Research Explorer

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Researcher

Tom Jacobs

Awards & Distinctions

42 Results

2024

Continual improvement of CRISPR‐induced multiplex mutagenesis in Arabidopsis

Ward DeveltereWard DecaesteckerDebbie RombautChantal AndersElke ClicqueMarnik VuylstekeTom Jacobs

A1 Journal Article in PLANT JOURNAL

Leveraging a self-cleaving peptide for tailored control in proximity labeling proteomics

Louis DelhayeGeorgios MoschonasDaria FijalkowskaAnnick VerheeDelphine De SutterTessa Van de SteeneMargaux De MeyerHanna GrzesikLaura Van MoortelKarolien De Bosscher et al.

A2 Journal Article in CELL REPORTS METHODS

Orthogonal LoxPsym sites allow multiplexed site-specific recombination in prokaryotic and eukaryotic hosts

Charlotte CautereelsJolien SmetsJonas De SaegerLloyd CoolYanmei ZhuAnna ZimmermannJan SteenselsAnton GorkovskiyTom JacobsKevin J. Verstrepen

A1 Journal Article in NATURE COMMUNICATIONS

Phosphoproteome analyses pinpoint the F‐box protein SLOW MOTION as a regulator of warm temperature‐mediated hypocotyl growth in Arabidopsis

Shanshuo ZhuLixia PanLam Dai VuXiangyu XuBeatriz Orosa‐PuenteTingting ZhuPia NeytBrigitte Van De CotteTom JacobsJoshua M. Gendron et al.

A1 Journal Article in NEW PHYTOLOGIST

2023

BREEDIT : a multiplex genome editing strategy to improve complex quantitative traits in maize

Christian LorenzoKevin DebrayDenia HerweghWard DeveltereLennert ImpensDries SchaumontWout VandeputteStijn AesaertGriet CoussensYara De Boe et al.

A1 Journal Article in PLANT CELL

Combining multiplex gene editing and doubled haploid technology in maize

Lennert ImpensChristian LorenzoWout VandeputtePieter WytynckKevin DebrayJari HaeghebaertDenia HerweghTom JacobsTom RuttinkHilde Nelissen et al.

A1 Journal Article in NEW PHYTOLOGIST

Identification and characterization of CYP71 subclade cytochrome P450 enzymes involved in the biosynthesis of bitterness compounds in Cichorium intybus

Charlotte De BruynTom RuttinkElia LacchiniStephane RombautsAnnelies Haegemanellen de keyserChristof Van PouckeSandrien DesmetTom JacobsTom Eeckhaut et al.

A1 Journal Article in FRONTIERS IN PLANT SCIENCE

Multiplex CRISPR/Cas9 editing of susceptibility genes for durable resistance to late blight disease in potato

Joanna LukasiewiczTom JacobsTom RuttinkTom EeckhautFrank Van Breusegem

SMAP design : a multiplex PCR amplicon and gRNA design tool to screen for natural and CRISPR-induced genetic variation

Ward DeveltereEvelien WaegneerKevin DebrayJonas De SaegerSabine Van GlabekeSteven MaereTom RuttinkTom Jacobs

A1 Journal Article in NUCLEIC ACIDS RESEARCH

Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform

Christophe GaillochetAlexandra Pena FernandezVera GoossensKatelijn D’HalluinAndrzej DrozdzeckiMyriam ShafieJulie Van DuyseGert Van IsterdaelCamila GonzalezMattias Vermeersch et al.

