Research Explorer

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Researcher

Stefaan De Koker

Publications & Research Data

Awards & Distinctions

67 Results

2020

Imidazoquinoline-conjugated degradable coacervate conjugate for local cancer immunotherapy

Hui LiSimon Van HerckYongjun LiuYanyun HaoXiaochu DingLutz NuhnZifu ZhongFrancis CombesNiek SandersStefan Lienenklaus et al.

A1 Journal Article in ACS BIOMATERIALS SCIENCE & ENGINEERING

The opposing effect of type I IFN on the T cell response by non-modified mRNA-lipoplex vaccines Is determined by the route of administration

Lien Van HoeckeKenny RooseMarlies BallegeerZifu ZhongNiek SandersStefaan De KokerXavier SaelensSandra Van Lint

A1 Journal Article in MOLECULAR THERAPY-NUCLEIC ACIDS

2018

Delivering type I interferon to dendritic cells empowers tumor eradication and immune combination treatments

Anje CauwelsSandra Van LintFranciane PaulGenevieve GarcinStefaan De KokerAlexander Van ParysThomas WüestSarah GerloJosé Van Der HeydenYan Bordat et al.

A1 Journal Article in CANCER RESEARCH

Lymph-node-targeted immune activation by engineered block copolymer amphiphiles-TLR7/8 agonist conjugates

Simon Van HerckKim DeswarteLutz NuhnZifu ZhongJoão Portela CataniYupeng LiNiek SandersStefan LienenklausStefaan De KokerBart Lambrecht et al.

A1 Journal Article in JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Nanoparticle-conjugate TLR7/8 agonist localized immunotherapy provokes safe antitumoral responses

Lutz NuhnStefaan De KokerSandra Van LintZifu ZhongJoão Portela CataniFrancis CombesKim DeswarteYupeng LiBart LambrechtStefan Lienenklaus et al.

A1 Journal Article in ADVANCED MATERIALS

Post-PEGylated and crosslinked polymeric ssRNA nanocomplexes as adjuvants targeting lymph nodes with increased cytolytic T cell inducing properties

Bo LouAns De BeuckelaerGeorge DakwarKatrien RemautJohan GrootenKevin BraeckmansBruno De GeestEnrico MastrobattistaStefaan De KokerWim E. Hennink

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Potent anti-viral vaccine adjuvant based on pH-degradable nanogels with covalently linked small molecule imidazoquinoline TLR7/8 agonist

Lutz NuhnLien Van HoeckeKim DeswarteBert SchepensYupeng LiBart LambrechtStefaan De KokerSunil A DavidXavier SaelensBruno De Geest

A1 Journal Article in BIOMATERIALS

Treatment with mRNA coding for the necroptosis mediator MLKL induces antitumor immunity directed against neo-epitopes

Lien Van HoeckeSandra Van LintKenny RooseAlexander Van ParysPeter VandenabeeleJohan GrootenJan TavernierStefaan De KokerXavier Saelens

A1 Journal Article in NATURE COMMUNICATIONS

2017

Arginine-rich peptide-based mRNA nanocomplexes efficiently instigate cytotoxic T cell immunity dependent on the amphipathic organization of the peptide

Vimal Kumar UdhayakumarAns De BeuckelaerJoanne McCaffreyCian M McCruddenJonathan L KirschmanDaryll VanoverLien Van HoeckeKenny RooseKim DeswarteBruno De Geest et al.

A1 Journal Article in ADVANCED HEALTHCARE MATERIALS

Development and validation of synthetic-virus like vaccines

Vimal Kumar UdhayakumarJohan GrootenStefaan De Koker

Inflammatory monocytes regulate Th1 oriented immunity to CpG adjuvanted protein vaccines through production of IL-12

Stefaan De KokerLien Van HoeckeAns De BeuckelaerKenny RooseKim DeswarteMonique WillartPieter BogaertThomas NaessensBruno De GeestXavier Saelens et al.

