisRob LavigneGipsi Lima-MendezJelle MatthijnssensRoberto VázquezJolien Venneman et al.

A1 Journal Article in VIRUSES-BASEL

Metagenomic exploration of phage-derived lysins for precise antibacterial action

Iris PottieLisa DuyvejonckRoberto VázquezYves Briers

Phage lysins for intestinal microbiome modulation : current challenges and enabling techniques

Iris PottieRoberto VázquezTom Van de WieleYves Briers

A1 Journal Article in GUT MICROBES

Prediction of Klebsiella phage-host specificity at the strain level

Dimitri BoeckaertsMichiel StockCelia Ferriol-GonzálezJesús Oteo-IglesiasRafael SanjuánPilar Domingo-CalapBernard De BaetsYves Briers

A1 Journal Article in NATURE COMMUNICATIONS

The new SH3b_T domain increases the structural and functional variability among SH3b-like CBDs from staphylococcal phage endolysins

Roberto VázquezDiana Gutierrez FernandezDennis GrimonLucía FernándezPilar GarcíaAna RodríguezYves Briers

A1 Journal Article in PROBIOTICS AND ANTIMICROBIAL PROTEINS

You get what you test for : the killing effect of phage lysins is highly dependent on buffer tonicity and ionic strength

Roberto VázquezDiana Gutierrez FernandezZoë DezutterBjorn CrielPhilippe De GrooteYves Briers

A1 Journal Article in MICROBIAL BIOTECHNOLOGY

2023

Biology-based machine learning to predict phage-host specificity for the clinical phage therapist

Dimitri BoeckaertsCélia PasAgnieszka ŁątkaMichiel StockCelia Ferriol-GonzálezRafael SanjuanPilar Domingo-CalapLars FieselerBernard De BaetsYves Briers

Breaking down walls : towards a functional-based metagenomic discovery platform for phage-derived lysins

Iris PottieLisa DuyvejonckRoberto VázquezYves Briers

CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers

Hugo OliveiraSilvio SantosDiana P. PiresDimitri BoeckaertsGraca PintoRita DominguesJennifer OteroYves BriersRob LavigneMathias Schmelcher et al.

A1 Journal Article in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Current challenges in designer cellulosome engineering

Babette LamoteMaria João de Carvalho Maurício da FonsecaJulie VanderstraetenKenan MeertMarte EliasYves Briers

A1 Journal Article in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Development of engineered endolysins with in vitro intracellular activity against streptococcal bovine mastitis‐causing pathogens

Niels Vander ElstJoni BertHerman FavoreelRob LavigneEvelyne MeyerYves Briers

A1 Journal Article in MICROBIAL BIOTECHNOLOGY

Dismantling superbugs : engineering third-generation lysins as enzybiotics for burn wound infections

Vincent De MaesschalckRob LavigneYves BriersJan PaeshuyseDiana Gutierrez Fernandez

Distinct mode of action of a highly stable, engineered phage lysin killing Gram-negative bacteria

Hans GerstmansLisa DuyvejonckRoberto VázquezInes StaesJimmy BorlooKarim Abdelkader Soufi AbdelkaderJeroen LeroyEmma CremelieDiana Gutierrez FernandezHéctor Tamés-Caunedo et al.

A1 Journal Article in MICROBIOLOGY SPECTRUM

Endolysin NC5 improves early cloxacillin treatment in a mouse model for bovine Streptococcus uberis mastitis

Julie BellemansNiels Vander ElstRob LavigneYves BriersEvelyne Meyer

Engineering of bacteriophage-derived endolysins to treat streptococcal mammary gland infections

Niels Vander ElstEvelyne MeyerYves BriersRob Lavigne

Foundation of the Belgian Society for Viruses of Microbes and meeting report of its inaugural symposium

Agnieszka ŁątkaAbram AertsenDimitri BoeckaertsBob BlasdelPieter-Jan CeyssensAbel Garcia-PinoAnnika GillisRob LavigneGipsi Lima-MendezJelle Matthijnssens et al.

A1 Journal Article in VIRUSES-BASEL

Integration of high-throughput specificity profiling and engineering of xylan-active enzymes and xylanosomes

Maria João de Carvalho Maurício da FonsecaYves BriersIngeborg StalsTom DesmetAnita Van Landschoot

Phage lysins as a promising alternative class of antibiotics : a metagenomics-driven high-throughput platform for the discovery of novel lysins

Lisa DuyvejonckIris PottieRoberto VázquezWannes Van WassenberghYves Briers

Phage tailspike modularity and horizontal gene transfer reveals specificity towards E. coli O-antigen serogroups

Célia PasAgnieszka ŁątkaLars FieselerYves Briers

A1 Journal Article in VIROLOGY JOURNAL

Phages and engineered lysins as an effective tool to combat Gram-negative foodborne pathogens

Aleksandra Maria KocotYves BriersMagdalena Plotka

A1 Journal Article in COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY

Predicting and understanding phage-host interactions with machine learning tools based on phage receptor-binding proteins

Dimitri BoeckaertsYves BriersBernard De BaetsMichiel Stock

Structural and functional diversity of cell wall binding domains in staphylococcal endolysins

Roberto VázquezDiana Gutierrez FernandezBjorn CrielAna RodríguezPilar GarcíaYves Briers

Structural and functional diversity of staphylococcal cell wall binding domains

Roberto VázquezDiana Gutierrez FernandezZoë DezutterBjorn CrielYves Briers

Understanding the RBP modules function of KP32 as a podophage model

Agnieszka ŁątkaDorien DamsFlavia SqueqliaLennert ScholiersAleksandra OtwinowskaSebastian OlejniczakBarbara MaciejewskaDiana Gutierrez FernandezRita BerisioZuzanna Drulis-Kawa et al.

