Research Explorer

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Researcher

Ruben Vanholme

Awards & Distinctions

77 Results

2023

Downregulation of barley ferulate 5-hydroxylase dramatically alters straw lignin structure without impact on mechanical properties

Reza ShafieiMatthew HooperChristopher McClellanHelena OakeyJennifer StephensCatherine LapierreYukiko TsujiGeert GoeminneRuben VanholmeWout Boerjan et al.

A1 Journal Article in FRONTIERS IN PLANT SCIENCE

From lab to field : improving biomass processability of maize using genome editing

Yasmine VanhevelWout BoerjanRuben Vanholme

Genetic approaches to improve lignocellulosic biomass yield and processing efficiency in Arabidopsis and poplar

Marlies WoutersWout BoerjanRuben Vanholme

Hydroxycinnamaldehyde-derived benzofuran components in lignins

Koichi YoshiokaHoon KimFachuang LuNette De RidderRuben VanholmeShinya KajitaWout BoerjanJohn Ralph

U Journal Article in Plant Physiology

Microparticle-mediated CRISPR DNA delivery for genome editing in poplar

Lennart HoengenaertJan Van DoorsselaereRuben VanholmeWout Boerjan

U Journal Article in Frontiers in Plant Science

QT–GWAS : a novel method for unveiling biosynthetic loci affecting qualitative metabolic traits

Marlies BrouckaertMeng PengRené HöferIlias El HouariChiarina DarrahVeronique StormeYvan SaeysRuben VanholmeGeert GoeminneVitaliy I. Timokhin et al.

A1 Journal Article in MOLECULAR PLANT

Reassessing the claimed cytokinin-substituting activity of dehydrodiconiferyl alcohol glucoside

Klaas WitvrouwHoon KimRuben VanholmeGeert GoeminneJohn RalphWout BoerjanBartel Vanholme

A1 Journal Article in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Suppression of the Arabidopsis cinnamoyl-CoA reductase 1-6 intronic T-DNA mutation by epigenetic modification

Marlies WoutersHéloïse BastiaanseStephane RombautsLisanne de VriesTim De PooterMojca StrazisarGodfrey NeutelingsRuben VanholmeWout Boerjan

A1 Journal Article in PLANT PHYSIOLOGY

2022

Engineering Curcumin Biosynthesis in Poplar Affects Lignification and Biomass Yield

Barbara De MeesterPaula Oyarce SgroRuben VanholmeRebecca Van AckerYukiko TsujiThijs VangeelSander Van den BoschJan Van DoorsselaereBert SelsJohn Ralph et al.

A1 Journal Article in FRONTIERS IN PLANT SCIENCE

Lignin engineering in forest trees : from gene discovery to field trials

Barbara De MeesterRuben VanholmeThatiane Rodrigues MotaWout Boerjan

A1 Journal Article in PLANT COMMUNICATIONS

Overexpression of the scopoletin biosynthetic pathway enhances lignocellulosic biomass processing

Lennart HoengenaertMarlies WoutersHoon KimBarbara De MeesterKris MorreelSteven VandersyppeJacob PollierSandrien DesmetGeert GoeminneJohn Ralph et al.

A1 Journal Article in SCIENCE ADVANCES

Rerouting of the lignin biosynthetic pathway by inhibition of cytosolic shikimate recycling in transgenic hybrid aspen

Shi HuNaofumi KamimuraShingo SakamotoSoichiro NaganoNaoki TakataSarah LiuGeert GoeminneRuben VanholmeMikiko UesugiMasanobu Yamamoto et al.

A1 Journal Article in PLANT JOURNAL

Synthesis of hydroxycinnamoyl shikimates and their role in monolignol biosynthesis

Dharshana PadmakshanVitaliy, I TimokhinFachuang LuPaul F. SchatzRuben VanholmeWout BoerjanJohn Ralph

A1 Journal Article in HOLZFORSCHUNG

2021

CRISPR‐Cas9 editing of Caffeoyl Shikimate Esterase 1 and 2 shows their importance and partial redundancy in lignification in Populus tremula x P. alba

Lisanne de VriesMarlies BrouckaertAlexandra Alvarenga ChanocaHoon KimMatthew R. RegnerVitaliy I. TimokhinYi SunBarbara De MeesterJan Van DoorsselaereGeert Goeminne et al.

