Research Explorer

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Researcher

Ward De Spiegelaere

Awards & Distinctions

163 Results

2024

Benchmarking digital PCR partition classification methods with empirical and simulated duplex data

Yao ChenWard De SpiegelaereWim TrypsteenDavid GleerupJo VandesompeleAntoon LievensMatthijs VynckOlivier Thas

U Journal Article in BRIEFINGS IN BIOINFORMATICS

From corrosion casting to virtual dissection : contrast‐enhanced vascular imaging using hafnium oxide nanocrystals

Eline GoossensLoren DeblockLisa CaboorDietger Van den EyndenIván JosipovicPablo Reyes IsaacuraElizaveta MaksimovaMatthias Van ImpeAnne BonninPatrick Segers et al.

A1 Journal Article in SMALL METHODS

2023

Baselining physiological parameters in posture versus locomotion muscles across breeds towards tailored dietary and training management

Carmen Vidal Moreno de VegaConstance De meeûsBerit BoshuizenLorie De MaréYannick GansemansFilip Van NieuwerburghDieter DeforceKlara GoethalsWard De SpiegelaereElisabeth Verdegaal et al.

Digital PCR : a tool in clostridial mutant selection and detection

Evelien DierickChana CallensWard De SpiegelaereRichard DucatelleFilip Van ImmerseelEvy Goossens

A1 Journal Article in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Digital PCR partition classification

Matthijs VynckYao ChenDavid GleerupJo VandesompeleWim TrypsteenAntoon LievensOlivier ThasWard De Spiegelaere

A1 Journal Article in CLINICAL CHEMISTRY

Investigating the impact of orientation of a subcutaneous sensor to its foreign body reaction and fibrotic capsule thickness in pigs using histological analysis

Jeroen CarlierLobke De BelsAsins AznarHanne HuysmansLieven VlaminckWard De SpiegelaereCharlotte Cuypers

Selection and validation of reference genes for RT-qPCR normalization of porcine alveolar macrophages (PAMs) for PRRSV studies

Dayoung OhWard De SpiegelaereHans Nauwynck

A1 Journal Article in SCIENTIFIC REPORTS

The pig as surrogate model for humans for the rational design of an invisible sensor for subcutaneous continuous glucose detection for type 1 diabetes management

Jeroen CarlierMathias DevreeseE Asins AznarHanne HuysmansLieven VlaminckDanaë DelbekeKatrien VanderperrenWard De Spiegelaere

2022

Comparison of serum tryptase as a diagnostic oncological marker in canine versus human mast cell neoplasms

Shana De VosKristel DemeyereHilde De CockNausikaa DevriendtIlona SchwarzkopfRuth FortrieTom RoggemanEvelyne MeyerWard De SpiegelaereHilde De Rooster

A1 Journal Article in RESEARCH IN VETERINARY SCIENCE

Different local, innate and adaptive immune responses are induced by two commercial Mycoplasma hyopneumoniae bacterins and an adjuvant alone

Lisa BeuckelaereMaarten HaspeslaghEvelien BiebautFilip BoyenFreddy HaesebrouckRoman KrejciEvelyne MeyerDavid GleerupWard De SpiegelaereBert Devriendt et al.

A1 Journal Article in FRONTIERS IN IMMUNOLOGY

Differential effect of exercise on bioenergetics and plasticity of the equine muscle

Constance De meeûsCatherine DelesalleLuc LeybaertWard De Spiegelaere

Evaluation of angiogenesis in an acellular porous biomaterial based on polyhydroxybutyrate and chitosan using the chicken ex ovo chorioallantoic membrane model

Zuzana DemcisakovaLenka LuptakovaZuzana TirpakovaAlena KvasilovaLubomir MedveckyWard De SpiegelaereEva Petrovova

A1 Journal Article in CANCERS

First steps towards a representative in vitro tendon model : equine tenocyte seeding on gelatin hydrogels

Marguerite MeeremansLana Van DammeWard De SpiegelaereMario Van PouckeLuc PeelmanSandra Van VlierbergheCatharina De Schauwer

First steps towards a representative in vitro tendon model : equine tenocyte seeding on gelatin hydrogels

Marguerite MeeremansLana Van DammeWard De SpiegelaereMario Van PouckeLuc PeelmanSandra Van VlierbergheCatharina De Schauwer

Intestinal exposure to ceftiofur and cefquinome after intramuscular treatment and the impact of ceftiofur on the pig fecal microbiome and resistome

Sofie RutjensNick VereeckeWard De SpiegelaereSiska CroubelsMathias Devreese

Intestinal exposure to ceftiofur and cefquinome after intramuscular treatment and the impact of ceftiofur on the pig fecal microbiome and resistome

Sofie RutjensNick VereeckeWard De SpiegelaereSiska CroubelsMathias Devreese

A1 Journal Article in ANTIBIOTICS-BASEL

One cisplatin dose provides durable stimulation of anti-tumor immunity and alleviates anti-PD-1 resistance in an intraductal model for triple-negative breast cancer

Jonas SteenbruggeJulie BellemansNiels Vander ElstKristel DemeyereJosephine De VliegherTimothy PereraOlivier De WeverWim Van Den BroeckWard De SpiegelaereNiek Sanders et al.

A1 Journal Article in ONCOIMMUNOLOGY

Qualitative plasma viral load determination as a tool for screening of viral reservoir size in PWH

Thessa LaeremansSigrid D'haeseJonathan AernoutKurt BarbePieter PannusSofie RutsaertEllen VancutsemGuido VanhamCoca NecsoiWard De Spiegelaere et al.

