Research Explorer

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Researcher

Roland Contreras

Awards & Distinctions

152 Results

2020

Off-target glycans encountered along the synthetic biology route toward humanized N-glycans in Pichia pastoris

Bram LaukensPieter JacobsKatelijne GheysenJosé MartinsCharlot De WachterPaul AmelootWilly MorelleJurgen HaustraeteJean-Christophe RenauldBart Samyn et al.

A1 Journal Article in BIOTECHNOLOGY AND BIOENGINEERING

2010

Alteration of N-glycome in diethylnitrosamine-induced hepatocellular carcinoma mice: a non-invasive monitoring tool for liver cancer

Xue-en LiuSylviane DewaeleValerie VanhoorenYedong FanLing WangJacques Van HuysseHui ZhuangRoland ContrerasClaude LibertCuiying Chen

A1 Journal Article in LIVER INTERNATIONAL

Altered serum N-glycomics in chronic hepatitis B patients

Hong-Lian GuiChung-Fang GaoHui WangXue-En LiuQing XieSylviane DewaeleLing WangHui ZhuangRoland ContrerasClaude Libert et al.

A1 Journal Article in LIVER INTERNATIONAL

Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate

Pieter JacobsM InanNele FestjensJurgen HaustraeteAnnelies Van HeckeRoland ContrerasMM MeagherNico Callewaert

A1 Journal Article in MICROBIAL CELL FACTORIES

2009

Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology

Pieter P. JacobsSteven GeysensWouter VerveckenRoland ContrerasNico Callewaert

A1 Journal Article in NATURE PROTOCOLS

N-glycan profiles as tools in diagnosis of hepatocellular carcinoma and prediction of healthy human ageing

Valerie VanhoorenXue-En LiuClaudio FranceschiChun-Fang GaoClaude LibertRoland ContrerasCuiying Chen

A1 Journal Article in MECHANISMS OF AGEING AND DEVELOPMENT

Reversible changes in serum immunoglobulin galactosylation during the immune response and treatment of inflammatory autoimmune arthritis

Katrien Van BenedenKen CoppietersW LaroyFilip De KeyserIlse HoffmanFilip Van den BoschBert Vander CruyssenMichael DrennanPeggy JacquesP Rottiers et al.

A1 Journal Article in ANNALS OF THE RHEUMATIC DISEASES

Serum protein N-glycans profiling for the discovery of potential biomarkers for nonalcoholic steatohepatitis

Cuiying ChenHemda Schmilovtz-WeissXue-en LiuOrit PappoMarisa HalpernJacqueline SulkesMarius BraunMaya CohenNir BarakRan Tur-Kaspa et al.

A1 Journal Article in JOURNAL OF PROTEOME RESEARCH

2008

Contribution of galactofuranose to the virulence of the opportunistic pathogen Aspergillus fumigatus

PS SCHMALHORSTS KRAPPMANNWouter VerveckenM ROHDEM MULLERGH BRAUSRoland ContrerasA BRAUNH BAKKERFH ROUTIER

A1 Journal Article in EUKARYOTIC CELL

Fishing for lectins from diverse sequence libraries by yeast surface display - An exploratory study

Stefan RyckaertNico CallewaertPieter JacobsSylviane DewaeleIsabelle DewerteRoland Contreras

A1 Journal Article in GLYCOBIOLOGY

Glycosylation and cell surface engineering in Pichia pastoris

Pieter P JacobsNico CallewaertRoland Contreras

Immunization with an engineered mutant trans-sialidase highly protects mice from experimental Tryponosoma cruzi infection: A vaccine candidate

GH FONTANELLAKristof De VusserWouter LaroyL DAURELIOAL NOCITOS REVELLIRoland Contreras

A1 Journal Article in VACCINE

Over-expression of heat shock protein 70 in mice is associated with growth retardation, tumor formation, and early death

Valerie VanhoorenXue-En LiuLiesbeth DesmyterYe-Dong FanLieve VanwalleghemWim Van MolleSylviane DewaeleMarleen PraetRoland ContrerasClaude Libert et al.

A1 Journal Article in REJUVENATION RESEARCH

Pichia surface display: display of proteins on the surface of glycoengineered Pichia pastoris strains

Pieter JacobsStefan RyckaertSteven GeysensKristof De VusserNico CallewaertRoland Contreras

A1 Journal Article in BIOTECHNOLOGY LETTERS

2007

Clearance mechanism of a mannosylated antibody-enzyme fusion protein used in experimental cancer therapy

H KOGELBERGB TOLNERSK SHARMAMW LOWDELLU QURESHIM ROBSONT HILLYERRB PEDLEYWouter VerveckenRoland Contreras et al.

A1 Journal Article in GLYCOBIOLOGY

Dependence of neurotrophic factor activation of Trk tyrosine kinase receptors on cellular sialidase

A WORONOWICZSR AMITHKristof De VusserWouter LaroyRoland ContrerasS BASTAMR SZEWCZUK

A1 Journal Article in GLYCOBIOLOGY

From laboratory to Phase I/II cancer trials with recombinant biotherapeutics

B TOLNERL SMITHT HILLYERJ BHATIAP BECKETTL ROBSONSK SHARMAN GRIFFINWouter VerveckenRoland Contreras et al.