A1 Journal Article in PLANT BIOTECHNOLOGY JOURNAL

Incorporation of catechyl monomers into lignins : lignification from the non-phenolic end via Diels–Alder cycloaddition?

Daisuke AndoFachuang LuHoon KimAlexis EugeneYuki TobimatsuRuben VanholmeThomas J. ElderWout BoerjanJohn Ralph

A1 Journal Article in GREEN CHEMISTRY

Maize specialized metabolome networks reveal organ-preferential mixed glycosides

Sandrien DesmetYvan SaeysKevin VerstaenRebecca DauweHoon KimClaudiu NiculaesAtsushi FukushimaGeert GoeminneRuben VanholmeJohn Ralph et al.

A1 Journal Article in COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL

Optimizing lignocellulosic biomass for the biorefinery and developing tools to facilitate the introduction of genome-engineered poplars into the field

Lennart HoengenaertWout BoerjanRuben Vanholme

Rewired phenolic metabolism and improved saccharification efficiency of a Zea mays cinnamyl alcohol dehydrogenase 2 (zmcad2) mutant

Xinyu LiuRebecca Van AckerWannes VoorendAndreas PallidisGeert GoeminneJacob PollierKris MorreelHoon KimHilde MuylleMickael Bosio et al.

A1 Journal Article in PLANT JOURNAL

Vessel‐ and ray‐specific monolignol biosynthesis as an approach to engineer fiber‐hypolignification and enhanced saccharification in poplar

Barbara De MeesterRuben VanholmeLisanne de VriesMarlies WoutersJan Van DoorsselaereWout Boerjan

A1 Journal Article in PLANT JOURNAL

2020

A century-old mystery unveiled : Sekizaisou is a natural lignin mutant

Masanobu YamamotoHirokazu TomiyamaAkio KoyamaHisato OkuizumiSarah LiuRuben VanholmeGeert GoeminneYuta HiraiHu ShiNuoendagula Nuoendagula et al.

A1 Journal Article in PLANT PHYSIOLOGY

Novel insights into phenylpropanoid biosynthesis by combining genetics and metabolomics

Xinyu LiuWout BoerjanRuben Vanholme

PIRIN2 suppresses S‐type lignin accumulation in a noncell‐autonomous manner in Arabidopsis xylem elements

Bo ZhangBernadette SztojkaSacha EscamezRuben VanholmeMattias HedenströmYin WangHalbay TurumtayAndrás GorzsásWout BoerjanHannele Tuominen

A1 Journal Article in NEW PHYTOLOGIST

Tailoring poplar lignin without yield penalty by combining a null and haploinsufficient CINNAMOYL-CoA REDUCTASE2 allele

Barbara De MeesterBárbara Loreto Madariaga CalderónLisanne de VriesJacob PollierGeert GoeminneJan Van DoorsselaereMingjie ChenJohn RalphRuben VanholmeWout Boerjan

A1 Journal Article in NATURE COMMUNICATIONS

Unraveling the function of genes involved in phenylpropanoid metabolism selected via coexpression and mGWAS in Arabidopsis

Jin LiWout BoerjanRuben Vanholme

2019

COSY catalyses trans-cis isomerization and lactonization in the biosynthesis of coumarins

Ruben VanholmeLisa SundinCarol SeetsoHoon KimXinyu LiuJin LiBarbara De MeesterLennart HoengenaertGeert GoeminneKris Morreel et al.

A1 Journal Article in NATURE PLANTS

Engineering lignin to support the bioeconomy

Marina de Lyra Soriano SalemeWout BoerjanRuben Vanholme

Introducing curcumin biosynthesis in Arabidopsis enhances lignocellulosic biomass processing

Paula Oyarce SgroBarbara De MeesterFernando Campos De Assis FonsecaLisanne de VriesGeert GoeminneAndreas PallidisRiet De RyckeYukiko TsujiYanding LiSander Van den Bosch et al.