A1 Journal Article in AIDS

The granulation (t)issue : a narrative and scoping review of basic and clinical research of the equine distal limb exuberant wound healing disorder

Nadia Ayurini AnantamaCharis Du CheyneAnn MartensSusanne Pauline RothJanina BurkWard De SpiegelaereJule Kristin Michler

A1 Journal Article in VETERINARY JOURNAL

Triplex digital PCR assays for the quantification of intact proviral HIV-1 DNA

Willem van SnippenbergDavid GleerupSofie RutsaertLinos VandekerckhoveWard De SpiegelaereWim Trypsteen

A1 Journal Article in METHODS

2021

A tale of blood vessels, mononuclear phagocytes and chicken embryos : the chick chorioallantoic membrane assay as model to study macrophages and angiogenesis

Hanna TayWard De SpiegelaereWim Van Den BroeckPieter Cornillie

Comparison of shifts in skeletal muscle plasticity parameters in horses in three different muscles, in answer to 8 weeks of harness training

Constance De meeûsBerit BoshuizenCarmen Vidal Moreno de VegaLuc LeybaertLorie De MaréKlara GoethalsWard De SpiegelaereMaarten OosterlinckCatherine Delesalle

A1 Journal Article in FRONTIERS IN VETERINARY SCIENCE

Depletion of embryonic macrophages leads to a reduction in Angiogenesis in the Ex OVO chick Chorioallantoic membrane assay

Hanna TayCharis Du CheyneKristel DemeyereJurgen De CraeneLobke De BelsEvelyne MeyerAndries ZijlstraWard De Spiegelaere

A1 Journal Article in CELLS

Development of a 3':5' digital PCR assay to determine horse mRNA integrity

Charis Du CheyneYao ChenJurgen De CraeneOlivier ThasWard De Spiegelaere

A1 Journal Article in ANALYTICAL BIOCHEMISTRY

Digital polymerase chain reaction for assessment of mutant mitochondrial carry-over after nuclear transfer for In Vitro fertilization

Olivier TytgatMX TangWillem van SnippenbergAnnekatrien BoelRR GuggillaYannick GansemansMichiel Van HerpSofie SymoensWim TrypsteenDieter Deforce et al.

A1 Journal Article in CLINICAL CHEMISTRY

Digital polymerase chain reaction for assessment of mutant mitochondrial carry-over after nuclear transfer for in vitro fertilization

Olivier TytgatMaoxing TangWillem van SnippenbergAnnekatrien BoelRamesh GuggillaYannick GansemansMichiel Van HerpSofie SymoensWim TrypsteenDieter Deforce et al.

A1 Journal Article in CLINICAL CHEMISTRY

Equine tenocyte seeding on gelatin hydrogels improves elongated morphology

Marguerite MeeremansLana Van DammeWard De SpiegelaereSandra Van VlierbergheCatharina De Schauwer

A1 Journal Article in POLYMERS

Evaluation of the agreement between Brix refractometry and serum immunoglobulin concentration in neonatal piglets

Alexandra SchoosWard De SpiegelaereAn CoolsBart PardonElena Van AudenhoveElise BernaerdtGeert JanssensDominiek Maes

A1 Journal Article in ANIMAL

Flexibility of equine bioenergetics and muscle plasticity in response to different types of training : an integrative approach, questioning existing paradigms

Constance De meeûsBerit BoshuizenMaarten OosterlinckDon van de WinkelWard De SpiegelaereCornelis Marinus de BruijnKlara GoethalsKatrien VanderperrenCatherine Delesalle

A1 Journal Article in PLOS ONE

High numbers of CD163-positive macrophages in the fibrotic region of exuberant granulation tissue in horses

Charis Du CheyneAnn MartensWard De Spiegelaere

A1 Journal Article in ANIMALS

Is there a rationale to supplement hepatotropic factors to dogs with multiple acquired portosystemic shunts secondary to congenital portosystemic shunt attenuation? = Is er wetenschappelijke basis om hepatotrope factoren te supplementeren aan honden met multipele verworven portosystemische shunts secundair aan attenuatie van een congenitale portosystemische shunt?

Lisa Van AckerHilde De RoosterGonçalo Dos Santos Simões SerranoDominique PaepeWard De SpiegelaereNausikaa Devriendt

A1 Journal Article in VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT

Measuring proviral HIV-1 DNA : hurdles and improvements to an assay monitoring integration events utilising human alu repeat sequences

Eva MalatinkováJordan ThomasWard De SpiegelaereSofie RutsaertAnna Maria GerettiGeorgios PollakisWilliam A. PaxtonLinos VandekerckhoveAlessandra Ruggiero

A1 Journal Article in LIFE-BASEL

Paternally expressed retrotransposon Gag-like 1 gene, RTL1, is one of the crucial elements for placental angiogenesis in horses

Pouya DiniM CarossinoBalasuriya UBRH El-Sheikh AliSC LouxA Esteller-VicoKE ScogginAT LoynachanT KalbfleischWard De Spiegelaere et al.

A1 Journal Article in BIOLOGY OF REPRODUCTION

Serial block-face scanning electron microscopy (SBF-SEM) of equine exuberant granulation tissue

Charis Du CheyneLiesbeth CouckAnneke KremerEva PintAnn MartensWard De Spiegelaere

Techniques used to assess intussusceptive angiogenesis : a systematic review

Charis Du CheyneMarloes SmeetsWard De Spiegelaere

A1 Journal Article in DEVELOPMENTAL DYNAMICS

The exuberant granulation (t)issue : what triggers distal limb wounds in horses to go out of control

Charis Du CheyneWard De SpiegelaereAnn Martens

2020

Chorioallantoic membrane assay as model for angiogenesis in tissue engineering : focus on stem cells

Greet MerckxHanna TayMelissa Lo MonacoMarc van ZandvoortWard De SpiegelaereIvo LambrichtsAnnelies Bronckaers

A1 Journal Article in TISSUE ENGINEERING PART B-REVIEWS

Corrosion casting of the cardiovascular structure in adult zebrafish for analysis by scanning electron microscopy and X‐ray microtomography

Ward De SpiegelaereLisa CaboorMatthias Van ImpeMatthieu BooneJulie De BackerPatrick SegersPatrick Sips

A1 Journal Article in ANATOMIA HISTOLOGIA EMBRYOLOGIA

Rapid viral rebound after analytical treatment interruption in patients with very small HIV reservoir and minimal on-going viral transcription

Pieter PannusSofie RutsaertStéphane De WitSabine D. AllardGuido VanhamBasiel ColeCoca NescoiJoeri AertsWard De SpiegelaereAchilleas Tsoumanis et al.