Understanding the RBP modules function of KP32 as a podophage model

Agnieszka ŁątkaDorien DamsFlavia SqueqliaLennert ScholiersAleksandra OtwinowskaSebastian OlejniczakBarbara MaciejewskaDiana Gutierrez FernandezRita BerisioZuzanna Drulis-Kawa et al.

What's in a name? An overview of the proliferating nomenclature in the field of phage lysins

Roberto VázquezYves Briers

A1 Journal Article in CELLS

2022

A bioluminescence-based ex vivo burn wound model for real-time assessment of novel phage-inspired enzybiotics

Vincent De MaesschalckDiana Gutierrez FernandezJan PaeshuyseYves BriersGreetje Vande VeldeRob Lavigne

A1 Journal Article in PHARMACEUTICS

A blueprint of tail fiber modularity and its relationship with host specificity for STEC serovars

Célia PasAgnieszka ŁątkaLars FieselerYves Briers

A method for the purification of non-pyrogenic bacteriophage derived endolysins with an in vitro or in vivo application

Niels Vander ElstVincent De MaesschalckPhilippe De GrooteEvelyne MeyerRob LavigneYves Briers

Bacteriophage-derived proteins as antimicrobials

Karim Abdelkader Soufi AbdelkaderAmal SaafanYves BriersTarek DishishaYasser GaberAhmed Khairalla

Characterization of endolysins PlySs2 & -9 with in vitro lytic activity against bovine mastitis Streptococcus uberis

Niels Vander ElstSara B. LindenEvelyne MeyerRob LavigneYves BriersDaniel C. Nelson

Combinatorial assembly and optimisation of designer cellulosomes : a galactomannan case study

Julie VanderstraetenMaria João de Carvalho Maurício da FonsecaPhilippe De GrooteDennis GrimonHans GerstmansAmaranta KahnSarah MoraïsEdward A. BayerYves Briers

A1 Journal Article in BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS

Conversion of the free Cellvibrio japonicus xyloglucan degradation system to the cellulosomal mode

Julie VanderstraetenBabette LamoteMaria João de Carvalho Maurício da FonsecaPhilippe De GrooteYves Briers

A1 Journal Article in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Development of a bioluminescent ex vivo wound model to characterize novel phage-based enzybiotics

Vincent De MaesschalckYves BriersGreetje Vande VeldeRob Lavigne

Development of a synthetic, VersaTile strategy for receptor-binding protein engineering of Klebsiella pneumoniae phages

Dorien DamsAgnieszka ŁątkaMathilde HulsensZuzanna Drulis-KawaYves Briers

Development of a synthetic, VersaTile strategy for receptor-binding protein engineering of Klebsiella pneumoniae phages

Dorien DamsAgnieszka ŁątkaMathilde HulsensZuzanna Drulis-KawaYves Briers

Digital phagograms : predicting phage infectivity through a multilayer machine learning approach

Cédric LoodDimitri BoeckaertsMichiel StockBernard De BaetsRob LavigneVera van NoortYves Briers

A1 Journal Article in CURRENT OPINION IN VIROLOGY

Engineering a lysin with intrinsic antibacterial activity (LysMK34) by cecropin a fusion enhances its antibacterial properties against acinetobacter baumannii

Karim Abdelkader Soufi AbdelkaderDiana Gutierrez FernandezHéctor Tamés-CaunedoPatricia Ruas-MadiedoAmal SafaanAhmed S. KhairallaYasser GaberTarek DishishaYves Briers

A1 Journal Article in APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Enzyme colocalisation on synthetic protein scaffolds : a VersaTile approach

Julie VanderstraetenYves BriersTom Desmet

Exploring PhaLP, an open source portal for Phage Lytic Proteins, to engineer modular enzybiotics against vancomycin-resistant Enterococcus faecalis

Bjorn CrielWim Van CriekingeYves Briers

Exploring PhaLP, an open source portal for Phage Lytic Proteins, to engineer modular enzybiotics against vancomycin-resistant Enterococcus faecalis

Bjorn CrielWim Van CriekingeYves Briers

Hacking yeast cells with phage lysins to win the fight against antibiotic resistant bacteria

Emma CremeliePhilippe De GrooteBabette LamoteYves Briers

High-resolution reconstruction of a Jumbo-bacteriophage infecting capsulated bacteria using hyperbranched tail fibers

Ruochen OuyangAna Rita CostaC. Keith CassidyAleksandra OtwinowskaVera C. J. WilliamsAgnieszka ŁątkaPhill J. StansfeldZuzanna Drulis-KawaYves BriersDaniel M. Pelt et al.