A1 Journal Article in EUROPEAN JOURNAL OF CANCER

Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88

HJ PELJH DE WINDEDB ARCHERPS DYERG HOFMANNPJ SCHAAPG TURNERRP DE VRIESR ALBANGK ALBERMANN et al.

A1 Journal Article in NATURE BIOTECHNOLOGY

Glycoprofiling by DNA sequence-aided fluorophore-assisted carbohydrate electrophoresis: new opportunities in diagnosing and following disease

Wouter LaroyRoland Contreras

Bookchapter in BioArrays: From Basics to Diagnostics

Identifying genes that extend life-span using a high throughput screening system

Cuiying ChenRoland Contreras

Bookchapter in Biological Aging: Methods and Protocols

Modification of the N-glycosylation pathway to produce homogeneous human-like glycans using GlycoSwitch plasmids

Wouter VerveckenNico CallewaertVladimir KaigorodovSteven GeysensRoland Contreras

Bookchapter in Pichia Protocols

N-glycomic changes in hepatocellular carcinoma patients with liver cirrhosis induced by hepatitis B virus

Xueen LiuLiesbeth DesmyterCF GAOWouter LaroySylviane DewaeleValerie VanhoorenL WANGH ZHUANGNico CallewaertClaude Libert et al.

A1 Journal Article in HEPATOLOGY

N-glycomic changes in serum proteins during human aging

Valerie VanhoorenLiesbeth DesmyterXueen LiuM CARDELLIC FRANCESCHIA FEDERICOClaude LibertWouter LaroySylviane DewaeleRoland Contreras et al.

A1 Journal Article in REJUVENATION RESEARCH

Nonclassical export pathway: overexpression of NCE102 reduces protein and DNA damage and prolongs lifespan in an SGS1 deficient Saccharomyces cerevisiae

Liesbeth DesmyterJan VerstraelenSylviane DewaeleClaude LibertRoland ContrerasCuiying Chen

A1 Journal Article in BIOGERONTOLOGY

Rating of CCl4-induced rat liver fibrosis by blood serum glycomics

Liesbeth DesmyterYedong FanMarleen PraetT JAWORSKIWouter VerveckenBernard de HemptinneRoland ContrerasCuiying Chen

A1 Journal Article in JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY

Trypanosome trans-sialidase mediates neuroprotection against oxidative stress, serum/glucose deprivation, and hypoxia-induced neurite retraction in Trk-expressing PC12 cells

A WORONOWICZSR AMITHVW DAVISP JAYANTHKristof De VusserWouter LaroyRoland ContrerasSO MEAKINMR SZEWCZUK

A1 Journal Article in GLYCOBIOLOGY

2006

Glycome mapping on DNA sequencing equipment

Wouter LaroyRoland ContrerasNico Callewaert

A1 Journal Article in NATURE PROTOCOLS

Secretion of functional human enzymes by Tetrahymena thermophila

T WEIDEL HERRMANNU BOCKAUN NIEBURI ALDAGWouter LaroyRoland ContrerasA TIEDTKEMWW HARTMANN

A1 Journal Article in BMC BIOTECHNOLOGY

The innovative serum protein N-glycans analysis predicts the extent of hepatic fibrosis in patients with nonalcoholic fatty liver disease

H SCHMILOVITZ-WEISSCuiying ChenRoland ContrerasO PAPPOM HALPERNJ SULKESM BRAUNM COHENN BARAKR TUR-KASPA et al.

2005

Cloning and characterization of the glucosidase II alpha subunit gene of Trichoderma reesei: a frameshift mutation results in the aberrant glycosylation profile of the hypercellulolytic strain Rut-C30

Steven GeysensT PAKULAJ UUSITALOIsabelle DewerteM PENTTILARoland Contreras

A1 Journal Article in APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Development of a S-cerevisiae whole cell biocatalyst for in vitro sialylation of oligosaccharides

Stefan RyckaertVera MartensKristof De VusserRoland Contreras

A1 Journal Article in JOURNAL OF BIOTECHNOLOGY

Glycoprofiling of N-linked serum protein: an aging biomarker?

Cuiying ChenL DESMYTERW VAN MOLLEWouter LaroyAnnelies Van HeckeSylviane DewaeleA FEDERICOClaude LibertRoland Contreras

Human antimicrobial peptides: defensins, cathelicidins and histatins

Kris De SmetRoland Contreras

A1 Journal Article in BIOTECHNOLOGY LETTERS

Independent validation and comparison with fibroscan, fibrotest and liver biopsy of clinical glycomics for the non invasive assessment of liver fibrosis in chronic hepatitis C

L CASTERAAnnelies Van HeckeP TRIMOULETNico CallewaertP COUZIGOUV DE LEDINGHENRoland ContrerasWouter Laroy

N-glycan engineering in yeasts and fungi: finally human-like glycosylation

Nico CallewaertWouter VerveckenSteven GeysensRoland Contreras

Bookchapter in Handbook of Carbohydrate Engineering

Old yellow enzyme interferes with Bax-induced NADPH loss and lipid peroxidation in yeast

Rieka ReekmansKris De SmetCuiying ChenP VAN HUMMELENRoland Contreras

A1 Journal Article in FEMS YEAST RESEARCH

2004

Bax-induced cell death in Candida albicans

Kris De SmetInes EberhardtRieka ReekmansRoland Contreras

A1 Journal Article in YEAST

DSA-FACE: high-throughput analysis of the N-glycans of NS0-cell secreted antibodies