A1 Journal Article in JOURNAL OF THE INTERNATIONAL AIDS SOCIETY

The complex TIE between macrophages and angiogenesis

Charis Du CheyneHanna TayWard De Spiegelaere

A1 Journal Article in ANATOMIA HISTOLOGIA EMBRYOLOGIA

The digital MIQE guidelines update : minimum information for publication of quantitative digital PCR experiments for 2020

Jim F. HuggettAlexandra S. WhaleWard De SpiegelaereAfif Abdel NourYoung-Kyung BaeVladimir BenesDaniel BurkeMegan ClevelandPhilippe CorbisierAlison S. Devonshire et al.

A1 Journal Article in CLINICAL CHEMISTRY

Total HIV-1 DNA as a marker of the viral reservoir

Sofie RutsaertLinos VandekerckhoveWard De Spiegelaere

Underestimated effect of intragenic HIV-1 DNA methylation on viral transcription in infected individuals

Sam KintWim TrypsteenWard De SpiegelaereEva MalatinkováSabine Kinloch-de LoesTim De MeyerWim Van CriekingeLinos Vandekerckhove

A1 Journal Article in CLINICAL EPIGENETICS

2019

Analytical treatment interruption (ATI) in patients with very small HIV reservoir

Pieter PannusSofie RutsaertStéphane De WitGuido VanhamCoca NecsoiJoeri AertsWard De SpiegelaereAchilleas TsoumanisMarie CouttenyeNatacha Herssens et al.

Blood vessels in exuberant granulation tissue in horses : a histological analysis

Charis Du CheyneTanja HaccuriaLiesbeth CouckEva PintAnn MartensWard De Spiegelaere

Early treated HIV-1 positive individuals demonstrate similar restriction factor expression profile as long-term non-progressors

Clarissa Van HeckeWim TrypsteenEva MalatinkováWard De SpiegelaereKaren VervischSofie RutsaertSabine Kinloch-de LoesMagdalena SipsLinos Vandekerckhove

A1 Journal Article in EBIOMEDICINE

Evaluation of HIV-1 reservoir levels as possible markers for virological failure during boosted darunavir monotherapy

Sofie RutsaertWard De SpiegelaereLaura De ClercqLinos Vandekerckhove

A1 Journal Article in JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

In-vitro viral suppressive capacity correlates with immune checkpoint marker expression on peripheral CD8+ T cells in treated HIV-positive patients

Pieter PannusPhilipp AdamsElisabeth WillemsLeo HeyndrickxEric FlorenceSofie RutsaertWard De SpiegelaereLinos VandekerckhoveCarole Seguin-DevauxGuido Vanham

A1 Journal Article in AIDS

Keelsnijding bij noodslachting van runderen

Lieven De ZutterWard De Spiegelaere

Editorial material

Long non-coding RNAs and latent HIV : a search for novel targets for latency reversal

Wim TrypsteenCory H WhiteAmey MukimCelsa A SpinaWard De SpiegelaereSteve LefeverChristopher H WoelkAlberto BosqueLinos VandekerckhoveNadejda Beliakova-Bethell

Long non-coding RNAs and latent HIV : a search for novel targets for latency reversal

Wim TrypsteenCory H WhiteAmey MukimCelsa A SpinaWard De SpiegelaereSteve LefeverVicente PlanellesAlberto BosqueChristopher H WoelkLinos Vandekerckhove et al.

A1 Journal Article in PLOS ONE

Power Doppler ultrasound and contrast-enhanced ultrasound demonstrate non-invasive tumour vascular response to anti-vascular therapy in canine cancer patients

Eline AbmaEmmelie StockWard De SpiegelaereLeen Van BrantegemKatrien VanderperrenYicheng NiMatthijs VynckSylvie DaminetKaat De ClercqHilde De Rooster

A1 Journal Article in SCIENTIFIC REPORTS

2018

A single dose of intravenous combretastatin A4-phosphate is reasonably well tolerated and significantly reduces tumour vascularization in canine spontaneous cancers

Eline AbmaWard De SpiegelaereKatrien VanderperrenEmmelie StockLeen Van BrantegemIne CornelisSylvie DaminetY NiMatthijs VynckGlenn Verstraete et al.

A1 Journal Article in VETERINARY AND COMPARATIVE ONCOLOGY

Beneficial impact of early treatment on restriction factor expression profile

Clarissa Van HeckeMagdalena SipsEva MalatinkováWard De SpiegelaereKaren VervischMargaret JohnsonSabine Kinloch-de LoesWim TrypsteenLinos Vandekerckhove

Beneficial impact of early treatment on restriction factor expression profile

Clarissa Van HeckeWim TrypsteenEva MalatinkováWard De SpiegelaereKaren VervischMargaret JohnsonSabine Kinloch-de LoesMagdalena SipsLinos Vandekerckhove

Depletion of macrophages in the ex ovo chick embryo chorioallantoic membrane model

Hanna TayWim Van Den BroeckJurgen De CraeneWard De Spiegelaere

Evaluation of bisulfite kits for DNA methylation profiling in terms of DNA fragmentation and DNA recovery using digital PCR

Sam KintWard De SpiegelaereJonas De KeselLinos VandekerckhoveWim Van Criekinge

A1 Journal Article in PLOS ONE

Expression of angiopoietin receptors TIE2 and TIE1 in equine exuberant granulation tissue

Charis Du CheyneAnn MartensWard De Spiegelaere

In-depth validation of total HIV-1 DNA assays for quantification of various HIV-1 subtypes