A1 Journal Article in NATURE COMMUNICATIONS

Identification of phage receptor-binding protein sequences with hidden Markov models and an extreme gradient boosting classifier

Dimitri BoeckaertsMichiel StockBernard De BaetsYves Briers

A1 Journal Article in VIRUSES-BASEL

Insight into key structural points of capsule-targeting phage depolymerases specific to Klebsiella pneumoniae K63

Aleksandra OtwinowskaSebastian OlejniczakBarbara MaciejewskaAgnieszka ŁątkaFlavia SquegliaYves BriersTomasz KościółekRafał MostowyRita BerisioZuzanna Drulis-Kawa

Launch of the Belgian Society for Viruses of Microbes

Abram AerstenBob BlasdelDimitri BoeckaertsPieter-Jan CeyssensAbel Garcia-PinoAnnika GillisAgnieszka ŁątkaRob LavigneGipsi Lima-MendezJelle Matthijnssens et al.

Machine learning-guided engineering of modular enzybiotics to target vancomycin-resistant Enterococcus faecalis infections of the urinary tract

Bjorn CrielWim Van CriekingeYves Briers

Phages in feces : developing a platform for discovery of Enterococcus lysins

Iris PottieLisa DuyvejonckYves Briers

Rapid and high-throughput evaluation of diverse configura-tions of engineered lysins using the VersaTile technique

Lisa DuyvejonckHans GerstmansMichiel StockDennis GrimonRob LavigneYves Briers

SEVAtile : a standardised DNA assembly method optimised for Pseudomonas

Eveline‐Marie LammensMaarten BoonDennis GrimonYves BriersRob Lavigne

A1 Journal Article in MICROBIAL BIOTECHNOLOGY

Scaffolding for product yield maximization of fine chemicals in microbial cell factories

Marte EliasBabette LamoteJulie VanderstraetenBrecht De PaepeMarjan De MeyYves Briers

Scaffolding for product yield maximization of fine chemicals in microbial cell factories

Marte EliasBabette LamoteJulie VanderstraetenBrecht De PaepeMarjan De MeyYves Briers

Synthetic phage tail-like bacteriocins targeting Shiga toxin-producing Escherichia coli

Célia PasDorien DamsAgnieszka ŁątkaLars FieselerYves Briers

The organization and evolution of receptor-binding proteins in Klebsiella phages by exploiting novel tools in synthetic biology

Agnieszka ŁątkaDorien DamsFlavia SqueqliaLennert ScholiersMathilde HulsensAleksandra OtwinowskaSebastian OlejniczakBarbara MaciejewskaRita BerisioZuzanna Drulis-Kawa et al.

The specific capsule depolymerase of phage PMK34 sensitizes Acinetobacter baumannii to serum killing

Karim Abdelkader Soufi AbdelkaderDiana Gutierrez FernandezAgnieszka ŁątkaDimitri BoeckaertsZuzanna Drulis-KawaBjorn CrielHans GerstmansAmal SafaanAhmed S. KhairallaYasser Gaber et al.

A1 Journal Article in ANTIBIOTICS-BASEL

Towards a high-throughput lysin discovery platform based on functional metagenomics

Lisa DuyvejonckIris PottieYves Briers

Towards industrial competitiveness of designer cellulosomes using the VersaTile technique

Babette LamoteEmma CremelieJulie VanderstraetenMaria João de Carvalho Maurício da FonsecaMarjan De MeyYves Briers

Towards industrial competitiveness of designer cellulosomes using the VersaTile technique

Babette LamoteEmma CremelieJulie VanderstraetenMaria João de Carvalho Maurício da FonsecaMarjan De MeyYves Briers

Transcriptional organization of the Salmonella Typhimurium phage P22 pid ORFan locus

Sanne WolputAngela MakumiLaura WickeLeonard E. BaeckerWilliam CenensYves BriersNicolas A. WennerSian V. OwenJay C. D. HintonRob Lavigne et al.

A1 Journal Article in INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Two novel approaches to identify phage receptor-binding protein sequences

Dimitri BoeckaertsMichiel StockBernard De BaetsYves Briers

Unravelling the function of carbohydrate binding domains in a highly specific tailspike of Klebsiella phage KP32

Agnieszka ŁątkaFlavia SqueqliaDorien DamsLennert ScholiersAleksandra OtwinowskaSebastian OlejniczakDiana Gutierrez FernandezBarbara MaciejewskaRita BerisioZuzanna Drulis-Kawa et al.

Unravelling the function of the carbohydrate binding domains in a highly specific tailspike of Klebsiella phage KP32

Agnieszka ŁątkaFlavia SqueqliaDorien DamsLennert ScholiersAleksandra OtwinowskaSebastian OlejniczakDiana Gutierrez FernandezBarbara MaciejewskaRita BerisioZuzanna Drulis-Kawa et al.

2021

CkP1 is a novel S16-like broad-host-range myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibres

Hugo OliveiraSilvio B. SantosDiana P. PiresGraca PintoRita DominguesYves BriersMathias SchmelcherAndreas DötschJoana Azeredo

Crowdsourced mapping of unexplored target space of kinase inhibitors

Anna CichonskaBalaguru RavikumarRobert J. AllawayFangping WanSungjoon ParkOlexandr IsayevShuya LiMichael MasonAndrew LambZiaurrehman Tanoli et al.