A1 Journal Article in NATURE PLANTS

Lignin biosynthesis and its integration into metabolism

Ruben VanholmeBarbara De MeesterJohn RalphWout Boerjan

A1 Journal Article in CURRENT OPINION IN BIOTECHNOLOGY

Optimization of lignocellulosic biomass for the bio-refinery with Arabidopsis and poplar as model systems

Lisanne de VriesWout BoerjanRuben Vanholme

RNAi-suppression of barley caffeic acid O-methyltransferase modifies lignin despite redundancy in the gene family

Paul DalyChristopher McClellanMarta MalukHelena OakeyCatherine LapierreRobbie WaughJennifer StephensDavid MarshallAbdellah BarakateYukiko Tsuji et al.

A1 Journal Article in PLANT BIOTECHNOLOGY JOURNAL

2018

Application of Py-GC/MS coupled with PARAFAC2 and PLS-DA to study fast pyrolysis of genetically engineered poplars

Hilal Ezgi ToramanVictor AbrahamssonRuben VanholmeRebecca Van AckerFrederik RonsseGilles PilateWout BoerjanKevin Van GeemGuy Marin

A1 Journal Article in JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS

Optimization of poplar for the bio-based economy through tissue-specific lignification and the incorporation of alternative lignin monomers

Barbara De MeesterRuben VanholmeWout Boerjan

Stacking of a low-lignin trait with an increased guaiacyl and 5-hydroxyguaiacyl unit trait leads to additive and synergistic effects on saccharification efficiency in Arabidopsis thaliana

Lisanne de VriesRuben VanholmeRebecca Van AckerBarbara De MeesterLisa SundinWout Boerjan

A1 Journal Article in BIOTECHNOLOGY FOR BIOFUELS

The effect of altered lignin composition on mechanical properties of CINNAMYL ALCOHOL DEHYDROGENASE (CAD) deficient poplars

Merve OzparpucuNotburga GierlingerIngo BurgertRebecca Van AckerRuben VanholmeWout BoerjanGilles PilateAnnabelle DejardinMarkus Ruggeberg

A1 Journal Article in PLANTA

Vessel-specific reintroduction of CINNAMOYL-COA REDUCTASE1 (CCR1) in dwarfed ccr1 mutants restores vessel and xylary fiber integrity and increases biomass

Barbara De MeesterLisanne de VriesMerve ÖzparpucuNotburga GierlingerSander CorneillieAndreas PallidisGeert GoeminneKris MorreelMichiel De BruyneRiet De Rycke et al.

A1 Journal Article in PLANT PHYSIOLOGY

2017

Degradation of lignin β-aryl ether units in Arabidopsis thaliana expressing LigD, LigF and LigG from Sphingomonas paucimobilis SYK-6

Ewelina MnichRuben VanholmePaula Oyarce SgroSarah LiuFachuang LuGeert GoeminneBodil JørgensenMohammed S MotawieWout BoerjanJohn Ralph et al.

A1 Journal Article in PLANT BIOTECHNOLOGY JOURNAL

Different routes for conifer- and sinapaldehyde and higher saccharification upon deficiency in the dehydrogenase CAD1

Rebecca Van AckerAnnabelle DéjardinSandrien DesmetLennart HoengenaertRuben VanholmeKris MorreelFrançoise LauransHoon KimNicholas SantoroCliff Foster et al.

A1 Journal Article in PLANT PHYSIOLOGY

Silencing CAFFEOYL SHIKIMATE ESTERASE affects lignification and improves saccharification in poplar

Marina de Lyra Soriano SalemeIgor CesarinoLivia Vargas dos Santos FerreiraHoon KimRuben VanholmeGeert GoeminneRebecca Van AckerFernando Campos De Assis FonsecaAndreas PallidisWannes Voorend et al.

A1 Journal Article in PLANT PHYSIOLOGY

Silencing CHALCONE SYNTHASE in maize impedes the incorporation of tricin into lignin and increases lignin content

Nubia Barbosa EloyWannes VoorendWu LanMarina de Lyra Soriano SalemeIgor CesarinoRuben VanholmeRebecca SmithGeert GoeminneAndreas PallidisKris Morreel et al.