Lieselot DefrancqNico CallewaertJ ZHUWouter LaroyRoland Contreras

A2 Journal Article in Bioprocess International

Expression of the human ferritin light chain in a frataxin mutant yeast affects ageing and cell death

Liesbeth DesmyterSylviane DewaeleRieka ReekmansT NYSTROMRoland ContrerasCuiying Chen

A1 Journal Article in EXPERIMENTAL GERONTOLOGY

Factors influencing glycosylation of Trichoderma reesei cellulases. I: Postsecretorial changes of the O- and N-glycosylation pattern of Cel7A

Ingeborg StalsKoen SandraSteven GeysensRoland ContrerasJozef Van BeeumenMarc Claeyssens

A1 Journal Article in GLYCOBIOLOGY

HSAbodies: A new class of engineered antibody-based molecules for targeting cancer

A HUHALOVC GRAFFKD WITTRUPB TOLNERRB PEDLEYWouter VerveckenRoland ContrerasRHJ BEGENTKA CHESTER

In vivo synthesis of mammalian-like, hybrid-type N-glycans in Pichia pastoris

Wouter VerveckenVladimir KaigorodovNico CallewaertSteven GeysensKristof De VusserRoland Contreras

A1 Journal Article in APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Noninvasive diagnosis of liver cirrhosis using DNA sequencer-based total serum protein glycomics

Nico CallewaertHans Van VlierbergheAnnelies Van HeckeWouter LaroyJoris DelangheRoland Contreras

A1 Journal Article in NATURE MEDICINE

Role of oxidative phosphorylation in histatin 5-induced cell death in Saccharomyces cerevisiae

Kris De SmetRieka ReekmansRoland Contreras

A1 Journal Article in BIOTECHNOLOGY LETTERS

The bud scar-based screening system for hunting human genes extending life span

Cuiying ChenRoland Contreras

A1 Journal Article in STRATEGIES FOR ENGINEERED NEGLIBLE SENESCENCE: WHY GENUINE CONTROL OF AGING MAY BE FORESEEABLE

Total serum protein N-glycome profiling on a capillary electrophoresis-microfluidics platform

Nico CallewaertRoland ContrerasL MITNIK-GANKINL CAREYP MATSUDAIRAD EHRLICH

A1 Journal Article in ELECTROPHORESIS

Trypanosome trans-sialidase targets TrkA tyrosine kinase receptor and induces receptor internalization and activation

A WORONOWICZKristof De VusserWouter LaroyRoland ContrerasSO MEAKINGM ROSSMR SZEWCZUK

A1 Journal Article in GLYCOBIOLOGY

2003

A high-throughput screening system for genes extending life-span

Cuiying ChenSylviane DewaeleBart BraeckmanLiesbeth DesmyterJan VerstraelenGaetan BorgonieJacques VanfleterenRoland Contreras

A1 Journal Article in EXPERIMENTAL GERONTOLOGY

Increased fucosylation and reduced branching of serum glycoprotein N-glycans in all known subtypes of congenital disorder of glycosylation I

Nico CallewaertE SCHOLLENA VANHECKEJ JAEKENG MATTHIJSRoland Contreras

A1 Journal Article in GLYCOBIOLOGY

2002

Non-pathogenic trypanosomatid protozoa as a platform for protein research and production.

R BREITLINGS KLINGNERNico CallewaertR PIETRUCHAA GEYERG EHRLICHR HARTUNGA MULLERRoland ContrerasSM BEVERLEY et al.

A1 Journal Article in PROTEIN EXPRESSION AND PURIFICATION

Novel tools for the study of class I alpha-mannosidases: a chromogenic substrate and a substrate-analog inhibitor.

Tom DesmetWim NerinckxIngeborg StalsNico CallewaertRoland ContrerasMarc Claeyssens

A1 Journal Article in ANALYTICAL BIOCHEMISTRY

Structure and function in rhodopsin: High-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line.

PJ REEVESNico CallewaertRoland ContrerasHG KHORANA

A1 Journal Article in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Use of a meltable polyacrylamide matrix for sodium dodecyl sulfate-polyacrylamide gel electrophoresis in a procedure for N-glycan analysis on picomole amounts of glycoproteins.

Nico CallewaertWouter VerveckenA VAN HECKERoland Contreras

A1 Journal Article in ANALYTICAL BIOCHEMISTRY

2001

An antisense-based functional genomics approach for identification of genes critical for growth of Candida albicans.

MD DE BACKERB NELISSENMarc LoggheJasmine ViaeneI LOONENS VANDONINCKR DE HOOGTS DEWAELEFA SIMONSP VERHASSELT et al.

A1 Journal Article in NATURE BIOTECHNOLOGY

Characterization of sialyltransferase mutants using surface plasmon resonance.

Wouter LaroyPaul AmelootRoland Contreras

A1 Journal Article in GLYCOBIOLOGY

Trichoderma reesei alpha-1,2-mannosidase: Structural basis for the cleavage of four consecutive mannose residues.

Filip Van PetegemH CONTRERASRoland ContrerasJozef Van Beeumen

A1 Journal Article in JOURNAL OF MOLECULAR BIOLOGY

Ultrasensitive profiling and sequencing of N-linked oligosaccharides using standard DNA-sequencing equipment.