A1 Journal Article in GENOME BIOLOGY

Towards overcoming genetic redundancy and lethality : design and implementation of novel CRISPR tools in plant programmed cell death research

Marie PfeifferMoritz NowackTom Jacobs

Ulva : an emerging green seaweed model for systems biology

Jonas BlommeThomas WichardTom JacobsOlivier De Clerck

A1 Journal Article in JOURNAL OF PHYCOLOGY

2022

A simple and low‐tech heat‐shock method to increase genome editing efficiency in plants

Jonas BlommeJúlia ArraizaWard DeveltereTom Jacobs

A2 Journal Article in CURRENT PROTOCOLS

Conditional and tissue-specific approaches to dissect essential mechanisms in plant development

Marie PfeifferJoanna WinklerDaniël Van DammeTom JacobsMoritz Nowack

A1 Journal Article in CURRENT OPINION IN PLANT BIOLOGY

Mini-review : transgenerational CRISPR/Cas9 gene editing in plants

Lennert ImpensTom JacobsHilde NelissenDirk InzéLaurens Pauwels

A2 Journal Article in FRONTIERS IN GENOME EDITING

Mutually opposing activity of PIN7 splicing isoforms is required for auxin‐mediated tropic responses in Arabidopsis thaliana

Ivan KashkanMónika HrtyanKatarzyna RetzerJana HumpolíčkováAswathy JayasreeRoberta FilepováZuzana VondrákováSibu SimonDebbie RombautTom Jacobs et al.

A1 Journal Article in NEW PHYTOLOGIST

Optimized transformation and gene editing of the B104 public maize inbred by improved tissue culture and use of morphogenic regulators

Stijn AesaertLennert ImpensGriet CoussensEls Van LerbergeRudy VanderhaeghenLaurence DesmetYasmine VanhevelShari BossuytAngeline Ndele WambuaMaria Van Lijsebettens et al.

A1 Journal Article in FRONTIERS IN PLANT SCIENCE

Protoplast preparation and fluorescence-activated cell for the evaluation of targeted mutagenesis in plant cells

Ward DecaesteckerNorbert BollierRafael Andrade BuonoMoritz NowackTom Jacobs

Bookchapter in Protoplast technology

ROPGAP-dependent interaction between brassinosteroid and ROP2-GTPase signaling controls pavement cell shape in Arabidopsis

Cheng ZhangTheresa LausterWenxin TangAnaxi HoubaertShanshuo ZhuDominique EeckhoutIve De SmetGeert De JaegerTom JacobsTongda Xu et al.

A1 Journal Article in CURRENT BIOLOGY

SMAP design : a multiplex PCR amplicon and gRNA design tool to screen for natural and CRISPR-induced genetic variation

Ward DeveltereEvelien WaegneerKevin DebraySabine Van GlabekeSteven MaereTom RuttinkTom Jacobs

Simple and efficient modification of Golden Gate design standards and parts using oligo stitching

Jonas De SaegerMattias VermeerschChristophe GaillochetTom Jacobs

A1 Journal Article in ACS SYNTHETIC BIOLOGY

The heat is on : a simple method to increase genome editing efficiency in plants

Jonas BlommeWard DeveltereAyse KöseJúlia ArraizaChristophe BrugmansJessica Jaraba WallaceWard DecaesteckerDebbie RombautAlexandra BaekelandtÁlvaro Daniel Fernández Fernández et al.

A1 Journal Article in BMC PLANT BIOLOGY

2021

A molecular toolkit for the green seaweed Ulva mutabilis

Jonas BlommeXiaojie LiuTom JacobsOlivier De Clerck

A1 Journal Article in PLANT PHYSIOLOGY

CRISPR screens in plants : approaches, guidelines, and future prospects

Christophe GaillochetWard DeveltereTom Jacobs

A1 Journal Article in PLANT CELL

Efficient CRISPR-mediated base editing in Agrobacterium spp.

Savio D. RodriguesMansour KarimiLennert ImpensEls Van LerbergeGriet CoussensStijn AesaertDebbie RombautDominique HoltappelsHeba M. M. IbrahimMarc Van Montagu et al.

A1 Journal Article in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient simultaneous mutagenesis of multiple genes in specific plant tissues by multiplex CRISPR

Norbert BollierRafael Andrade BuonoTom JacobsMoritz Nowack

A1 Journal Article in PLANT BIOTECHNOLOGY JOURNAL

GoldenGateway : a DNA assembly method for plant biotechnology

Mansour KarimiTom Jacobs

Editorial material

Optimization of CRISPR/Cas9 genome editing and the development of tools for tissue-specific knock-out, gene targeting and prime editing in Arabidopsis thaliana

Ward DecaesteckerMoritz NowackTom Jacobs

Voices of biotech research

Nasim AnnabiMatthew BakerAlistair BoettigerDebojyoti ChakrabortyYvonne ChenKizzmekia S. CorbettBruno CorreiaJames DahlmanTulio de OliveiraAli Ertuerk et al.