A1 Journal Article in NATURE COMMUNICATIONS

Design and selection of engineered lytic proteins with Staphylococcus aureus decolonizing activity

Diana Gutierrez FernandezLorena Rodríguez-RubioPatricia Ruas-MadiedoLucía FernándezAna Belén CampeloYves BriersMartin Weiss NielsenKarl PedersenRob LavignePilar García et al.

A1 Journal Article in FRONTIERS IN MICROBIOLOGY

Developing Innolysins against Campylobacter jejuni using a novel prophage receptor-binding protein

Athina ZamparaMartine C. Holst SørensenYilmaz Emre GencayDennis GrimonSebastian Hougaard KristiansenLallana Skaarup JørgensenJosephine Rejgaard KristensenYves BriersAnne Elsser-GravesenLone Brøndsted

A1 Journal Article in FRONTIERS IN MICROBIOLOGY

Development of a bioluminescent ex vivo wound model to characterize novel phage-based enzybiotics

Vincent De MaesschalckGreetje Vande VeldeYves BriersRob Lavigne

Engineering the modular receptor-binding proteins of Klebsiella phages switches their capsule serotype specificity

Agnieszka ŁątkaSebastien LemireDennis GrimonDorien DamsBarbara MaciejewskaTimothy LuZuzanna Drulis-KawaYves Briers

A1 Journal Article in MBIO

Lysins breaking down the walls of Gram-negative bacteria, no longer a no-go

Diana Gutierrez FernandezYves Briers

A1 Journal Article in CURRENT OPINION IN BIOTECHNOLOGY

PhaLP : a database for the study of phage lytic proteins and their evolution

Bjorn CrielSteff TaelmanWim Van CriekingeMichiel StockYves Briers

A1 Journal Article in VIRUSES-BASEL

Predicting bacteriophage hosts based on sequences of annotated receptor-binding proteins

Dimitri BoeckaertsMichiel StockBjorn CrielHans GerstmansBernard De BaetsYves Briers

A1 Journal Article in SCIENTIFIC REPORTS

Rapid and high-throughput evaluation of diverse configurations of engineered lysins using the VersaTile technique

Lisa DuyvejonckHans GerstmansMichiel StockDennis GrimonRob LavigneYves Briers

A1 Journal Article in ANTIBIOTICS-BASEL

Synergistic action of phage phiIPLA-RODI and lytic protein CHAPSH3b : a combination strategy to target Staphylococcus aureus biofilms

Ana Catarina DuarteLucía FernándezVincent De MaesschalckDiana Gutierrez FernandezAna Belén CampeloYves BriersRob LavigneAna RodríguezPilar García

A1 Journal Article in NPJ BIOFILMS AND MICROBIOMES

2020

A VersaTile driven platform for rapid hit-to-lead development of engineered lysins

Hans GerstmansDennis GrimonDiana Gutierrez FernandezCédric LoodAna RodríguezVera van NoortJeroen LammertynRob LavigneYves Briers

A1 Journal Article in SCIENCE ADVANCES

Advanced engineering of third-generation lysins and formulation strategies for clinical applications

Vincent De MaesschalckDiana Gutierrez FernandezJan PaeshuyseRob LavigneYves Briers

A1 Journal Article in CRITICAL REVIEWS IN MICROBIOLOGY

Characterization of the bacteriophage-derived endolysins PlySs2 and PlySs9 with in vitro lytic activity against bovine mastitis streptococcus uberis

Niels Vander ElstSara B. LindenRob LavigneEvelyne MeyerYves BriersDaniel C. Nelson

A1 Journal Article in ANTIBIOTICS-BASEL

Development of the VersaTile technique and a microdroplet assay for the ultrahigh-throughput engineering of lysins as antibacterial agents

Hans GerstmansYves BriersJeroen LammertynRob Lavigne

Developments and opportunities of bacteriophage lytic proteins for therapeutics against gram-negative pathogens

Diana Gutierrez FernandezYves Briers

Bookchapter in Bacterial viruses : exploitation for biocontrol and therapeutics

Exploiting phage receptor binding proteins to enable endolysins to kill Gram-negative bacteria

Athina ZamparaMartine C. Holst SørensenDennis GrimonFabio AntenucciAmira Ruslanovna VittValeria BortolaiaYves BriersLone Brøndsted

A1 Journal Article in SCIENTIFIC REPORTS

High-throughput generation of product profiles for arabinoxylan-active enzymes from metagenomes

Maria João de Carvalho Maurício da FonsecaZachary ArmstrongStephen G. WithersYves Briers

A1 Journal Article in APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Integrative omics analysis of Pseudomonas aeruginosa virus PA5oct highlights the molecular complexity of jumbo phages

Cédric LoodKatarzyna Danis‐WlodarczykBob G. BlasdelHo Bin JangDieter VandenheuvelYves BriersJean‐Paul NobenVera NoortZuzanna Drulis‐KawaRob Lavigne

A1 Journal Article in ENVIRONMENTAL MICROBIOLOGY

Lysin LysMK34 of Acinetobacter baumannii bacteriophage PMK34 has a turgor pressure-dependent intrinsic antibacterial activity and reverts colistin resistance

Karim Abdelkader Soufi AbdelkaderDiana Gutierrez FernandezDennis GrimonPatricia Ruas-MadiedoCédric LoodRob LavigneAmal SafaanAhmed S. KhairallaYasser GaberTarek Dishisha et al.