Sofie RutsaertWard De SpiegelaereClarissa Van HeckeMarie-Angélique De ScheerderMaja KiselinovaKaren VervischWim TrypsteenLinos Vandekerckhove

A1 Journal Article in SCIENTIFIC REPORTS

Intussusceptive pillar formation in developing porcine glomeruli

Anastasia LogothetidouWard De SpiegelaereTim VandecasteeleWaltraud TschulenkIngrid WalterWim Van Den BroeckPieter Cornillie

A1 Journal Article in JOURNAL OF VASCULAR RESEARCH

Standardizing digital PCR diagnostics and pioneering long non-coding RNA discovery in the context of HIV cure research

Wim TrypsteenLinos VandekerckhoveWard De Spiegelaere

Treatment of a malignant peripheral nerve sheath tumor by intravenous administration of combretastatin A4-phosphate in a dog

Shana De VosEline AbmaKatrien VanderperrenLeen Van BrantegemWard De SpiegelaereHilde De Rooster

A1 Journal Article in VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT

2017

Beneficial impact of early treatment on restriction factor expression profile

Clarissa Van HeckeMagdalena SipsEva MalatinkováWard De SpiegelaereKaren VervischMargaret JohnsonSabine Kinloch-de LoesWim TrypsteenLinos Vandekerckhove

Challenges of in vitro HIV latency models

Pawel BonczkowskiLinos VandekerckhoveWard De Spiegelaere

Cross-validation of HIV DNA assays to quantify different HIV-1 subtypes

Sofie RutsaertWard De SpiegelaereClarissa Van HeckeMarie-Angélique De ScheerderMaja KiselinovaKaren VervischWim TrypsteenLinos Vandekerckhove

Defining a Total HIV DNA threshold as guidance for therapy simplification strategies

Sofie RutsaertIngeborg WijtingWard De SpiegelaereLaura De ClercqBart RijndersLinos Vandekerckhove

Differential expression of lncRNAs during the HIV replication cycle: an underestimated layer in the HIV-host interplay (vol 6, 36111, 2016)

Wim TrypsteenPejman MohammadiClarissa Van HeckePieter MestdaghSteve LefeverYvan SaeysPieter De BleserJo VandesompeleAngela CiuffiLinos Vandekerckhove et al.

HIV DNA as a predictive marker for virological failure of darunavir/r monotherapy : a substudy of the PROTEA trial to define a cut-off for succes

Sofie RutsaertWard De SpiegelaereLaura De ClercqLinos Vandekerckhove

HIV integration sites in latently infected cell lines : evidence of ongoing replication

Jori SymonsAbha ChopraEva MalatinkováWard De SpiegelaereShay LearyDon CooperChike O AbanaAjantha RhodesSimin D RezaeiLinos Vandekerckhove et al.

A1 Journal Article in RETROVIROLOGY

HIV reservoir characterization symposium 19 September 2016, Ghent, Belgium

Eva MalatinkováWard De SpiegelaereLinos VandekerckhoveMagdalena Sips

A1 Journal Article in JOURNAL OF VIRUS ERADICATION

Immunohistochemical identification of stent-based ablation lesions in the superior vena cava and pulmonary veins

Tim VandecasteeleStijn SchauvliegeTim BoussyMatthew PhilpottEli ClementLisse VeraPieter CornillieWard De SpiegelaereGlenn Van LangenhoveGunther van Loon et al.

A2 Journal Article in JOURNAL OF HISTOLOGY & HISTOPATHOLOGY

Intussusceptive angiogenesis in the developing porcine kidney : morphologic characterization of the initial phases and the involvement of angiopoietin receptors

Anastasia LogothetidouPieter CornillieWard De Spiegelaere

Short-course Toll-like receptor 9 agonist treatment impacts innate immunity and plasma viremia in individuals with human immunodeficiency virus infection

Line VibholmMariane H SchleimannJesper F HøjenThomas BenfieldRasmus OffersenKatrine RasmussenRikke OlesenAnders DigeJørgen AgnholtJudith Grau et al.

A1 Journal Article in CLINICAL INFECTIOUS DISEASES

Stereological and immunogold studies on TIE1 and TIE2 localization in glomeruli indicate angiopoietin signaling in podocytes

Anastasia LogothetidouWard De SpiegelaereWim Van Den BroeckTim VandecasteeleLiesbeth CouckPaul SimoensPieter Cornillie

A1 Journal Article in MICRON

Utility of integrated HIV-1 DNA quantification in cure studies

Alessandra RuggieroEva MalatinkováSofie RutsaertWilliam A PaxtonLinos VandekerckhoveWard De Spiegelaere

A1 Journal Article in FUTURE VIROLOGY

lncRNA discovery in the HIV replication cycle adds new layer in HIV-host interplay

Wim TrypsteenMohammadi PejmanClarissa Van HeckePieter MestdaghSteve LefeverPieter De BleserJo VandesompeleAngela CiuffiWard De SpiegelaereLinos Vandekerckhove

2016

Diagnostic utility of droplet digital PCR for HIV reservoir quantification

Wim TrypsteenMaja KiselinovaLinos VandekerckhoveWard De Spiegelaere

A2 Journal Article in JOURNAL OF VIRUS ERADICATION

Differential expression of lncRNAs during the HIV replication cycle : an underestimated layer in the HIV-host interplay

Wim TrypsteenPejman MohammadiClarissa Van HeckePieter MestdaghSteve LefeverYvan SaeysPieter De BleserJo VandesompeleAngela CiuffiLinos Vandekerckhove et al.