Nico CallewaertSteven GeysensP MOLEMANSRoland Contreras

A1 Journal Article in GLYCOBIOLOGY

Use of HDEL-tagged Trichoderma reesei mannosyl oligosaccharide 1,2-alpha-D-mannosidase for N-glycan engineering in Pichia pastoris.

Nico CallewaertWouter LaroyH CADIRGISteven GeysensXavier SaelensWilly Min JouRoland Contreras

A1 Journal Article in FEBS LETTERS

2000

Cloning and expression of cytokine genes

Wim DeclercqMarc LoggheWalther FiersRoland Contreras

Bookchapter in Cytokine molecular biology : a practical approach

Cloning of Trypanosoma cruzi trans-sialidase and Expression in Pichia pastoris.

Wouter LaroyRoland Contreras

A1 Journal Article in PROTEIN EXPRESSION AND PURIFICATION

MET15 as a visual selection marker for Candida albicans.

Jasmine ViaeneP TIELSMarc LoggheS DEWAELEWim MartinetRoland Contreras

A1 Journal Article in YEAST

Molecular cloning and enzymatic characterization of a Trichoderma reesei 1,2-alpha-D-mannosidase.

Marleen MarasNico CallewaertKathleen PiensMarc ClaeyssensWim MartinetS DEWAELEH CONTRERASI DEWERTEM PENTTILARoland Contreras

A1 Journal Article in JOURNAL OF BIOTECHNOLOGY

PCR-and ligation-mediated synthesis of split-marker cassettes with long flanking homology regions for gene disruption in Candida albicans.

R DE HOOGTWHML LUYTENRoland ContrerasMD DE BACKER

A1 Journal Article in BIOTECHNIQUES

Single allele knock-out of Candida albicans CGT1 leads to unexpected resistance to hygromycin B and elevated temperature.

MD DE BACKERRA DE HOOGTG FROYENFC ODDSF SIMONSRoland ContrerasW LUYTEN

A1 Journal Article in MICROBIOLOGY-UK

1999

Bax-induced cell death in Pichia pastoris.

Wim MartinetD VAN DEN PLASHilda RaesRieka ReekmansRoland Contreras

A1 Journal Article in BIOTECHNOLOGY LETTERS

Filamentous fungi as production organisms for glycoproteins of bio-medical interest.

Marleen MarasI VAN DIERoland ContrerasCAMJJ VAN DEN HONDEL

A1 Journal Article in GLYCOCONJUGATE JOURNAL

Identificatie en karakterisering van cellulaire inhibitoren van NF-kB-afhankelijke genexpressie

Karen HeyninckRoland ContrerasRudi Beyaert

In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei

Marleen MarasAndré De BruynWouter VerveckenJ UusitaloM PenttiläR BussonPiet HerdewijnRoland Contreras

A1 Journal Article in FEBS LETTERS

Protection of mice against a lethal influenza virus challenge after immunization with yeast-derived secreted influenza virus hemagglutinin.

Xavier SaelensP VANLANDSCHOOTWim MartinetMarleen MarasSabine NeirynckRoland ContrerasWalther FiersWilly Min Jou

A1 Journal Article in EUROPEAN JOURNAL OF BIOCHEMISTRY

Structure-function analysis of human liver a 2, 6-sialyltransferase.

Wouter LaroyMarleen MarasRoland Contreras

Bookchapter in Sialobiology and Other Novel Forms of Glycosylation / Edited by Y. Inoue, Y.C. Lee and F.A. Troy II. - Osaka : Gakushin, 1999. - 308 p. - ISBN 4-907773-00-5

The zinc finger protein A20 inhibits TNF-induced NF-kappa B-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal and directly binds to a novel NF-kappa B-inhibiting protein ABIN.

Karen HeyninckDirk De ValckWim Vanden BergheWim Van CriekingeRoland ContrerasWalther FiersGuy HaegemanRudi Beyaert

A1 Journal Article in JOURNAL OF CELL BIOLOGY

The zinc finger protein A20 interacts with a novel anti-apoptotic protein which is cleaved by specific caspases.

Dirk De ValckDY JINKaren HeyninckMarc Van De CraenRoland ContrerasWalther FiersKT JEANGRudi Beyaert

A1 Journal Article in ONCOGENE

Transformation of Candida albicans by electroporation.

MD DE BACKERD MAESS VANDONINCKMarc LoggheRoland ContrerasW LUYTEN

A1 Journal Article in YEAST

Tumoral environment triggers transcript anomalies in established tumors : induction of altered gene expression and of aberrant, truncated and B2 repeat-containing gene transcripts

Pieter RottiersMarjory DesmedtHans DoomsRoland ContrerasJohan Grooten

A2 Journal Article in NEOPLASIA

1998

Differentiation of EL4 lymphoma cells by tumoral environment is associated with inappropriate expression of the large chondroitin sulfate proteoglycan PG-M and the tumor-associated antigen HTgp-175.

Pieter RottiersT VERFAILLIERoland ContrerasH REVETSMarjory DesmedtH DOOMSWalther FiersJohan Grooten

A1 Journal Article in INTERNATIONAL JOURNAL OF CANCER

Engineering of the carbohydrate moiety of fungal proteins to a mammalian type

Marleen MarasA De BruynJ SchramlP HerdewijnKathleen PiensMarc ClaeyssensJ UusitaloM PenttilaWalther FiersRoland Contreras

Bookchapter in Carbohydrates from Trichoderma reesei and other microorganisms : structures, biochemistry, genetics and applications

Evaluation of recombinant A/Victoria/3/75 (H3N2) influenza neuraminidase mutants as potential broad-spectrum subunit vaccines against influenza A.