A1 Journal Article in PLANT PHYSIOLOGY

Unravelling the impact of lignin on cell wall mechanics : a comprehensive study on young poplar trees downregulated for CINNAMYL ALCOHOL DEHYDROGENASE (CAD)

Merve ÖzparpucuMarkus RüggebergNotburga GierlingerIgor CesarinoRuben VanholmeWout BoerjanIngo Burgert

A1 Journal Article in PLANT JOURNAL

2016

Chemical genetics uncovers novel inhibitors of lignification, including p-iodobenzoic acid targeting CINNAMATE-4-HYDROXYLASE

Dorien Van de WouwerRuben VanholmeRaphael DecouGeert GoeminneDominique AudenaertLong NguyenRené HöferEdouard PesquetBartel VanholmeWout Boerjan

A1 Journal Article in PLANT PHYSIOLOGY

Designer lignins: harnessing the plasticity of lignification

Yaseen MottiarRuben VanholmeWout BoerjanJohn RalphShawn D Mansfield

A1 Journal Article in CURRENT OPINION IN BIOTECHNOLOGY

Improving total saccharification yield of Arabidopsis plants by vessel-specific complementation of caffeoyl shikimate esterase (cse) mutants

Livia Vargas dos Santos FerreiraIgor CesarinoRuben VanholmeWannes VoorendMarina de Lyra Soriano SalemeKris MorreelWout Boerjan

A1 Journal Article in BIOTECHNOLOGY FOR BIOFUELS

Micropyrolysis of natural poplar mutants with altered p-hydroxyphenyl lignin content

Jop VercruysseAndries PoupaertRuben VanholmeCatherine BastienBartel VanholmeWout BoerjanWolter PrinsFrederik Ronsse

A1 Journal Article in JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS

Overexpression of GA20-OXIDASE1 impacts plant height, biomass allocation and saccharification efficiency in maize

Wannes VoorendHilde NelissenRuben VanholmeAlex De VliegherFrank Van BreusegemWout BoerjanIsabel Roldán-RuizHilde MuylleDirk Inzé

A1 Journal Article in PLANT BIOTECHNOLOGY JOURNAL

Potential of genetically engineered hybrid poplar for pyrolytic production of bio-based phenolic compounds

Hilal Ezgi ToramanRuben VanholmeEleonora BorénYumi VanwonterghemMarko DjokicGüray YildizFrederik RonsseWolter PrinsWout BoerjanKevin Van Geem et al.

A1 Journal Article in BIORESOURCE TECHNOLOGY

Saccharification protocol for small-scale lignocellulosic biomass samples to test processing of cellulose into glucose

Rebecca Van AckerRuben VanholmeKathleen PiensWout Boerjan

A2 Journal Article in BIO-PROTOCOL

The allelochemical MDCA inhibits lignification and affects auxin homeostasis

Ward SteenackersIgor CesarinoPetr KlimaMussa QuareshyRuben VanholmeSander CorneillieRobert KumpfDorien Van de WouwerKarin LjungGeert Goeminne et al.

A1 Journal Article in PLANT PHYSIOLOGY

The response of the root proteome to the synthetic strigolactone GR24 in Arabidopsis

Alan WaltonElisabeth StesGeert GoeminneLukas BraemMarnik VuylstekeCedrick MatthysCarolien De CuyperAn StaesJonathan VandenbusscheFrançois-Didier Boyer et al.

A1 Journal Article in MOLECULAR & CELLULAR PROTEOMICS

2015

Introduction of chemically labile substructures into Arabidopsis lignin through the use of LigD, the C-dehydrogenase from Sphingobium sp. strain SYK-6

Yukiko TsujiRuben VanholmeYuki TobimatsuYasuyuki IshikawaClifton E FosterNaofumi KamimuraShojiro HishiyamaSaki HashimotoAmiu ShinoHirofumi Hara et al.