A1 Journal Article in SCIENTIFIC REPORTS

In search of predictive biomarkers of the replication-competent HIV-1 reservoir

Maja KiselinovaLinos VandekerckhoveWard De Spiegelaere

Integrated and total HIV-1 DNA predict ex vivo viral outgrowth

Maja KiselinovaWard De SpiegelaereMaria Jose BuzonEva MalatinkováMathias LichterfeldLinos Vandekerckhove

A1 Journal Article in PLOS PATHOGENS

Laser capture microdissection of intestinal tissue from sea bass larvae using an optimized RNA integrity assay and validated reference genes

Marlien SchaeckWard De SpiegelaereJurgen De CraeneWim Van Den BroeckBart De SpiegeleerChristian BurvenichFreddy HaesebrouckAnnemie Decostere

A1 Journal Article in SCIENTIFIC REPORTS

Minimal requirements for primary HIV latency models based on a systematic review

Pawel BonczkowskiMarie-Angélique De ScheerderWard De SpiegelaereLinos Vandekerckhove

A1 Journal Article in AIDS REVIEWS

Modeling HIV-1 latency in primary T cells using a replication-competent virus

Laura MartinsPawel BonczkowskiAdam M SpivakWard De SpiegelaereCamille L NovisAna Beatriz DePaula-SilvaEva MalatinkováWim TrypsteenAlberto BosqueLinos Vandekerckhove et al.

A1 Journal Article in AIDS RESEARCH AND HUMAN RETROVIRUSES

Prospects for digital PCR in viral diagnostics

Ward De Spiegelaere

Protein expression from unintegrated HIV-1 DNA introduces bias in primary in vitro post-integration latency models

Pawel BonczkowskiMarie-Angélique De ScheerderEva MalatinkováAlexandra BorchZora MelkovaRenate KoenigWard De SpiegelaereLinos Vandekerckhove

A1 Journal Article in SCIENTIFIC REPORTS

Quantitation of tumor angiogenesis in vitro : an all-in-one angiogenesis assay

Mahtab BahramsoltaniWard De Spiegelaere

Bookchapter in Tumor angiogenesis assays : methods and protocols

The future of digital polymerase chain reaction in virology

Matthijs VynckWim TrypsteenOlivier ThasLinos VandekerckhoveWard De Spiegelaere

A1 Journal Article in MOLECULAR DIAGNOSIS & THERAPY

The long lasting impact of early treatment initiation on the size and dynamics of HIV-1 reservoirs

Eva MalatinkováLinos VandekerckhoveWard De Spiegelaere

The use of HIV-1 integration site analysis information in clinical studies aiming at HIV cure

Maja KiselinovaWard De SpiegelaereLinos Vandekerckhove

A2 Journal Article in JOURNAL OF VIRUS ERADICATION

2015

Accurate quantification of episomal HIV-1 two-long terminal repeat circles by use of optimized DNA isolation and droplet digital PCR

Eva MalatinkováMaja KiselinovaPawel BonczkowskiWim TrypsteenPeter MessiaenJolien VermeireBruno VerhasseltKAREN VERVISCHLinos VandekerckhoveWard De Spiegelaere

A1 Journal Article in JOURNAL OF CLINICAL MICROBIOLOGY

Aviremia 10 years postdiscontinuation of antiretroviral therapy initiated during primary human immunodeficiency virus-1 infection and association with gag-specific T-cell responses

Sabine Kinloch-de LoesLucy DorellHongbing YangGareth AD HardySabine YerlyCristina CelleraiLinos VandekerckhoveWard De SpiegelaereEva MalatinkováWillie Wee Lee Koh et al.

A1 Journal Article in OPEN FORUM INFECTIOUS DISEASES

Aviremia 10-year post-ART discontinuation initiated at seroconversion

Sabine KinlochLucy DorrelHongbing YangLinos VandekerckhoveWard De SpiegelaereEva MalatinkovaSabine YerlyDaniel WebsterMargaret Johnson

Comparison of digital PCR platforms and semi-nested qPCR as a tool to determine the size of the HIV reservoir

Kobus J BosmanMonique NijhuisPetra M Van HamAnnemarie MJ WensingKAREN VERVISCHLinos VandekerckhoveWard De Spiegelaere

A1 Journal Article in SCIENTIFIC REPORTS

Comparison of methods for in-house screening of HLA*B57:01 to prevent abacavir hypersensitivity in HIV-1 care

Ward De SpiegelaereJan PhilippéKAREN VERVISCHChris VerhofstedeEva MalatinkováMaja KiselinovaWim TrypsteenPawel BonczkowskiDirk VogelaersSteven Callens et al.

A1 Journal Article in PLOS ONE

Comprehensive longitudinal characterization of HIV-1 reservoir markers in patients on stable antiretroviral treatment

Maja KiselinovaWard De SpiegelaereMaria Jose BuzonEva MalatinkováMathias LichterfeldLinos Vandekerckhove

Considerations for low level DNA detection with dPCR: quantifying the HIV reservoir markers

Ward De Spiegelaere

During stably suppressive antiretroviral therapy integrated HIV-1 DNA load in peripheral blood is associated with the frequency of CD8 cells expressing HLA-DR/DP/DQ

Alessandra RuggieroWard De SpiegelaereAlessandro Cozzi-LepriMaja KiselinovaGeorgios PollakisApostolos BeloukasLinos VandekerckhoveMatthew StrainDouglas RichmanAndrew Phillips et al.

A1 Journal Article in EBIOMEDICINE

Extreme value theory for threshold determination in droplet digital PCR experiments

Matthijs VynckWim TrypsteenJan De NevePawel BonczkowskiMaja KiselinovaEva MalatinkováKAREN VERVISCHOlivier ThasLinos VandekerckhoveWard De Spiegelaere

Fluoxetine suppresses calcium signaling in human T lymphocytes through depletion of intracellular calcium stores

Veerle GobinMarijke De BockBart BroeckxMaja KiselinovaWard De SpiegelaereLinos VandekerckhoveKatleen Van SteendamLuc LeybaertDieter Deforce

A1 Journal Article in CELL CALCIUM

HIV-1 RNA and HIV-1 DNA persistence during suppressive ART with PI-based or nevirapine-based regimens

Maja KiselinovaAnna Maria GerettiEva MalatinkováKAREN VERVISCHApostolos BeloukasPeter MessiaenPawel BonczkowskiWim TrypsteenSteven CallensChris Verhofstede et al.