Wim MartinetTom DerooXavier SaelensEls BeirnaertPeter VanlandschootRoland ContrerasWalther FiersWilly Min Jou

A1 Journal Article in ARCHIVES OF VIROLOGY

Identificatie en karakterisering van fosfolipasen en TNF-resistente eiwitten betrokken bij TNF-geïnduceerde celdood en genactivering

Dirk De ValckRudi BeyaertRoland Contreras

Karakterisering van caspasen in een '2 en 3-hybrid'-systeem

Wim Van CriekingeRudi BeyaertWalther FiersRoland Contreras

Modification of the protein glycosylation pathway in the methylotrophic yeast Pichia pastoris.

Wim MartinetMarleen MarasXavier SaelensWM JOURoland Contreras

A1 Journal Article in BIOTECHNOLOGY LETTERS

NMR techniques in the study of protein-linked carbohydrates

André De BruynJ SchramlMarleen MarasRoland ContrerasP Herdewijn

Protein secretion and glycosylation in Trichoderma

G PALAMARCZYKMarleen MarasRoland ContrerasJ KRUSZEWSKA

1997

A radioactive assay for sialyltransferase activity using 96-well multiscreen filtration plates.

Wouter LaroyMarleen MarasWalther FiersRoland Contreras

A1 Journal Article in ANALYTICAL BIOCHEMISTRY

A versatile NMR technique for the identification of phosphorylation sites in oligosaccharides

J SchramlAndré De BruynRoland ContrerasPiet Herdewijn

A1 Journal Article in JOURNAL OF CARBOHYDRATE CHEMISTRY

Hadamard 1D 1H TOCSY and its application to oligosaccharides

J SchramlH van HalbeekAndré De BruynRoland ContrerasMarleen MarasPiet Herdewijn

A1 Journal Article in MAGNETIC RESONANCE IN CHEMISTRY

In vitro conversion of the carbohydrate moiety of fungal glycoproteins to mammalian-type oligosaccharides - Evidence for N-acetylglucosaminyltransferase-I-accepting glycans from Trichoderma reesei.

Marleen MarasXavier SaelensWouter LaroyK PIENSMarc ClaeyssensWalther FiersRoland Contreras

A1 Journal Article in EUROPEAN JOURNAL OF BIOCHEMISTRY

In vitro conversion of the conversion of the carbohydrate moiety of fungal glycoproteins to mammalian-type oligosaccharides. Evidence for iV-acetylglucosaminyltransferase-I-accepting glycans from Trichoderma reesei

M MARASX SAELENSWouter LaroyKathleen PiensMarc ClaeyssensWalther FiersRoland Contreras

A2 Journal Article in Eur. J. Biochem., 2 45, 617-625

Isolation and analysis of functional homologues of the secretion-related SAR1 gene of Saccharomyces cerevisiae from Aspergillus niger and Trichoderma reesei.

G VELDHUISENM SALOHEIMOMA FIERSPJ PUNTRoland ContrerasM PENTTILACAMJJ VANDENHONDEL

A1 Journal Article in MOLECULAR AND GENERAL GENETICS

NMR evidence for a novel asparagine-linked oligosaccharide on cellobiohydrolase I from Trichoderma reesei RUTC 30

André De BruynMarleen MarasJ. SchramlPiet HerdewijnRoland Contreras

A1 Journal Article in FEBS LETTERS

Protection of mice against a lethal influenza challenge by immunization with yeast-derived recombinant influenza neuraminidase.

Wim MartinetXavier SaelensTom DerooSabine NeirynckRoland ContrerasWilly Min JouWalther Fiers

A1 Journal Article in EUROPEAN JOURNAL OF BIOCHEMISTRY

Structural characterization of N-linked oligosaccharides from cellobiohydrolase I secreted by the filamentous fungus Trichoderma reesei RUTC30

Marleen MarasAndré De BruynJ SchramlPiet HerdewijnMarc ClaeyssensWalther FiersRoland Contreras

A1 Journal Article in EUROPEAN JOURNAL OF BIOCHEMISTRY

1996

A20, an inhibitor of cell death, self-associates by its zinc finger domain.

Dirk De ValckKaren HeyninckWim Van CriekingeRoland ContrerasRudi BeyaertWalther Fiers

A1 Journal Article in FEBS LETTERS

1995

Cloning and expression of cytokine genes

Roland ContrerasJan DemolderWalther Fiers

Bookchapter in Cytokines : a practical approach

ENGINEERING OF THE CARBOHYDRATE MOIETY OF FUNGAL GLYCOPROTEINS TO A MAMMALIAN TYPE.

M MARASWalther FiersRoland Contreras

A1 Journal Article in PROTEIN ENGINEERING

PROTEIN-PRODUCTION BY THE FILAMENTOUS FUNGUS TRICHODERMA-REESEI - SECRETION OF ACTIVE ANTIBODY MOLECULES.

E NyyssonenJan DemolderRoland ContrerasS KeranenM Penttila

A1 Journal Article in CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE

1994

COMPLETE DNA-SEQUENCE OF YEAST CHROMOSOME-II.