A1 Journal Article in SCIENTIFIC REPORTS

Species-specific immunity to Helicobacter suis

Iris BosschemBram FlahouKim Van DeunStefaan De KokerJiri VolfAnnemieke SmetRichard DucatelleBert DevriendtFreddy Haesebrouck

A1 Journal Article in HELICOBACTER

Type I interferons modulate CD8⁺ T cell immunity to mRNA vaccines

Ans De BeuckelaerJohan GrootenStefaan De Koker

A1 Journal Article in TRENDS IN MOLECULAR MEDICINE

2016

Engineering polymer hydrogel nanoparticles for lymph node-targeted delivery

Stefaan De KokerJivei CuiNane VanparijsLorenzo AlbertazziJohan GrootenFrank CarusoBruno De Geest

A1 Journal Article in ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

GM-CSF treatment prevents respiratory syncytial virus– induced pulmonary exacerbation responses in postallergic mice by stimulating alveolar macrophage maturation

Thomas NaessensBert SchepensMuriel SmetCharlotte PollardLien Van HoeckeAns De BeuckelaerMonique WillartBart LambrechtStefaan De KokerXavier Saelens et al.

A1 Journal Article in JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

mRNA-based vaccines to elicit CD8⁺ T cell immunity

Ans De BeuckelaerJohan GrootenStefaan De Koker

Mycobacterium tuberculosis-associated synthetic mycolates differentially exert immune stimulatory adjuvant activity

Muriel SmetCharlotte PollardAns De BeuckelaerLien Van HoeckeSeppe Vander BekenStefaan De KokerJuma'a R Al DulayymiKris HuygenJan VerschoorMark S Baird et al.

A1 Journal Article in EUROPEAN JOURNAL OF IMMUNOLOGY

pH-degradable imidazoquinoline-ligated nanogels for lymph node-focused immune activation

Lutz NuhnNane VanparijsAns De BeuckelaerLien LybaertGlenn VerstraeteKim DeswarteStefan LienenklausNikunj M ShuklaAlex CD SalyerBart Lambrecht et al.

A1 Journal Article in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

pH-Degradable mannosylated nanogels for dendritic cell targeting

Ruben De CoenNane VanparijsMartijn RisseeuwLien LybaertBenoit LouageStefaan De KokerVimal Kumar UdhayakumarJohan GrootenLeeanne TaylorNeil Ayres et al.

A1 Journal Article in BIOMACROMOLECULES

Spontaneous protein adsorption on graphene oxide nanosheets allowing efficient intracellular vaccine protein delivery

Hui LiKaat FierensZhiyue ZhangNane VanparijsMartijn SchuijsKatleen Van SteendamNatalia Feiner GraciaRiet De RyckeThomas De BeerAns De Beuckelaer et al.

A1 Journal Article in ACS APPLIED MATERIALS & INTERFACES

Type I interferons interfere with the capacity of mRNA lipoplex vaccines to elicit cytolytic T cell responses

Ans De BeuckelaerCharlotte PollardSandra Van LintKenny RooseLien Van HoeckeTom NaessensVimal Kumar UdhayakumarMuriel SmetNiek SandersStefan Lienenklaus et al.

A1 Journal Article in MOLECULAR THERAPY

Vaccination with necroptotic cancer cells induces efficient anti-tumor immunity

Tania Love AaesAgnieszka KaczmarekTinneke DelvaeyeBram De CraeneStefaan De KokerLiesbeth HeyndrickxIris DelrueJoachim TaminauBartosz WiernickiPhilippe De Groote et al.