A1 Journal Article in JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

Impact of a decade of successful antiretroviral therapy initiated at HIV-1 seroconversion on blood and mucosal reservoirs

Eva MalatinkováWard De SpiegelaerePawel BonczkowskiMaja KiselinovaWim TrypsteenMargaret JohnsonChris VerhofstedeDanny De LoozeCharles MurraySabine Kinloch-de Loes et al.

A1 Journal Article in ELIFE

Impact of a decade of successful antiretroviral therapy started during HIV-1 seroconversion on blood and gut reservoirs

Sabine Kinloch-de LoesEva MalatinkováWard De SpiegelaerePawel BonczkowskiMaja KiselinovaKAREN VERVISCHWim TrypsteenMargaret JohnsonDanny De LoozeLinos Vandekerckhove

Integrated and total HIV-1 DNA can predict quantitative viral outgrowth in patients on long-term ART

Ward De SpiegelaereMaja KiselinovaMaria J BuzonEva MalatinkováMathias LichterfeldLinos Vandekerckhove

Integration site analysis of latently infected cell lines: evidence of ongoing replication

Jory SymonsSharon LewinAbha ChopraEva MalatinkováWard De SpiegelaereShay LearyDavid CooperLinos VandekerckhoveSimon MallalPaul Cameron

Long-term early antiretroviral therapy limits the HIV-1 reservoir size as compared to later treatment initiation but not to levels found in long-term non-progressors

Eva MalatinkováWard De SpiegelaerePawel BonczkowskiMaja KiselinovaKAREN VERVISCHWim TrypsteenMargaret JohnsonChris VerhofstedeDanny De LoozeCharles Murray et al.

Reference gene validation for RT-qPCR, a note on different available software packages

Ward De SpiegelaereJutta Dern-WielochRoswitha WeigelValérie SchumacherHubert SchorleDaniel NettersheimMartin BergmannRalph BrehmSabine KlieschLinos Vandekerckhove et al.

A1 Journal Article in PLOS ONE

Robust regression methods for real-time PCR

Wim TrypsteenJan De NeveKobus BosmanMonique NijhuisOlivier ThasLinos VandekerckhoveWard De Spiegelaere

Robust regression methods for real-time polymerase chain reaction

Wim TrypsteenJan De NeveKobus BosmanMonique NijhuisOlivier ThasLinos VandekerckhoveWard De Spiegelaere

A1 Journal Article in ANALYTICAL BIOCHEMISTRY

Threshold determination in droplet digital PCR experiments using extreme value theory

Matthijs VynckWim TrypsteenJan De NevePawel BonczkowskiMaja KiselinovaEva MalatinkováKAREN VERVISCHOlivier ThasLinos VandekerckhoveWard De Spiegelaere

ddcRquant: threshold determination for single channel droplet digital PCR experiments

Wim TrypsteenMatthijs VynckJan De NevePawel BonczkowskiMaja KiselinovaEva MalatinkováKAREN VERVISCHOlivier ThasLinos VandekerckhoveWard De Spiegelaere

ddpcRquant: threshold determination for single channel droplet digital PCR experiments

Wim TrypsteenMatthijs VynckJan De NevePawel BonczkowskiMaja KiselinovaEva MalatinkováKAREN VERVISCHOlivier ThasLinos VandekerckhoveWard De Spiegelaere

A1 Journal Article in ANALYTICAL AND BIOANALYTICAL CHEMISTRY

2014

Accurate episomal HIV 2-LTR circles quantification using optimized DNA isolation and droplet digital PCR

Eva MalatinkováMaja KiselinovaPawel BonczkowskiWim TrypsteenPeter MessiaenJolien VermeireBruno VerhasseltKaren VervischLinos VandekerckhoveWard De Spiegelaere

Angiogenesis and collagen type IV expression in different endothelial cell culture systems

Mahtab BahramsoltaniIlka SlosarekWard De SpiegelaereJohanna Plendl

A1 Journal Article in ANATOMIA HISTOLOGIA EMBRYOLOGIA

Antiretrovirals for HIV prevention: when should they be recommended?

Maja KiselinovaWard De SpiegelaereChris VerhofstedeSteven CallensLinos Vandekerckhove

A1 Journal Article in EXPERT REVIEW OF ANTI-INFECTIVE THERAPY

Characterization of serotonin transporter expression in human T lymphocytes

Veerle GobinKatleen Van SteendamMaja KiselinovaWard De SpiegelaereLinos VandekerckhoveDieter Deforce

Comparison of droplet digital PCR and seminested real-time PCR for quantification of cell-associated HIV-1 RNA

Maja KiselinovaAlexander O PasternakWard De SpiegelaereDirk VogelaersBen BerkhoutLinos Vandekerckhove

A1 Journal Article in PLOS ONE

Evaluation of normalization strategies used in real-time quantitative PCR experiments in HepaRG cell line studies

Liesbeth CeelenJurgen De CraeneWard De Spiegelaere

A1 Journal Article in CLINICAL CHEMISTRY

Quantification of integrated HIV DNA by repetitive-sampling Alu-HIV PCR on the basis of Poisson statistics

Ward De SpiegelaereEva MalatinkováLindsay LynchFilip Van NieuwerburghPeter MessiaenUna O'DohertyLinos Vandekerckhove

A1 Journal Article in CLINICAL CHEMISTRY

Replication competent virus as an important source of bias in HIV latency models utilizing single round viral constructs

Pawel BonczkowskiWard De SpiegelaereAlberto BosqueCory H WhiteAnouk Van NuffelEva MalatinkováMaja KiselinovaWim TrypsteenWojciech WitkowskiJolien Vermeire et al.

A1 Journal Article in RETROVIROLOGY

The impact of nevirapine- versus protease inhibitor-based regimens on virological markers of HIV-1 persistence during seemingly suppressive ART

Maja KiselinovaMaria AnnaEva MalatinkováKAREN VERVISCHApostolos BeloukasPeter MessiaenPawel BonczkowskiWim TrypsteenSteven CallensChris Verhofstede et al.