H FELDMANNM AIGLEG ALJINOVICB ANDREMC BACLETC BARTHEA BAURAM BECAMN BITEAUE BOLES et al.

A1 Journal Article in EMBO JOURNAL

Human interferon-beta, expressed in Saccharomyces cerevisiae, is predominantly directed to the vacuoles. Influence of modified co-expression of secretion factors and chaperones

Jan DemolderWalther FiersRoland Contreras

A1 Journal Article in JOURNAL OF BIOTECHNOLOGY

PHOSPHORIBOSYLPYROPHOSPHATE SYNTHETASE (PRS) - A NEW GENE FAMILY IN SACCHAROMYCES-CEREVISIAE.

AT CARTERA NARBADBM PEARSONKF BECKB BAUMM LOGGHERoland ContrerasM SCHWEIZER

A1 Journal Article in YEAST

SEQUENCE-ANALYSIS AND EXPRESSION STUDIES OF A GENE ENCODING A NOVEL SERINE PLUS ALANINE-RICH PROTEIN IN TRICHODERMA-HARZIANUM.

GH GOLDMANV VASSEURRoland ContrerasMarc Van Montagu

A1 Journal Article in GENE

THE 2 GENES ENCODING YEAST RIBOSOMAL-PROTEIN S8 RESIDE ON DIFFERENT CHROMOSOMES, AND ARE CLOSELY LINKED TO THE HSP70 STRESS PROTEIN GENE-SSA3 AND GENE-SSA4.

M LOGGHEFrancis MolemansWalther FiersRoland Contreras

A1 Journal Article in YEAST

The two genes encoding yeast ribosomal protein S8 reside on different chromosomes, and are closely linked to the hsp7O stress protein genes SSA3 and SSA4.

Marc LoggheFrancis MolemansWalther FiersRoland Contreras

A2 Journal Article in YEAST

1993

A nucleotide substitution in one of the beta-tubulin genes of Trichoderma viride confers resistance to the antimitotic drug methyl benzimidazole-2-yl-carbamate

GH GoldmanWim TemmermanD JacobsRoland ContrerasMarc Van MontaguA Herrera Estrella

A1 Journal Article in MOLECULAR AND GENERAL GENETICS

Phenotypic effects in Saccharomyces cerevisiae after regulated expression of beta-(l,3)-glucanase from Nicotiana plumbaginifolia

Jan DemolderM De BackerWalther FiersRoland Contreras

A1 Journal Article in JOURNAL OF BIOTECHNOLOGY

SECRETION OF HETEROLOGOUS PROTEINS BY ASPERGILLUS-NIGER - PRODUCTION OF ACTIVE HUMAN INTERLEUKIN-6 IN A PROTEASE-DEFICIENT MUTANT BY KEX2-LIKE PROCESSING OF A GLUCOAMYLASE-HIL6 FUSION PROTEIN.

MP BROEKHUIJSENIE MATTERNRoland ContrerasJR KINGHORNCAMJJ VANDENHONDEL

A1 Journal Article in JOURNAL OF BIOTECHNOLOGY

1992

Characterization of the carbohydrate moiety of glycoproteins from different yeast species

Marleen MarasJan DemolderW HersRoland Contreras

Efficient synthesis of secreted murine interleukin-2 by Saccharomyces cerevisiae: Influence of 3'-untranslated regions and codon usage.

Jan DemolderWalther FiersRoland Contreras

A1 Journal Article in GENE

Molecular cloning of the imidazoleglycerolphosphate dehydratase gene of Trichoderma harzianum by genetic complementation in Saccharomyces cerevisiae using a direct expression vector

GH GoldmanJan DemolderS DewaeleA Herrara EstrellaRoberto GeremiaMarc Van MontaguRoland Contreras

A1 Journal Article in MOLECULAR AND GENERAL GENETICS

THE ASPERGILLUS-NIGER-NIAD GENE ENCODING NITRATE REDUCTASE - UPSTREAM NUCLEOTIDE AND AMINO-ACID-SEQUENCE COMPARISONS.

SE UNKLESEI CAMPBELLPJ PUNTKL HAWKERRoland ContrerasAR HAWKINSCAMJJ VANDENHONDELJR KINGHORN

A1 Journal Article in GENE

1991

Efficient KEX2-like processing of a glucoamylase-interleukin-6 fusion protein by Aspergillus nidulans and secretion of mature interleukin-6

Roland ContrerasDirk CarrezJames R KinghornCees AMJJ van den HondelWalther Fiers

A1 Journal Article in BIO-TECHNOLOGY

Production and purification of recombinant human interleukin-6 secreted by the yeast Saccharomyces cerevisiae

Yves GuisezBernard TisonJoël VandekerckhoveJan DemolderGuy BauwGuy HaegemanWalther FiersRoland Contreras

A1 Journal Article in EUROPEAN JOURNAL OF BIOCHEMISTRY

The nucleotide sequence of a third cyclophilin-homologous gene from Saccharomyces cerevisiae

L FrancoA JiménezJan DemolderFrancis MolemansWalther FiersRoland Contreras

A1 Journal Article in YEAST

1990

Heterologous gene expression by filamentous fungi: secretion of human interleukin-6 by Aspergillus nidulans