A1 Journal Article in APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Phage lysins as simple as Lego

Quality control and statistical evaluation of combinatorial DNA libraries using nanopore sequencing

Cédric LoodHans GerstmansYves BriersVera van NoortRob Lavigne

A1 Journal Article in BIOTECHNIQUES

Structural and functional studies of a klebsiella phage capsule depolymerase tailspike : mechanistic insights into capsular degradation

Flavia SquegliaBarbara MaciejewskaAgnieszka ŁątkaAlessia RuggieroYves BriersZuzanna Drulis-KawaRita Berisio

A1 Journal Article in STRUCTURE

Synthetic protein scaffolds for the colocalisation of co-acting enzymes

Julie VanderstraetenYves Briers

A1 Journal Article in BIOTECHNOLOGY ADVANCES

Two distinct modes of lysis regulation in Campylobacter Fletchervirus and Firehammervirus phages

Athina ZamparaStephen J. AhernYves BriersLone BrøndstedMartine Camilla Holst Sørensen

A1 Journal Article in VIRUSES-BASEL

2019

Characterization and in vitro engineering of exopolysaccharide (EPS) depolymerases originated from Klebsiella phages

Agnieszka ŁątkaZuzanna Drulis-KawaYves Briers

Engineering of receptor-binding proteins in bacteriophages and phage tail-like bacteriocins

Dorien DamsLone BrøndstedZuzanna Drulis-KawaYves Briers

A1 Journal Article in BIOCHEMICAL SOCIETY TRANSACTIONS

Enzybiotics : enzyme-based antibacterials as therapeutics

Dorien DamsYves Briers

Bookchapter in Therapeutic enzymes : function and clinical implications

High-throughput substrate specificity analysis of metagenomic-derived arabinoxylan-active enzymes

Maria João de Carvalho Maurício da FonsecaZachary ArmstrongStephen G WithersYves Briers

Modeling the architecture of depolymerase-containing receptor binding proteins in Klebsiella phages

Agnieszka ŁątkaPetr G LeimanZuzanna Drulis-KawaYves Briers

A1 Journal Article in FRONTIERS IN MICROBIOLOGY

Modelling the architecture of depolymerase-containing receptor binding proteins in Klebsiella phages

Agnieszka ŁątkaPetr G LeimanZuzanna Drulis-KawaYves Briers

Phage lytic enzymes

Editorial material

The preclinical and clinical progress of bacteriophages and their lytic enzymes : the parts are easier than the whole

Karim Abdelkader Soufi AbdelkaderHans GerstmansAmal SaafanTarek DishishaYves Briers

A1 Journal Article in VIRUSES-BASEL

VersaTile : a high throughput DNA assembly method for the rapid construction and evaluation of cellulosome components

Julie VanderstraetenMaria João de Carvalho Maurício da FonsecaYves Briers

VersaTile Engineering of lysins: one drop to kill

Hans GerstmansFabrice GielenLorenz Lorenz Van HileghemRob LavigneFlorian HollfelderJeroen LammertynYves Briers

VersaTile Platform to create engineered proteins active against the main pathogens implicated in burn wounds

Diana Gutierrez FernandezHans GerstmansDennis GrimonPilar GarciaAna RodriguezRob LavigneYves Briers

2018

Analysis of the substrate specificity of α-L-arabinofuranosidases by DNA sequencer-aided fluorophore-assisted carbohydrate electrophoresis

Maria João de Carvalho Maurício da FonsecaEdita JurakKim KatajaEmma R MasterJean-Guy BerrinIngeborg StalsTom DesmetAnita Van LandschootYves Briers

A1 Journal Article in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Designer cellulosomics for a customized conversion of lignocellulosic biomass

Julie VanderstraetenMaria João de Carvalho Maurício da FonsecaArno ThibauFiona HoogheYves Briers

Designer cellulosomics for a customized conversion of lignocellulosic biomass to valuable bulk and fine chemicals

Julie VanderstraetenMaria João de Carvalho Maurício da FonsecaArno ThibauF HoogheYves Briers

Enzybiotics as a crop protection product against the kiwi pathogen Pseudomonas syringae pv actinidiae

Dorien DamsHans GerstmansTom Van EeckhoutFilip DebersaquesDanny VereeckeYves Briers

High-throughput design and analysis of modular endolysins to develop lead candidates using VersaTile Shuffling

Hans GerstmansDennis GrimonCédric LoodTom CoenyeRob LavigneYves Briers

Inhibitory and bactericidal effect of Artilysin® Art-175 against colistin-resistant mcr-1-positive Escherichia coli isolates

Eva SchirmeierPia ZimmermannVeronika HofmannManfred BieblHans GerstmansVeerle ET MaervoetYves Briers

A1 Journal Article in INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS

Iterative chemical engineering of vancomycin leads to novel vancomycin analogs with a high in vitro therapeutic index

Nigam M MishraIzabela StolarzewiczDavid CannaertsJoris SchuermansRob LavigneYannick LoozBart LanduytLiliane SchoofsDominique ScholsJan Paeshuyse et al.