2013

Droplet digital PCR, the new tool in HIV reservoir quantification?

Ward De SpiegelaereMaja KiselinovaEva MalatinkováAlexander PasternakBen BerkhoutLinos Vandekerckhove

Porcine reproductive and respiratory syndrome virus infection is associated with an increased number of Sn-positive and CD8-positive cells in the maternal-fetal interface

Uladzimir KarniychukWard De SpiegelaereHans Nauwynck

A1 Journal Article in VIRUS RESEARCH

Quantification of integrated HIV DNA by repetitive sampling Alu-HIV PCR and Poisson statistics

Ward De SpiegelaereEva MalatinkováFilip Van NieuwerburghUna O'DohertyLinos Vandekerckhove

The need for transparency and good practices in the qPCR literature

Stephen A BustinVladimir BenesJeremy GarsonJan HellemansJim HuggettMikael KubistaReinhold MuellerTania NolanMichael W PfafflGregory Shipley et al.

A1 Journal Article in NATURE METHODS

Touchdown digital PCR for quantification of difficult amplicons in the HIV genome

Ward De SpiegelaereEva MalatinkováMaja KiselinovaPawel BonczkowskiChris VerhofstedeDirk VogelaersLinos Vandekerckhove

Touchdown digital polymerase chain reaction for quantification of highly conserved sequences in the HIV-1 genome

Ward De SpiegelaereEva MalatinkováMaja KiselinovaPawel BonczkowskiChris VerhofstedeDirk VogelaersLinos Vandekerckhove

A1 Journal Article in ANALYTICAL BIOCHEMISTRY

Transmission electron microscopy of endothelial cell cultures

Els Van MulkenWard De SpiegelaereWim Van Den BroeckPieter Cornillie

2012

Characterization of LEDGF/p75 genetic variants and association with HIV-1 disease progression

Peter MessiaenWard De SpiegelaereJose AlcamiKaren VervischPetra Van AckerBruno VerhasseltPieter MeuwissenEsther CalongeNuria GonzalezFelix Gutierrez-Rodero et al.

A1 Journal Article in PLOS ONE

Comprehensive characterization of LEDGF/p75 in a HIV-1 infected patient cohort

Peter MessiaenWard De SpiegelaereJose AlcamiPaul CouckePetra Van AckerKaren VervischBruno VerhasseltPieter MeeuwissenBruce PoppeDirk Vogelaers et al.

Differential microRNA expression analysis in blastocysts by whole mount in situ hybridization and reverse transcription quantitative polymerase chain reaction on laser capture microdissection samples

Karen GoossensWard De SpiegelaereMieke StevensChristian BurvenichBart De SpiegeleerPieter CornillieAlex Van ZeverenAnn Van SoomLuc Peelman

A1 Journal Article in ANALYTICAL BIOCHEMISTRY

Gene expression analysis in specific cell populations of bovine blastocysts using laser capture microdissection

Ward De SpiegelaereKaren GoossensMieke StevensAnn Van SoomLuc PeelmanChristian BurvenichBart De SpiegeleerPieter Cornillie

Intussusceptive angiogenesis: a biologically relevant form of angiogenesis

Ward De SpiegelaereChristophe CasteleynWim Van Den BroeckJohanna PlendlMahtab BahramsoltaniPaul SimoensValentin DjonovPieter Cornillie

A1 Journal Article in JOURNAL OF VASCULAR RESEARCH

Laser capture microdissection for gene expression analysis of specific cell populations in single blastocysts

Ward De SpiegelaereMuriel FilliersAnn Van Soom

Bookchapter in Single cell analysis : methods and protocols

2011

Angiopoietins differentially influence in vitro angiogenesis by endothelial cells of different origin

Ward De SpiegelaerePieter CornillieWim Van Den BroeckJohanna PlendlMahtab Bahramsoltani

A1 Journal Article in CLINICAL HEMORHEOLOGY AND MICROCIRCULATION

Building the glomerular vasculature: multiple roles for VEGFA and the angiopoietins during angiogenesis

Ward De SpiegelaereWim Van Den BroeckPieter Cornillie

Comprehensive characterization of LEDGF/p75 in a HIV-1-infected patient cohort

Peter MessiaenWard De SpiegelaereChris VerhofstedePaul CouckePetra Van AckerPhilip VlummensKAREN VERVISCHDirk VogelaersBruce PoppeJose Alcami et al.

Critical selection of reliable reference genes for gene expression study in the HepaRG cell line

Liesbeth CeelenWard De SpiegelaereMichael DavidJurgen De CraeneMathieu VinkenTamara VanhaeckeVera Rogiers

A1 Journal Article in BIOCHEMICAL PHARMACOLOGY

Differences between the ovine tonsils based on an immunohistochemical quantification of the lymphocyte subpopulations

Sofie BreugelmansWard De SpiegelaereChristophe CasteleynPaul SimoensWim Van Den Broeck

A1 Journal Article in COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES

Distribution of the Shadoo protein in the ovine brain assessed by immunohistochemistry

Evelyne LampoWim Van Den BroeckNicolas WillemarckMario Van PouckeChristophe CasteleynWard De SpiegelaereAlex Van ZeverenLuc Peelman

A1 Journal Article in RESEARCH IN VETERINARY SCIENCE

Immunohistochemical detection of the angiopoietins during porcine metanephric kidney development

Ward De SpiegelaerePieter CornilliePaul SimoensWim Van Den Broeck

A1 Journal Article in ACTA HISTOCHEMICA

Laser capture microdissection for gene expression analysis of inner cell mass and trophectoderm from blastocysts

Muriel FilliersWard De SpiegelaereLuc PeelmanKaren GoossensChristian BurvenichLeen VandaelePieter CornillieAnn Van Soom

A1 Journal Article in ANALYTICAL BIOCHEMISTRY

Laser capture microdissection for gene expression analysis of inner cell mass and trophoblast from bovine blastocysts

Muriel FilliersWard De SpiegelaereLuc PeelmanKaren GoossensChristian BurvenichLeen VandaelePieter CornillieAnn Van Soom

Quantitative mRNA expression analysis in kidney glomeruli using microdissection techniques

Ward De SpiegelaerePieter CornillieMario Van PouckeLuc PeelmanChristian BurvenichWim Van Den Broeck

A1 Journal Article in HISTOLOGY AND HISTOPATHOLOGY

Simple and quick method for whole-liver decellularization: a novel in vitro three-dimensional bioengineering tool?

