Research Explorer

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Researcher

Lien De Wannemaeker

Awards & Distinctions

35 Results

2025

ESM2-driven transmembrane domain selection for optimising plant P450s in microbial hosts

Miguel De BlockCamila PontesLien De WannemaekerMiguel RomeroAlfonso ValenciaMarjan De Mey

2024

A cross-bacterial gene expression toolbox : engineering orthogonal transcription to enable predictable genetic engineering in diverse bacterial hosts

Cara DealDaniel Ariza CalvoBrecht De PaepeAntoni PlanasLien De WannemaekerMarjan De Mey

Advancing Engineered Living Materials : integrating filamentous fungi and bacteria for fully biological and functional living materials

Hannelore WilssensLien De WannemaekerMarjan De Mey

Development of a dynamic feedback genetic circuit for efficient membrane protein production in Cereibacter sphaeroides to produce plant metabolites

Carla Gil PiñeiraLien De WannemaekerMarjan De Mey

Leveraging ESM2 embeddings for transmembrane domain selection in the optimisation of plant cytochrome P450 functionality in microbial cell factories

Miguel De BlockCamila PontesLien De WannemaekerMiguel RomeroAlfonso ValenciaMarjan De Mey

Towards a rational approach to promoter engineering : understanding the complexity of transcription initiation in prokaryotes

Cara DealLien De WannemaekerMarjan De Mey

A1 Journal Article in FEMS MICROBIOLOGY REVIEWS

Towards a universal, predictable and tuneable gene expression toolbox : strategies for engineering orthogonal transcription in bacteria

Cara DealDaniel Ariza CalvoBrecht De PaepeAntoni PlanasLien De WannemaekerMarjan De Mey

Viable co-cultivation of filamentous fungi and engineered bacteria for fully biological and tuneable living materials

Hannelore WilssensLien De WannemaekerMarjan De Mey

2023

Engineering orthogonal transcription for the development of a cross-bacterial, orthogonal and tuneable gene expression toolbox

Cara DealLien De WannemaekerBrecht De PaepeAntoni PlanasMarjan De Mey

Standardization of fluorescent reporter assays in synthetic biology across the visible light spectrum

Lien De WannemaekerFriederike MeyIndra BervoetsMichiel Ver CruysseGeoff S. BaldwinMarjan De Mey

A1 Journal Article in ACS SYNTHETIC BIOLOGY

Taming CRISPRi : dynamic range tuning through guide RNA diversion

Bob Van HoveLien De WannemaekerIsolde MissiaenJo MaertensMarjan De Mey

A1 Journal Article in NEW BIOTECHNOLOGY

2022

A cross-bacterial, orthogonal and tuneable gene expression toolbox for development of microbial cell factories in diverse bacterial hosts

Cara DealLien De WannemaekerBrecht De PaepeAntoni PlanasMarjan De Mey

A universal and orthogonal gene expression system enabling standardized translation initiation across the bacterial domain

Lien De WannemaekerJo MaertensBervoets IndraMarjan De Mey

A universal and orthogonal translation initiation module enabling standardized gene expression across the bacterial domain

Lien De WannemaekerJo MaertensIndra BervoetsMarjan De Mey

Biosensor-driven, model-based optimization of the orthogonally expressed naringenin biosynthesis pathway

Maarten Van BremptAndries PeetersDries DuchiLien De WannemaekerJo MaertensBrecht De PaepeMarjan De Mey

A1 Journal Article in MICROBIAL CELL FACTORIES

Dynamic feedback regulation for efficient membrane protein production using a small RNA-based genetic circuit in Escherichia coli

Chiara GuidiLien De WannemaekerJasmine De BaetsWouter DemeesterJo MaertensBrecht De PaepeMarjan De Mey

A1 Journal Article in MICROBIAL CELL FACTORIES

The cross-bacterial expression system : development of parts and tools for standardized gene engineering across the bacterial domain

Lien De WannemaekerMarjan De MeyJo MaertensIndra Bervoets

Unlocking the bacterial domain for industrial biotechnology applications using universal parts and tools

Lien De WannemaekerIndra BervoetsMarjan De Mey

A1 Journal Article in BIOTECHNOLOGY ADVANCES

Unlocking the potential of non-model bacteria : a cross-bacterial gene expression toolbox

Cara DealLien De WannemaekerBrecht De PaepeAntoni PlanasMarjan De Mey

2020

Countering inner-membrane stress for metabolic engineering applications

Chiara GuidiLien De WannemaekerWouter DemeesterJasmine De BaetsJo MaertensMarjan De Mey

Evaluating inner-membrane stress in E. coli for overexpression of functional membrane proteins

Chiara GuidiLien De WannemaekerWouter DemeesterJasmine De BaetsJo MaertensMarjan De Mey

Synthetic biology tools for the efficient and functional expression of membrane proteins

Chiara GuidiLien De WannemaekerJasmine De BaetsDries DuchiJo MaertensMarjan De Mey

Unlocking the bacterial domain for industrial biotechnology applications using orthogonal parts and tools

Lien De WannemaekerMonica TexidoBéné AmeryJo MaertensIndra BervoetsMarjan De Mey

2019

An orthogonal synthetic biology toolbox for the optimisation of microbial cell factories

Maarten Van BremptAndries PeetersLien De WannemaekerDries DuchiJo MaertensMarjan De Mey

Countering membrane stress for the optimisation of microbial cell factories

Chiara GuidiLien De WannemaekerWouter DemeesterJo MaertensMarjan De Mey

Development of a cross-bacterial expression system : the orthogonal translational module

Lien De WannemaekerJo MaertensMarjan De Mey

Hacking translation : development of orthogonal ribosomes and 5' UTRs allowing standardized gene expression across the bacterial domain

Lien De WannemaekerJo MaertensMarjan De Mey

Repurposing inner-membrane stress for metabolic engineering applications

Chiara GuidiLien De WannemaekerWouter DemeesterJo MaertensMarjan De Mey

2018

Development of N-acetylneuraminic acid responsive biosensors based on the transcriptional regulator NanR

Gert PetersBrecht De PaepeLien De WannemaekerDries DuchiJo MaertensJeroen LammertynMarjan De Mey

A1 Journal Article in BIOTECHNOLOGY AND BIOENGINEERING

Evaluating inner-membrane stress in E. coli

Chiara GuidiLien De WannemaekerJo MaertensMarjan De Mey

Towards orthogonal and standardized translation across the bacterial domain

Lien De WannemaekerJo MaertensMarjan De Mey

2017

Recursive DNA assembly using protected oligonucleotide duplex assisted cloning (PODAC)

Bob Van HoveChiara GuidiLien De WannemaekerJo MaertensMarjan De Mey

A1 Journal Article in ACS SYNTHETIC BIOLOGY

2016

Constructing artificial CRISPR guide RNA arrays using methylated oligonucleotides

Bob Van HoveLien De WannemaekerJo MaertensMarjan De Mey

Not just PCR: using modified oligonucleotides for recursive cloning

Bob Van HoveChiara GuidiLien De WannemaekerJo MaertensMarjan De Mey

Recursive cloning for the assembly of biosynthetic pathways and artificial CRISPR arrays

Bob Van HoveLien De WannemaekerChiara GuidiJo MaertensMarjan De Mey