A1 Journal Article in FRONTIERS IN MICROBIOLOGY

On the way to create designer xylanosomes

Maria João de Carvalho Maurício da FonsecaJulie VanderstraetenSilke VlyminckSam DoomsYves Briers

Phage-borne depolymerases decrease Klebsiella pneumoniae resistance to innate defense mechanisms

Grazyna Majkowska-SkrobekAgnieszka ŁątkaRita BerisioFlavia SquegliaBarbara MaciejewskaYves BriersZuzanna Drulis-Kawa

A1 Journal Article in FRONTIERS IN MICROBIOLOGY

Synthetic biology of modular endolysins

Hans GerstmansBjorn CrielYves Briers

A1 Journal Article in BIOTECHNOLOGY ADVANCES

Talk : high throughput generation of designer enzybiotics against Acinetobacter baumannii

Hans GerstmansRob LavigneYves Briers

Unlocking the full potential of the VersaTile Platform : an ultra-high-throughput microfluidics-based screening workflow for engineered endolysins

Hans GerstmansFabrice GielenLorenz Van HileghemRob LavigneFlorian HollfelderJeroen LammertynYves Briers

2017

Bacteriophage-encoded virion-associated enzymes to overcome the carbohydrate barriers during the infection process

Agnieszka ŁątkaBarbara MaciejewskaGrazyna Majkowska-SkrobekYves BriersZuzanna Drulis-Kawa

A1 Journal Article in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Characterisation and genome sequence of the lytic Acinetobacter baumannii bacteriophage vB_AbaS_Loki

Dann TurnerMatthew E WandYves BriersRob LavigneJ Mark SuttonDarren M Reynolds

A1 Journal Article in PLOS ONE

High-throughput customized production of arabinoxylan-oligosaccharides (AXOS)

Maria João de Carvalho Maurício da FonsecaIngeborg StalsTom DesmetAnita Van LandschootYves Briers

Klebsiella phages representing a novel clade of viruses with an unknown DNA modification and biotechnologically interesting enzymes

Barbara MaciejewskaBartosz RoszniowskiAkbar EspaillatAgata Kęsik-SzelochGrazyna Majkowska-SkrobekAndrew M KropinskiYves BriersFelipe CavaRob LavigneZuzanna Drulis-Kawa

A1 Journal Article in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Synthetic biology of modular proteins

Veerle MaervoetYves Briers

A1 Journal Article in BIOENGINEERED

The temperate Burkholderia phage AP3 of the Peduovirinae shows efficient antimicrobial activity against B. cenocepacia of the IIIA lineage

Bartosz RoszniowskiAgnieszka ŁątkaBarbara MaciejewskaDieter VandenheuvelTomasz OlszakYves BriersGiles S HoltMiguel A ValvanoRob LavigneDarren L Smith et al.

A1 Journal Article in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

2016

A proposed integrated approach for the preclinical evaluation of phage therapy in Pseudomonas infections

Katarzyna Danis-WlodarczykDieter VandenheuvelHo Bing JangYves BriersTomasz OlszakMichal ArabskiSlawomir WasikMarcin DrabikGerard HigginsJean Tyrrell et al.

A1 Journal Article in SCIENTIFIC REPORTS

Characterization and genome sequencing of a Citrobacter freundii phage CfP1 harboring a lysin active against multidrug-resistant isolates

Hugo OliveiraGraça PintoAna OliveiraCarla OliveiraMaria Alberta FaustinoYves BriersLucília DominguesJoana Azeredo

A1 Journal Article in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Characterization of novel bacteriophages for biocontrol of bacterial blight in leek caused by Pseudomonas syringae pv. porri

Sofie RomboutsAnneleen VolckaertSofie VennemanBart DeclercqDieter VandenheuvelCamille N AllonsiusCinzia Van MalderghemHo B JangYves BriersJean P Noben et al.

A1 Journal Article in FRONTIERS IN MICROBIOLOGY

DUF3380 domain from a Salmonella phage endolysin shows potent N-acetylmuramidase activity

Lorena Rodríguez-RubioHans GerstmansSimon ThorpeStéphane MesnageRob LavigneYves Briers

A1 Journal Article in APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Domain of Unknown Function 3380 (DUF3380) confers potent N-acetylmuramidase activity to a Salmonella phage endolysin

Lorena Rodríguez-RubioHans GerstmansSimon ThorpeStéphane MesnageRob LavigneYves Briers

Efficacy of Artilysin Art-175 against resistant and persistent Acinetobacter baumannii

Valerie DefraineJoris SchuermansBarbara GrymonprezSander K GoversAbram AertsenMaarten FauvartJan MichielsRob LavigneYves Briers

A1 Journal Article in ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

From endolysins to Artilysin®s: novel enzyme-based approaches to kill drug-resistant bacteria

Hans GerstmansLorena Rodríguez-RubioRob LavigneYves Briers

A1 Journal Article in BIOCHEMICAL SOCIETY TRANSACTIONS

The absence of a mature cell wall sacculus in stable Listeria monocytogenes L-form cells is independent of peptidoglycan synthesis

Patrick StuderMarina BorisovaAlexander SchneiderJuan A AyalaChristoph MayerMarkus SchupplerMartin J LoessnerYves Briers

A1 Journal Article in PLOS ONE

Using DSA-FACE to reveal the substrate specificity of α-L-arabinofuranosidases

Maria João de Carvalho Maurício da FonsecaTom DesmetAnita Van LandschootIngeborg StalsYves Briers

‘Artilysation’ of endolysin λSa2lys strongly improves its enzymatic and antibacterial activity against streptococci

Lorena Rodríguez-RubioWai-Ling ChangDiana GutiérrezRob LavigneBeatriz MartínezAna RodríguezSander K GoversAbram AertsenChristine HirlManfred Biebl et al.