Joery De KockLiesbeth CeelenWard De SpiegelaereChristophe CasteleynPaul ClaesTamara VanhaeckeVera Rogiers

A1 Journal Article in ARCHIVES OF TOXICOLOGY

Stereology and computer assisted three-dimensional reconstruction as tools to study probiotic effects of Aeromonas hydrophila on the digestive tract of germ-free Artemia franciscana nauplii

Rangalle GunasekaraPieter CornillieChristophe CasteleynWard De SpiegelaerePatrick SorgeloosPaul SimoensPeter BossierWim Van Den Broeck

A1 Journal Article in JOURNAL OF APPLIED MICROBIOLOGY

Stimulation of angiogenesis in a 3D spheroid sprouting assay with glomerular endothelial cells

Ward De SpiegelaerePieter CornillieJurgen De CraeneWim Van Den Broeck

2010

Comparative study of the influence of mnn9 yeast vs. wild type yeast on the development of the digestive tract of Artemia franciscana nauplii

Rangalle GunasekaraAnamaria RekeckiSri Kartik BaruahNatrah Fatin Mohd IkhsanWard De SpiegelaerePeter BossierWim Van Den Broeck

Detection of hypoxia inducible factors and angiogenic growth factors during foetal endochondral and intramembranous ossification

Ward De SpiegelaerePieter CornillieChristophe CasteleynChristian BurvenichWim Van Den Broeck

A1 Journal Article in ANATOMIA HISTOLOGIA EMBRYOLOGIA

Expression and localization of angiogenic growth factors in developing porcine mesonephric glomeruli

Ward De SpiegelaerePieter CornillieTim ErkensDenis Van LooChristophe CasteleynMario Van PouckeChristian BurvenichLuc Van HoorebekeChris Van GinnekenLuc Peelman et al.

A1 Journal Article in JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY

Expression of angiogenic growth factors during mesonephric glomerulogenesis

Ward De SpiegelaerePieter CornillieMario Van PouckeLuc PeelmanChristian BurvenichPaul SimoensWim Van Den Broeck

Localization of erythropoietin in and around growing cartilage

Ward De SpiegelaerePieter CornillieWim Van Den Broeck

A1 Journal Article in MOLECULAR AND CELLULAR BIOCHEMISTRY

Quantitation of angiogenesis in vitro induced by VEGF-A and FGF-2 in two different human endothelial cultures : an all-in-one assay

Mahtab BahramsoltaniWard De SpiegelaerePawel JanczykBernhard HieblPieter CornillieJohanna Plendl

A1 Journal Article in CLINICAL HEMORHEOLOGY AND MICROCIRCULATION

Quantitative gene expression analysis on small samples obtained with laser capture microdissection

Ward De Spiegelaere

2009

Expression of angiogenic growth factors in porcine mesonephric glomeruli, isolated with Laser Capture Microdissection

Ward De SpiegelaerePieter CornillieTim ErkensMario Van PouckeLuc PeelmanChristian BurvenichWim Van Den Broeck

Morphologische und quantitative in vitro-Studie zum proangiogenen Effekt von VEGF und FGF-2in zwei verschiedenen humanen Endothelzellkulturen

M. BahramsoltaniWard De SpiegelaereP. JanczykB. HieblPieter CornillieJ. Plendl

On the function of epithelial cells with large cave-like depressions present on the lateral choroid plexus

Ward De SpiegelaereChristophe CasteleynWim Van Den BroeckPaul Simoens

Spider fauna in a grass dune remnant at the black sea coast (Romania), presenting seven new species for the romanian fauna including the first description of the female Harpactea alexandrae lazarov, 2006 (Dysderidae)

Ward De SpiegelaereRobert Bosmans

A2 Journal Article in Analele Stiintifice ale Universitatii “Al. I. Cuza” Iasi, s. Biologie animala

The expression of angiopoietins during glomerular development in the porcine mesonephros

Ward De SpiegelaerePieter CornillieTim ErkensChristian BurvenichLuc PeelmanWim Van Den Broeck

2008

Electron microscopic study of the porcine choroid plexus epithelium

Ward De SpiegelaereChristophe CasteleynWim Van Den BroeckPaul Simoens

A1 Journal Article in ANATOMIA HISTOLOGIA EMBRYOLOGIA

Elimination of amplification artifacts in real-time reverse transcription PCR using laser capture microdissected samples

Ward De SpiegelaereTim ErkensJurgen De CraeneChristian BurvenichLuc PeelmanWim Van Den Broeck

A1 Journal Article in ANALYTICAL BIOCHEMISTRY

Gene expression analysis of growth factors in glomerulogenesis using Laser Capture Microdissection

Ward De SpiegelaereTim ErkensJurgen De CraeneChristian BurvenichWim Van Den Broeck

RNA-isolation and gene expression of growth factors in embryonic tissue sections using Laser Capture Microdissection

Ward De SpiegelaereChristian BurvenichWim Van Den Broeck

2006

Zelotes balcanicus sp n., a new and widespread species from the Balkan Peninsula (Araneae, Gnaphosidae) - A new Zelotes species

C DELTSHEVRobert BosmansWard De Spiegelaere

A1 Journal Article in REVUE SUISSE DE ZOOLOGIE