Dirk CarrezWouter JanssensPatrick DegraveCees AMJJ van den HondelJames R KinghornWalther FiersRoland Contreras

A1 Journal Article in GENE

1989

A gene transfer system based on the homologous pyrG gene and efficient expression of bacterial genes in Aspergillus oryzae

Yolanda MJT de Ruiter-JacobsMartien BroekhuijsenShiela E UnklesEdward I CampbellJanes R KinghornRoland ContrerasPeter H PouwelsCees AMJJ van den Hondel

A1 Journal Article in CURRENT GENETICS

Expression of human and murine interleukin-5 in eukaryotic systems

Jan TavernierRené DevosJosé Van der HeydenGuido HauquierRita BaudenIna FachéEric KawashimaJoël VandekerckhoveRoland ContrerasWalther Fiers

A1 Journal Article in DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY

Improved transformation efficiency of Aspergillus niger using the homologous niaD gene for nitrate reductase

Edward I CampbellShiela E UnklesJanet A MacroCees van den HondelRoland ContrerasJames R Kinghorn

A1 Journal Article in CURRENT GENETICS

Transformation of Aspergillus niger with the homologous nitrate reductase gene

SE UnklesEI CampbellDirk CarrezC GrieveRoland ContrerasWalther FiersCAMJJ Van den HondelJR Kinghorn

A1 Journal Article in GENE

Transformation of a nitrate reductase deficient mutant of Penicillium chrysogenum with the corresponding Aspergillus niger and A. nidulans niaD genes

Michael P WhiteheadShiela E UnklesMartin RamsdenEdward I CampbellSarah Jane GurrDavid SpenceCess Van den HondelRoland ContrerasJames R Kinghorn

A1 Journal Article in MOLECULAR & GENERAL GENETICS

1987

The 3' untranslated region of the human interferon-β mRNA has an inhibitory effect on translation

V KruysM WatheletP PoupartRoland ContrerasWalther FiersJ ContentG Huez

A1 Journal Article in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

1986

Construction of stable laboratory and industrial yeast strains expressing a foreign gene by integrative transformation using a dominant selection system

JingDong ZhuRoland ContrerasWalther Fiers

A1 Journal Article in GENE

1985

A method for fast and pure DNA elution from agarose gels by centrifugal filtration

JingDong ZhuWalter KempenaersDominique Van Der StraetenRoland ContrerasWalther Fiers

A1 Journal Article in BIO-TECHNOLOGY

1983

Simian virus 40 mutants carrying extensive deletions in the 72-base-pair repeat region

Dirk GheysenAndré Van De VoordeRoland ContrerasJosé Van der HeydenFred DuerinckWalther Fiers

A1 Journal Article in JOURNAL OF VIROLOGY

1982

Evidence for the direct involvement of DNA replication origin in synthesis of late SV40 RNA

Roland ContrerasDirk GheysenJ KnowlandAndré Van de VoordeWalther Fiers

A1 Journal Article in NATURE

Simple, efficient in vitro synthesis of capped RNA useful for direct expression of cloned eukaryotic genes

Roland ContrerasHilde CheroutreWim DegraveWalther Fiers

A1 Journal Article in NUCLEIC ACIDS RESEARCH

The human fibroblast and human immune interferon genes and their expression in homologous and heterologous cells

Walther FiersErik RemautRené DevosHilde CheroutreRoland ContrerasDirk GheysenWim DegravePatrick StanssensJan TavernierYoichi Taya et al.

A1 Journal Article in PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES

1981

A simple apparatus for injection of nanoliter quantities into Xenopus laevis oocytes

Roland ContrerasHilde CheroutreWalther Fiers

A1 Journal Article in ANALYTICAL BIOCHEMISTRY

Initiation of transcription by RNA polymerase II in permeable, SV40-infected or noninfected, CV1 cells : evidence for multiple promoters of SV40 late transcription

Roland ContrerasWalther Fiers

A1 Journal Article in NUCLEIC ACIDS RESEARCH

1979

Asymmetric linker molecules for recombinant DNA constructions

Kjeld E NorrisDirk IserentantRoland ContrerasWalther Fiers

A1 Journal Article in GENE

Construction and characterization of a plasmid containing a nearly full-size DNA copy of bacteriophage MS2 RNA

René DevosJohn Van EmmeloRoland ContrerasWalther Fiers

A1 Journal Article in JOURNAL OF MOLECULAR BIOLOGY

Identification of the translocatable element IS1 in a molecular chimera constructed with plasmid pBR322 DNA into which a bacteriophage MS2 DNA copy was inserted by the poly(dA)·Poly(dT) linker method

René DevosRoland ContrerasJohn Van EmmeloWalther Fiers

A1 Journal Article in JOURNAL OF MOLECULAR BIOLOGY

Nucleotide sequence analysis of two simian virus 40 mutants with deletions in the late region of the genome

Roland ContrerasCharles ColePaul BergWalther Fiers

A1 Journal Article in JOURNAL OF VIROLOGY

Total synthesis of a tyrosine suppressor transfer RNA gene, XVI: enzymatic joinings to form the total 207-base pair-long DNA

Takao SekiyaTatsuo TakeyaEugene L BrownRamamoorthy BelagajaRoland ContrerasHans-Joachim FritzMichael J GaitRobert G LeesMichael J RyanH Gobind Khorana et al.