A1 Journal Article in PLANT BIOTECHNOLOGY JOURNAL

Reductive lignocellulose fractionation into soluble lignin-derived phenolic monomers and dimers and processable carbohydrate pulps

S Van den BoschW SchutyserRuben VanholmeT DriessenSF KoelewijnT RendersBarbara De MeesterWJJ HuijgenW DehaenCM Courtin et al.

A1 Journal Article in ENERGY & ENVIRONMENTAL SCIENCE

2014

Accumulation of N-acetylglucosamine oligomers in the plant cell wall affects plant architecture in a dose-dependent and conditional manner

Bartel VanholmeRuben VanholmeHalbay TurumtayGeert GoeminneIgor CesarinoFlorence GoubetKris MorreelJorge RencoretVincent BuloneCortwa Hooijmaijers et al.

A1 Journal Article in PLANT PHYSIOLOGY

Fast pyrolysis of genetically modified biomass

Hilal Ezgi ToramanRuben VanholmeMarko DjokicGüray YildizFrederik RonsseWolter PrinsWout BoerjanKevin Van GeemGuy Marin

Mutation of the inducible ARABIDOPSIS THALIANA CYTOCHROME P450 REDUCTASE2 alters lignin composition and improves saccharification

Lisa SundinRuben VanholmeJan GeerinckGeert GoeminneRené HöferHoon KimJohn RalphWout Boerjan

A1 Journal Article in PLANT PHYSIOLOGY

Profiling the thermochemical degradation behavior of natural low HCT poplar mutants using micropyrolysis

Jop VercruysseRuben VanholmeBartel VanholmeWout BoerjanWolter PrinsFrederik Ronsse

Side by side comparison of chemical compounds generated by aqueous pretreatments of maize stover, miscanthus and sugarcane bagasse

Leonardo D GómezRuben VanholmeSusannah BirdGeert GoeminneLuisa M TrindadeIgor PolikarpovRachael SimisterKris MorreelWout BoerjanSimon J McQueen-Mason

A1 Journal Article in BIOENERGY RESEARCH

Systematic structural characterization of metabolites in Arabidopsis via candidate substrate-product pair networks

Kris MorreelYvan SaeysOana DimaFachuang LuYves Van de PeerRuben VanholmeJohn RalphBartel VanholmeWout Boerjan

A1 Journal Article in PLANT CELL

The role of the secondary cell walls in plant resistance to pathogens

Eva MiedesRuben VanholmeWout BoerjanAntonio Molina

A1 Journal Article in FRONTIERS IN PLANT SCIENCE

Transcript and metabolite profiling for the evaluation of tobacco tree and poplar as feedstock for the bio-based industry

Colin RuprechtTakayuki TohgeAlisdair FernieCara L MortimerAmanda KozloPaul D FraserNorma FunkeIgor CesarinoRuben VanholmeWout Boerjan et al.

A1 Journal Article in JOURNAL OF VISUALIZED EXPERIMENTS

2013

Biomass reaction engineering driving genetic modification

Kevin Van GeemHilal Ezgi ToramanMichiel SchietseEleonora BorénRuben VanholmeLorenz GerberMarko DjokicGüray YildizFrederik RonsseWolter Prins et al.

Breeding with rare defective alleles (BRDA): a natural Populus nigra HCT mutant with modified lignin as a case study

Bartel VanholmeIgor CesarinoGeert GoeminneHoon KimFabio MarroniRebecca Van AckerRuben VanholmeKris MorreelBart IvensSara Pinosio et al.

A1 Journal Article in NEW PHYTOLOGIST

Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis

Ruben VanholmeIgor CesarinoKatarzyna RatajYuguo XiaoLisa SundinGeert GoeminneHoon KimJoanna CrossKris MorreelPedro Araujo et al.

A1 Journal Article in SCIENCE

Lignification in sugarcane: biochemical characterization, gene discovery, and expression analysis in two genotypes contrasting for lignin content

Alexandra BottcherIgor CesarinoAdriana Brombini dos SantosRenato VicentiniJuliana Lischka Sampaio MayerRuben VanholmeKris MorreelGeert GoeminneJullyana Cristina Magalhães Silva MouraPaula Macedo Nobile et al.