A1 Journal Article in SCIENTIFIC REPORTS

2015

Breaking barriers : expansion of the use of endolysins as novel antibacterials against Gram-negative bacteria

Yves BriersRob Lavigne

A1 Journal Article in FUTURE MICROBIOLOGY

Characterization of the newly isolated lytic bacteriophages KTN6 and KT28 and their efficacy against Pseudomonas aeruginosa biofilm

Katarzyna Danis-WlodarczykTomasz OlszakMichal ArabskiSlawomir WasikGrazyna Majkowska-SkrobekDaria AugustyniakGrzegorz GulaYves BriersHo Bin JangDieter Vandenheuvel et al.

A1 Journal Article in PLOS ONE

Correction: Characterization of the newly isolated lytic bacteriophages KTN6 and KT28 and their efficacy against Pseudomonas aeruginosa biofilm

Katarzyna Danis-WlodarczykTomasz OlszakMichal ArabskiSlawomir WasikGrazyna Majkowska-SkrobekDaria AugustyniakGrzegorz GulaYves BriersHo Bin JangDieter Vandenheuvel et al.

Evaluation of the antibacterial and antibiofilm activities of novel CRAMP-vancomycin conjugates with diverse linkers

Nigam M MishraYves BriersChris LamberigtsHans SteenackersStijn RobijnsBart LanduytJos VanderleydenLiliane SchoofsRob LavigneWalter Luyten et al.

A1 Journal Article in ORGANIC & BIOMOLECULAR CHEMISTRY

Metabolite profiling and peptidoglycan analysis of transient cell wall-deficient bacteria in a new Escherichia coli model system

Alexander CambréMichael ZimmermannUwe SauerBram VivijsWilliam CenensChris W MichielsAbram AertsenMartin J LoessnerJean-Paul NobenJuan A Ayala et al.

A1 Journal Article in ENVIRONMENTAL MICROBIOLOGY

Role of the pre-neck appendage protein (Dpo7) from phage vB_SepiS-philPLA7 as an anti-biofilm agent in Staphylococcal species

Diana GutiérrezYves BriersLorena Rodríguez-RubioBeatriz MartínezAna RodríguezRob LavignePilar García

A1 Journal Article in FRONTIERS IN MICROBIOLOGY

The zeamine antibiotics affect the integrity of bacterial membranes

Joleen MasscheleinCharlien ClauwersKaren StalmansKoen NuytsWim De BorggraeveYves BriersAbram AertsenChris W MichielsRob Lavigne

A1 Journal Article in APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Two complete and one draft genome sequence of nonproteolytic Clostridium botulinum type E strains NCTC 8266, NCTC 8550 and NCTC 11219

Charlien ClauwersYves BriersRob LavigneChris W Michiels

A4 Journal Article in GENOME ANNOUNCEMENTS

2014

Art-175 is a highly efficient antibacterial against multidrug-resistant strains and persisters of Pseudomonas aeruginosa

Yves BriersMaarten WalmaghBarbara GrymonprezManfred BieblJean-Paul PirnayValerie DefraineJan MichielsWilliam CenensAbram AertsenStefan Miller et al.

A1 Journal Article in ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Engineered endolysin-based 'artilysins' to combat multidrug-resistant Gram-negative pathogens

Yves BriersMaarten WalmaghVictor Van PuyenbroeckAnneleen CornelissenWilliam CenensAbram AertsenHugo OliveiraJoana AzeredoGunther VerweenJean-Paul Pirnay et al.

A1 Journal Article in MBIO

Hurdles in bacteriophage therapy: deconstructing the parameters

Jessica TsonosDieter VandenheuvelYves BriersHenri De GreveJean-Pierre HernalsteensRob Lavigne

A1 Journal Article in VETERINARY MICROBIOLOGY

2013

Characterization of five novel endolysins from Gram-negative infecting bacteriophages

Maarten WalmaghBarbara BoczkowskaBarbara GrymonprezYves BriersZuzanna Drulis-KawaRob Lavigne

A1 Journal Article in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Discovery of an acyclic nucleoside phosphonate that inhibits Mycobacterium tuberculosis ThyX based on the binding mode of a 5-alkynyl substrate analogue

Anastasia ParchinaMatheus FroeyenLia MargamuljanaJef RozenskiSteven De JongheYves BriersRob LavignePiet HerdewijnEveline Lescrinier

A1 Journal Article in CHEMMEDCHEM

2012

Characterization of modular bacteriophage endolysins from Myoviridae phages OBP, 201φ2-1 and PVP-SE1

Maarten WalmaghYves BriersSilvio Branco dos SantosJoana AzeredoRob Lavigne

A1 Journal Article in PLOS ONE

How did bacterial ancestors reproduce?: lessons from L-form cells and giant lipid vesicles