A1 Journal Article in CELL REPORTS

2015

Hybrid pulmonary surfactant-coated nanogels mediate efficient in vivo delivery of siRNA to murine alveolar macrophages

Lynn De BackerThomas NaessensStefaan De KokerElisa ZagatoJo DemeesterJohan GrootenStefaan De SmedtKoen Raemdonck

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

The site of administration influences both the type and the magnitude of the immune response induced by DNA vaccine electroporation

Gaëlle VandermeulenKevin VanvarenbergAns De BeuckelaerStefaan De KokerLaure LambrichtCatherine UyttenhoveAnca ReschnerAlain VanderplasschenJohan GrootenVéronique Préat

A1 Journal Article in VACCINE

2014

Bio-hybrid tumor cell-templated capsules: a generic formulation strategy for tumor associated antigens in view of immune therapy

Lien LybaertElly De VlieghereRiet De RyckeNane VanparijsOlivier De WeverStefaan De KokerBruno De Geest

A1 Journal Article in ADVANCED FUNCTIONAL MATERIALS

Boosting in planta production of antigens derived from the porcine reproductive and respiratory syndrome virus (PRRSV) and subsequent evaluation of their immunogenicity

Robin PironStefaan De KokerAnnelies De PaepeJulie GoossensJohan GrootenHans NauwynckAnna Depicker

A1 Journal Article in PLOS ONE

High efficiency cell-specific targeting of cytokine activity

Geneviève GarcinFranciane PaulMarkus StaufenbielYann BordatJosé Van Der HeydenStephan WilmesGuillaume CartronFlorence ApparaillyStefaan De KokerJacob Piehler et al.

A1 Journal Article in NATURE COMMUNICATIONS

Just spray it: LbL assembly enters a new age

Marijke DierendonckStefaan De KokerRiet De RyckeBruno De Geest

A1 Journal Article in SOFT MATTER

Nanoporous hydrogen bonded polymeric microparticles: facile and economic production of cross presentation promoting vaccine carriers

Marijke DierendonckKaat FierensRiet De RyckeLien LybaertSamarendra MajiZhiyue ZhangQilu ZhangRichard HoogenboomBart LambrechtJohan Grooten et al.

A1 Journal Article in ADVANCED FUNCTIONAL MATERIALS

Nanoporous microparticles : towards a generic vaccine formulation platform

Marijke DierendonckBruno De GeestStefaan De Koker

Nanoporous polyelectrolyte vaccine microcarriers: a formulation platform for enhancing humoral and cellular immune responses

Stefaan De KokerKaat FierensMarijke DierendonckRiet De RyckeBart LambrechtJohan GrootenJean Paul RemonBruno De Geest

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

2013

Challenges and advances towards the rational design of mRNA vaccines

Charlotte PollardStefaan De KokerXavier SaelensGuido VanhamJohan Grooten

A1 Journal Article in TRENDS IN MOLECULAR MEDICINE

Evaluation of CD8⁺ T cell responses upon mRNA vaccination and the implication of type I IFN signaling

Charlotte PollardJohan GrootenStefaan De KokerGuido Vanham

High efficiency targeting of interferon α activity

Geneviève GarcinFranciane PaulMarkus StaufenbielYann BordatJosé Van Der HeydenStephan WilmesGuillaume CartronFlorence ApparaillyStefaan De KokerJacob Piehler et al.

Lipoplexes carrying mRNA encoding Gag protein modulate dendritic cells to stimulate HIV-specific immune responses

Winni De HaesJoanna RejmanCharlotte PollardCéline MerlinMarc VekemansEric FlorenceStefaan De SmedtJohan GrootenGuido VanhamStefaan De Koker et al.

A1 Journal Article in NANOMEDICINE

Magnetically engineered microcapsules as intracellular anchors for remote control over cellular mobility

Anton M PavlovBruno De GeestBenoit LouageLien LybaertStefaan De KokerZdenek KoudelkaAndrei V SapelkinGleb B Sukhorukov

A1 Journal Article in ADVANCED MATERIALS

One-step spray-dried polyelectrolyte microparticles enhance the antigen cross-presentation capacity of porcine dendritic cells

Bert DevriendtKim BaertMarijke DierendonckHerman FavoreelStefaan De KokerJean Paul RemonBruno De GeestEric Cox

A1 Journal Article in EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

Systematic processing of beta-tricalcium phosphate for efficient protein loading and in vitro analysis of antigen uptake

Vinayaraj Ozhukil KollathBruno De GeestSteven MullensStefaan De KokerJan LuytenRosita PersoonsKarl TrainaJean Paul RemonRudi Cloots

A1 Journal Article in ADVANCED ENGINEERING MATERIALS

Type I IFN counteracts the induction of antigen-specific immune responses by lipid-based delivery of mRNA vaccines

Charlotte PollardJoanna RejmanWinni De HaesBernard VerrierEllen Van GulckThomas NaessensStefaan De SmedtPieter BogaertJohan GrootenGuido Vanham et al.