A1 Journal Article in JOURNAL OF BIOLOGICAL CHEMISTRY

Total synthesis of a tyrosine suppressor transfer RNA gene, XVII: transcription, in vitro, of the synthetic gene and processing of the primary transcript to transfer RNA

Takao SekiyaRoland ContrerasTatsuo TakeyaH Gobind Khorana

A1 Journal Article in JOURNAL OF BIOLOGICAL CHEMISTRY

1978

Complete nucleotide sequence of SV40 DNA

Walther FiersRoland ContrerasGuy HaegemanRaphaël RogiersAndré Van De VoordeHugo Van HeuverswynJacqueline Van HerrewegheGuido VolckaertMarc Ysebaert

A1 Journal Article in NATURE

Nucleotide sequence of the restriction fragment Hind-F-EcoRI1 of Simian-Virus-40 DNA (part of the VP1 gene)

Roland ContrerasAndré Van De VoordeWalther Fiers

A1 Journal Article in EUROPEAN JOURNAL OF BIOCHEMISTRY

1977

Nucleotide sequence of simian virus 40 Hind H restriction fragment

Guido VolckaertRoland ContrerasE SoedaAndré Van de VoordeWalther Fiers

A1 Journal Article in JOURNAL OF MOLECULAR BIOLOGY

Nucleotide sequence of the restriction fragment Hind F-Eco RI2 of SV40 DNA

Roland ContrerasGuido VolckaertF ThysAndré Van de VoordeWalther Fiers

A1 Journal Article in NUCLEIC ACIDS RESEARCH

Overlapping of the VP2-VP3 gene and the VP1 gene in the SV40 genome

Roland ContrerasRaphaël RogiersAndré Van de VoordeWalther Fiers

A1 Journal Article in CELL

1976

Complete nucleotide sequence of bacteriophage MS2 RNA: primary and secondary structure of the replicase gene

Walther FiersRoland ContrerasFred DuerinckGuy HaegemanDirk IserentantJozef MerregaertWilly Min JouFrancis MolemansAlex RaeymaekersA Van den Berghe et al.

A1 Journal Article in NATURE

Escherichia coli tyrosine transfer ribonucleic acid genes: nucleotide sequences of their promoters and of the regions adjoining C-C-A ends

Takao SekiyaRoland ContrerasHans KüpperArthur LandyH Gobind Khorana

A1 Journal Article in JOURNAL OF BIOLOGICAL CHEMISTRY

The initiation region of the SV40 VP1 gene

André Van de VoordeRoland ContrerasRaphaël RogiersWalther Fiers

A1 Journal Article in CELL

1975

A method for the isolation of cytidylate series from ribonuclease T1-oligonucleotides

Roland ContrerasWalther Fiers

A1 Journal Article in ANALYTICAL BIOCHEMISTRY

A-Protein gene of bacteriophage MS2

Walther FiersRoland ContrerasFred DuerinckGuy HaegemanJozef MerregaertWilly Min JouAlex RaeymakersGuido VolckaertMarc YsebaertJoël Vandekerckhove et al.

A1 Journal Article in NATURE

Promoter-dependent transcription of tRNAITyr genes using DNA fragments produced by restriction enzymes

Hans KüpperRoland ContrerasArthur LandyH Gobind Khorana

A1 Journal Article in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

1973

Bacteriophage MS2 RNA: nucleotide sequence of the end of the A protein gene and the intercistronic region

Roland ContrerasMarc YsebaertWilly Min JouWalther Fiers

A1 Journal Article in NATURE-NEW BIOLOGY

1972

Studies on the bacteriophage MS2: some nucleotide sequences from the RNA-polymerase gene

Roland ContrerasGuido VolckaertAntoon VandenbergheWalther FiersWilly Min Jou

A1 Journal Article in FEBS LETTERS

1971

A new method for partial digestion useful for sequence analysis of polynucleotides

Roland ContrerasWalther Fiers

A1 Journal Article in FEBS LETTERS

Position of the first initiation codon in the RNA of bacteriophages MS2 and R17

R. De WachterA. VandenbergheJ. MerregaertRoland ContrerasWalther Fiers

Recent progress in the sequence determination of bacteriophage MS2 RNA

Walther FiersRoland ContrerasRupert De WachterGuy HaegemanJozef MerregaertWilly Min JouAntoon Vandenberghe

A1 Journal Article in BIOCHIMIE

Studies on the bacteriophage MS2 nucleotide sequence of a 3′-terminal fragment (n = 104)

Roland ContrerasAntoon VandenbergheWilly Min JouRupert De WachterWalther Fiers

A1 Journal Article in FEBS LETTERS

Studies on the bacteriophage MS2: the untranslated 5'-terminal nucleotide sequence preceding the first cistron

Rupert De WachterJozef MerregaertAntoon VandenbergheRoland ContrerasWalther Fiers

A1 Journal Article in EUROPEAN JOURNAL OF BIOCHEMISTRY

The leader sequence from the 5'-terminus to the A-protein initiation codon in MS2-virus RNA

Rupert De WachterAntoon VandenbergheJozef MerregaertRoland ContrerasWalther Fiers

A1 Journal Article in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

1970

The 3′-terminal nucleotide sequence (n = 16) of bacteriophage MS2 RNA

Willy Min JouRoland ContrerasWalther Fiers

A1 Journal Article in FEBS LETTERS