Editorial material

2020

CRISPR-Cas-mediated gene knockout in tomato

Gwen SwinnenTom JacobsLaurens PauwelsAlain Goossens

Bookchapter in Plant and food carotenoids : methods and protocols

Establishment of CRISPR/Cas9 genome editing in witloof (Cichorium intybus var. foliosum)

Charlotte De BruynTom RuttinkTom EeckhautTom JacobsEllen De KeyserAlain GoossensKatrijn Van Laere

A2 Journal Article in FRONTIERS IN GENOME EDITING

2019

CRISPR-TSKO : a technique for efficient mutagenesis in specific cell types, tissues, or organs in Arabidopsis

Ward DecaesteckerRafael Andrade BuonoMarie PfeifferNick VangheluweJoris JourquinMansour KarimiGert Van IsterdaelTom BeeckmanMoritz NowackTom Jacobs

A1 Journal Article in PLANT CELL

DOF2.1 controls cytokinin-dependent vascular cell proliferation downstream of TMO5/LHW

Wouter SmetIris SevilemMaria Angels de Luis BalaguerBrecht WybouwEliana MorShunsuke MiyashimaBernhard BlobPawel RoszakTom JacobsMark Boekschoten et al.

A1 Journal Article in CURRENT BIOLOGY

Genome-editing based engineering of CESA3 dual cellulose-inhibitor resistant plants

Zhubing HuTeng ZhangDebbie RombautWard DecaesteckerAiming XingSam D'HaeyerRené HöferIlse VercauterenMansour KarimiTom Jacobs et al.

A1 Journal Article in PLANT PHYSIOLOGY

2018

POLAR-guided signalling complex assembly and localization drive asymmetric cell division

Anaxi HoubaertCheng ZhangManish TiwariKun WangAlberto de Marcos SerranoDaniel SavatinMounashree J UrsMiroslava ZhiponovaGustavo GudesblatIsabelle Vanhoutte et al.

A1 Journal Article in NATURE

2017

Generation of a collection of mutant tomato lines using pooled CRISPR libraries

Tom JacobsNing ZhangDhruv PatelGregory B Martin

A1 Journal Article in PLANT PHYSIOLOGY

2016

A transgenic, visual screenable marker for soybean seeds

Lisa KanizayTom JacobsC Nathan Hancock

A1 Journal Article in TRANSGENIC RESEARCH

High-throughput CRISPR vector construction and characterization of DNA modifications by generation of tomato hairy roots

Tom JacobsGregory B Martin

A1 Journal Article in JOVE-JOURNAL OF VISUALIZED EXPERIMENTS

Simple gene silencing using the trans-acting siRNA pathway

Tom JacobsNoah J LawlerPeter R LaFayetteLila O VodkinWayne A Parrott

A1 Journal Article in PLANT BIOTECHNOLOGY JOURNAL

2015

Exploiting SNPs for biallelic CRISPR mutations in the outcrossing woody perennial Populus reveals 4-coumarate : CoA ligase specificity and redundancy

Xiaohong ZhouTom JacobsLiang-Jiao XueScott A HardingChung-Jui Tsai

A1 Journal Article in NEW PHYTOLOGIST

HtStuf: high-throughput sequencing to locate unknown DNA junction fragments

Lisa B KanizayTom JacobsKevin GillespieJade A NewsomeBrittany N SpaidWayne A Parrott

A1 Journal Article in PLANT GENOME

Targeted genome modifications in soybean with CRISPR/Cas9

Tom JacobsPeter R LaFayetteRobert J SchmitzWayne A Parrott

A1 Journal Article in BMC BIOTECHNOLOGY