A1 Journal Article in MOLECULAR THERAPY

2012

Innate imprinting of murine resident alveolar macrophages by allergic bronchial inflammation causes a switch from hypoinflammatory to hyperinflammatory reactivity

Thomas NaessensSeppe Vander BekenPieter BogaertNico Van RooijenStefan LienenklausSiegfried WeissStefaan De KokerJohan Grooten

A1 Journal Article in AMERICAN JOURNAL OF PATHOLOGY

Interaction between polymeric multilayer capsules and immune cells

Marijke DierendonckStefaan De KokerChris VervaetJean Paul RemonBruno De Geest

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Polyelectrolyte LbL microcapsules versus PLGA microparticles for immunization with a protein antigen

Marie-Luce De TemmermanJoanna RejmanRoosmarijn VandenbrouckeStefaan De KokerClaude LibertJohan GrootenJo DemeesterBruno GanderStefaan De Smedt

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Polymeric multilayer capsule-mediated vaccination induces protective immunity against cancer and viral infection

Bruno De GeestMonique WillartHamida HammadBart LambrechtCharlotte PollardPieter BogaertMarina De FiletteXavier SaelensChris VervaetJean Paul Remon et al.

A1 Journal Article in ACS NANO

Polymeric multilayer capsules for drug delivery

Stefaan De KokerRichard HoogenboomBruno De Geest

A1 Journal Article in CHEMICAL SOCIETY REVIEWS

Surface-engineered polyelectrolyte multilayer capsules: synthetic vaccines mimicking microbial structure and function

Bruno De GeestMonique WillartBart LambrechtCharlotte PollardChris VervaetJean Paul RemonJohan GrootenStefaan De Koker

A1 Journal Article in ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

2011

Designing polymeric particles for antigen delivery

Stefaan De KokerBart LambrechtMonique WillartYvette van KooykJohan GrootenChris VervaetJean Paul RemonBruno De Geest

A1 Journal Article in CHEMICAL SOCIETY REVIEWS

Hydrophobic shell loading of biopolyelectrolyte capsules

Di CuiJing JingThomas BoudouIsabelle Pignot-PaintrandStefaan De KokerBruno De GeestCatherine PicartRachel Auzely-Velty

A1 Journal Article in ADVANCED MATERIALS

Inflammatory signatures for eosinophilic vs. neutrophilic allergic pulmonary inflammation reveal critical regulatory checkpoints

Pieter BogaertThomas NaessensStefaan De KokerBenoit HennuyJonathan HachaMuriel SmetDidier CataldoEmmanuel Di ValentinJacques PietteKurt Tournoy et al.

A1 Journal Article in AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY

Mucosal irritation potential of polyelectrolyte multilayer capsules

Liesbeth De CockJoke LenoirStefaan De KokerVincent VermeerschAndre SkirtachPeter DubruelEls AdriaensChris VervaetJean Paul RemonBruno De Geest

A1 Journal Article in BIOMATERIALS

Polymeric multilayer capsules delivering biotherapeutics

Stefaan De KokerLiesbeth De CockPilar Rivera-GilWolfgang J ParakRachel Auzély VeltyChris VervaetJean Paul RemonJohan GrootenBruno De Geest

A1 Journal Article in ADVANCED DRUG DELIVERY REVIEWS

Single-step formation of degradable intracellular biomolecule microreactors

Marijke DierendonckStefaan De KokerRiet De RyckePieter BogaertJohan GrootenChris VervaetJean Paul RemonBruno De Geest

A1 Journal Article in ACS NANO

2010

A single step process for the synthesis of antigen laden thermosensitive microparticles