Yves BriersPeter WaldeMarkus SchupplerMartin J Loessner

A1 Journal Article in BIOESSAYS

Intracellular vesicles as reproduction elements in cell wall-deficient L-form bacteria

Yves BriersTitu StaubliMarkus C SchmidMichael WagnerMarkus SchupplerMartin J Loessner

A1 Journal Article in PLOS ONE

2011

Genome sequence of Listeria monocytogenes Scott A, a clinical isolate from a food-borne Listeriosis outbreak

Yves BriersJochen KlumppMarkus SchupplerMartin J Loessner

A1 Journal Article in JOURNAL OF BACTERIOLOGY

The lysis cassette of bacteriophage φKMV encodes a signal-arrest-release endolysin and a pinholin

Yves BriersLiesbet M PeetersGuido VolckaertRob Lavigne

A2 Journal Article in BACTERIOPHAGE

Use of bacteriophage endolysin EL188 and outer membrane permeabilizers against Pseudomonas aeruginosa

Yves BriersM WalmaghR Lavigne

A1 Journal Article in JOURNAL OF APPLIED MICROBIOLOGY

2010

Membrane permeabilization and cellular death of Escherichia coli, Listeria monocytogenes and Saccharomyces cerevisiae as induced by high pressure carbon dioxide treatment

Linsey Garcia-GonzalezAH GeeraerdJ MastYves BriersK ElstL Van GinnekenJF Van ImpeFrank Devlieghere

A1 Journal Article in FOOD MICROBIOLOGY

2009

Listeria monocytogenes L-forms respond to cell wall deficiency by modifying gene expression and the mode of division

Simone Dell'EraCarmen BuchrieserElisabeth CouveBarbara SchnellYves BriersMarkus SchupplerMartin J Loessner

A1 Journal Article in MOLECULAR MICROBIOLOGY

The high-affinity peptidoglycan binding domain of Pseudomonas phage endolysin KZ144

Yves BriersMathias SchmelcherMartin J LoessnerJelle HendrixYves EngelborghsGuido VolckaertRob Lavigne

A1 Journal Article in BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

2008

Analysis of outer membrane permeability of Pseudomonas aeruginosa and bactericidal activity of endolysins KZ144 and EL188 under high hydrostatic pressure

Yves BriersAnneleen CornelissenAbram AertsenKirsten HertveldtChris W MichielsGuido VolckaertRob Lavigne

A1 Journal Article in FEMS MICROBIOLOGY LETTERS

Structural analysis of bacteriophage-encoded peptidoglycan hydrolase domain KMV36C : crystallization and preliminary X-ray diffraction

Kristof Van HeckeYves BriersRita DeruaEtienne WaelkensRob LavigneLuc Van Meervelt

A1 Journal Article in ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS

The genome and structural Proteome of YuA, a new Pseudomonas aeruginosa phage resembling M6

Pieter-Jan CeyssensVadim MesyanzhinovNina SykilindaYves BriersBart RoucourtRob LavigneJohan RobbenArtem DomashinKonstantin MiroshnikovGuido Volckaert et al.

A1 Journal Article in JOURNAL OF BACTERIOLOGY

The structural peptidoglycan hydrolase gp181 of bacteriophage φKZ

Yves BriersKonstantin MiroshnikovOleg ChertkovAlexei NekrasovVadim MesyanzhinovGuido VolckaertRob Lavigne

A1 Journal Article in BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

2007

A standardized approach for accurate quantification of murein hydrolase activity in high-throughput assays

Yves BriersRob LavigneGuido VolckaertKirsten Hertveldt

A1 Journal Article in JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS

Bacteriophage encoded murein hydrolases

Yves BriersGuido VolckaertRob Lavigne

Bookchapter in Contemporary trends in bacteriophage research

Muralytic activity and modular structure of the endolysins of Pseudomonas aeruginosa bacteriophages φKZ and EL

Yves BriersGuido VolckaertAnneleen CornelissenStijn LagaertChris W MichielsKirsten HertveldtRob Lavigne

A1 Journal Article in MOLECULAR MICROBIOLOGY

Stability of phiKMV lysin gp36C reflects its role during bacteriophage infection

Yves BriersRob LavigneKirsten HertveldtIgnace HanssensYves EngelborghsGuido Volckaert

2006

Stability analysis of the bacteriophage φKMV lysin gp36C and its putative role during infection

Yves BriersR LavigneP PlessersK HertveldtI HanssensY EngelborghsG Volckaert

A1 Journal Article in CELLULAR AND MOLECULAR LIFE SCIENCES

The structural proteome of Pseudomonas aeruginosa bacteriophage φKMV

Rob LavigneJean-Paul NobenKirsten HertveldtPieter-Jan CeyssensYves BriersDebora DumontBart RoucourtVictor N KrylovVadim V MesyanzhinovJohan Robben et al.

A1 Journal Article in MICROBIOLOGY-SGM

2004

Identification and characterization of a highly thermostable bacteriophage lysozyme

R LavigneYves BriersK HertveldtJ RobbenG Volckaert

A1 Journal Article in CELLULAR AND MOLECULAR LIFE SCIENCES