A1 Journal Article in PLANT PHYSIOLOGY

Lignin biosynthesis perturbations affect secondary cell wall composition and saccharification yield in Arabidopsis thaliana

Rebecca Van AckerRuben VanholmeVeronique StormeJennifer C MortimerPaul DupreeWout Boerjan

A1 Journal Article in BIOTECHNOLOGY FOR BIOFUELS

Shikimate hydroxycinnamoyl transferase (HCT) activity assays in Populus nigra

Igor CesarinoRuben VanholmeGeert GoeminneBartel VanholmeWout Boerjan

A2 Journal Article in BIO-PROTOCOL

Valorisation potential of natural low HCT poplar mutants in catalytic fast pyrolysis

Jop VercruysseRuben VanholmeBartel VanholmeWout BoerjanWolter PrinsFrederik Ronsse

2012

A systems biology view of responses to lignin biosynthesis perturbations in Arabidopsis

Ruben VanholmeVeronique StormeBartel VanholmeLisa SundinJorgen ChristensenGeert GoeminneClaire HalpinAntje RohdeKris MorreelWout Boerjan

A1 Journal Article in PLANT CELL

Impact of the absence of stem-specific β-glucosidases on lignin and monolignols

Aurélie ChapelleKris MorreelRuben VanholmePhilippe Le-BrisHalima MorinCatherine LapierreWout BoerjanLise JouaninNathalie Demont-Caulet

A1 Journal Article in PLANT PHYSIOLOGY

Metabolic engineering of novel lignin in biomass crops

Ruben VanholmeKris MorreelChiarina DarrahPaula Oyarce SgroJohn H GrabberJohn RalphWout Boerjan

A1 Journal Article in NEW PHYTOLOGIST

2010

Engineering traditional monolignols out of lignin by concomitant up-regulation of F5H1 and down-regulation of COMT in Arabidopsis

Ruben VanholmeJohn RalphTakuya AkiyamaFachuang LuJorge Rencoret PazoHoon KimJorgen ChristensenBrecht Van ReuselVeronique StormeRiet De Rycke et al.

A1 Journal Article in PLANT JOURNAL

Lignin biosynthesis and structure

Ruben VanholmeBrecht DemedtsKris MorreelJohn RalphWout Boerjan

A1 Journal Article in PLANT PHYSIOLOGY

Mass spectrometry-based fragmentation as an identification tool in lignomics

Kris MorreelHoon KimFachuang LuOana DimaTakuya AkiyamaRuben VanholmeClaudiu NiculaesGeert GoeminneDirk InzéEric Messens et al.

A1 Journal Article in ANALYTICAL CHEMISTRY

Mass spectrometry-based sequencing of lignin oligomers

Kris MorreelOana DimaHoon KimFachuang LuClaudiu NiculaesRuben VanholmeRebecca DauweGeert GoeminneDirk InzéEric Messens et al.

A1 Journal Article in PLANT PHYSIOLOGY

Potential of Arabidopsis systems biology to advance the biofuel field

Ruben VanholmeRebecca Van AckerWout Boerjan

A1 Journal Article in TRENDS IN BIOTECHNOLOGY

Walls are thin 1 (WAT1), an Arabidopsis homolog of Medicago truncatula NODULIN21, is a tonoplast-localized protein required for secondary wall formation in fibers

Philippe RanochaNicolas DenanceRuben VanholmeAmandine FreydierYves MartinezLaurent HoffmannLothar KohlerCecile PouzetJean-Pierre RenouBjorn Sundberg et al.

A1 Journal Article in PLANT JOURNAL

2008

Lignin engineering

Ruben VanholmeKris MorreelJohn RalphWout Boerjan

A1 Journal Article in CURRENT OPINION IN PLANT BIOLOGY

2004

High-speed calculation of AIM charges through the electronegativity equalization method

Patrick BultinckRuben VanholmePLA POPELIERF DE PROFTP GEERLINGS

A1 Journal Article in JOURNAL OF PHYSICAL CHEMISTRY A