Bruno De GeestStefaan De KokerYves GonnissenLiesbeth De CockJohan GrootenJean Paul RemonChris Vervaet

A1 Journal Article in SOFT MATTER

Biodegradable polyelectrolyte microcapsules: antigen delivery tools with Th17 skewing activity after pulmonary delivery

Stefaan De KokerThomas NaessensBruno De GeestPieter BogaertJo DemeesterStefaan De SmedtJohan Grooten

A1 Journal Article in JOURNAL OF IMMUNOLOGY

Facile two-step synthesis of porous antigen-loaded degradable polyelectrolyte microspheres

Marijke DierendonckStefaan De KokerClaude CuvelierJohan GrootenChris VervaetJean Paul RemonBruno De Geest

A1 Journal Article in ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Layer-by-layer incorporation of growth factors in decellularized aortic heart valve leaflets

Liesbeth De CockStefaan De KokerFilip De VosChris VervaetJean Paul RemonBruno De Geest

A1 Journal Article in BIOMACROMOLECULES

Polyelectrolyte capsules-containing HIV-1 p24 and poly I:C modulate dendritic cells to stimulate HIV-1-specific immune responses

Winni De HaesStefaan De KokerCharlotte PollardDerek AtkinsonErika VliegheJessy HosteJoanna RejmanStefaan De SmedtJohan GrootenGuido Vanham et al.

A1 Journal Article in MOLECULAR THERAPY

Polymeric multilayer capsules in drug delivery

Liesbeth De CockStefaan De KokerBruno De GeestJohan GrootenChris VervaetJean Paul RemonGleb B SukhorukovMaria N Antipina

A1 Journal Article in ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Self-exploding capsules

Bruno De GeestStefaan De KokerJo DemeesterStefaan De SmedtWim E Hennink

A1 Journal Article in POLYMER CHEMISTRY

2009

Intracellular processing of proteins mediated by biodegradable polyelectrolyte capsules

Pilar Rivera-GilStefaan De KokerBruno De GeestWolfgang J Parak

A1 Journal Article in NANO LETTERS

Polyelectrolyte Microcapsules as Antigen Delivery Vehicles To Dendritic Cells: Uptake, Processing, and Cross-Presentation of Encapsulated Antigens

Stefaan De KokerBruno De GeestSatwinder SinghRiet De RyckeThomas NaessensYvette Van KooykJo DemeesterStefaan De SmedtJohan Grooten

A1 Journal Article in ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Polyelectrolyte microcapsules for biomedical applications

Bruno De GeestStefaan De KokerGB SukhorukovO KreftWJ ParakAndre SkirtachJo DemeesterStefaan De SmedtWim E Hennink

A1 Journal Article in Soft Matter

Pulsed in vitro release and in vivo behavior of exploding microcapsules

Bruno De GeestStefaan De KokerJo DemeesterStefaan De SmedtWE Hennink

A1 Journal Article in Journal of Controlled Release

2008

Polyelectrolyte microcapsules for antigen delivery

Stefaan De KokerJohan GrootenStefaan De SmedtNico Mertens

Self exploding microcapsules

Bruno De GeestStefaan De KokerJo DemeesterStefaan De SmedtWim E Hennink

Self-Exploding Beads Releasing Microcarriers

Bruno De GeestStefaan De KokerKristof ImmesoeteJo DemeesterStefaan De SmedtWE HENNINK

A1 Journal Article in ADVANCED MATERIALS

2007

In vivo cellular uptake, degradation, and biocompatibility of polyelectrolyte microcapsules

Stefaan De KokerBruno De GeestClaude CuvelierLiesbeth FerdinandeWies DeckersWE HENNINKStefaan De SmedtNico Mertens

A1 Journal Article in ADVANCED FUNCTIONAL MATERIALS

2004

New strategies in polypeptide and antibody synthesis: An overview

Nico MertensF DEVOSJ LEls Van DeynseAn WillemsSteve SchoonoogheIngrid BurvenichStefaan De KokerDominique VliegheJohan Grooten et al.

A1 Journal Article in CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS