Research Explorer

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Researcher

Koen Beerens

Awards & Distinctions

52 Results

2025

Exploration and engineering of dehydratases and epimerases for the biocatalytic synthesis of nucleotide diphosphate deoxy sugars

Ulrike VogelTom DesmetKoen Beerens

Protein flexibility drives sugar rotation and high substrate promiscuity in a GDP-sugar 4-epimerase

Carlos Alvarez QuispeKoen BeerensAndy-Mark W.H. ThunnissenXevi BiarnésAntoni PlanasTom Desmet

A1 Journal Article in COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL

2024

Exploring and engineering the substrate specificity of glycoside hydrolases and phosphorylases

Emma De BeulTom DesmetKoen Beerens

Fructosyl amino oxidase as a therapeutic enzyme in age-related macular degeneration

Joris DelangheJosé Diana Di MavunguKoen BeerensJonas HimpeNezahat BostanMarijn SpeeckaertHenk VrielinckAnne VralCaroline van den BroekeManon Huizing et al.

A1 Journal Article in INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

2023

Glycosidic linkage of rare and new-to-nature disaccharides reshapes gut microbiota in vitro

Stanley Omondi OnyangoKoen BeerensQiqiong LiJohn Van CampTom DesmetTom Van de Wiele

A1 Journal Article in FOOD CHEMISTRY

Rare sugar metabolism and impact on insulin sensitivity along the gut-liver-muscle axis in vitro

Amar Daniël Emanuel Van LaarCharlotte GrootaertAndreja RajkovicTom DesmetKoen BeerensJohn Van Camp

A1 Journal Article in NUTRIENTS

The conversion of UDP-Glc to UDP-Man : in silico and biochemical exploration to improve the catalytic efficiency of CDP-tyvelose C2-epimerases

Ulrike VogelMatthieu Da CostaCarlos Alvarez QuispeRobin StragierHenk‐Jan JoostenKoen BeerensTom Desmet

A1 Journal Article in CHEMBIOCHEM

Topical application of deglycating enzymes as an alternative non-invasive treatment for presbyopia

Joris DelangheJeroen BeeckmanKoen BeerensJonas HimpeNezahat BostanMarijn SpeeckaertMargo NotebaertManon HuizingElisabeth Van Aken

A1 Journal Article in INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

2022

A colorimetric assay for the screening and kinetic analysis of nucleotide sugar 4,6-dehydratases

Ulrike VogelKoen BeerensTom Desmet

A1 Journal Article in ANALYTICAL BIOCHEMISTRY

Exploration of archaeal nucleotide sugar epimerases unveils a new and highly promiscuous GDP-Gal4E subgroup

Carlos Alvarez QuispeMatthieu Da CostaKoen BeerensTom Desmet

A2 Journal Article in CURRENT RESEARCH IN BIOTECHNOLOGY

GDP-mannose 3,5-epimerase : a view on structure, mechanism, and industrial potential

Koen BeerensOphelia GevaertTom Desmet

A1 Journal Article in FRONTIERS IN MOLECULAR BIOSCIENCES

Metabolism and health effects of rare sugars in a CACO-2/HepG2 coculture model

Amar Daniël Emanuel Van LaarCharlotte GrootaertFilip Van NieuwerburghDieter DeforceTom DesmetKoen BeerensJohn Van Camp

A1 Journal Article in NUTRIENTS

Nucleotide sugar dehydratases : structure, mechanism, substrate specificity, and application potential

Ulrike VogelKoen BeerensTom Desmet

A1 Journal Article in JOURNAL OF BIOLOGICAL CHEMISTRY

Sweet biotechnology : enzymatic production and digestibility screening of novel kojibiose and nigerose analogues

Shari DhaeneAmar Daniël Emanuel Van LaarMarc De DonckerEmma De BeulKoen BeerensCharlotte GrootaertJurgen CaroenJohan Van der EyckenJohn Van CampTom Desmet

A1 Journal Article in JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

2021

Defined synthesis of cellotriose by cellobiose phosphorylase mutant

Zorica UbiparipDavid SáezKoen BeerensTom Desmet

Discovery of a novel GDP-glucose 4-epimerase in archaea reveals an uncommon promiscuity

Carlos Alvarez QuispeMatthieu Da CostaKoen BeerensTom Desmet

Engineering of a thermostable biocatalyst for the synthesis of 2-O-glucosylglycerol

Jorick FranceusZorica UbiparipKoen BeerensTom Desmet

A1 Journal Article in CHEMBIOCHEM

Engineering of cellobiose phosphorylase for the synthesis of prebiotic cellotriose

Zorica UbiparipDavid Sáez MorenoKoen BeerensTom Desmet

Expanding the enzyme repertoire for sugar nucleotide epimerization : the CDP-tyvelose 2-epimerase from Thermodesulfatator atlanticus for glucose/mannose interconversion

Christian RappStevie Van OvertveldtKoen BeerensHansjörg WeberTom DesmetBernd Nidetzky

A1 Journal Article in APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Exploration of GH94 sequence space for enzyme discovery reveals a novel glucosylgalactose phosphorylase specificity

Marc De DonckerChloé De GraeveJorick FranceusKoen BeerensVladimír KřenHelena PelantováRonny VercauterenTom Desmet

A1 Journal Article in CHEMBIOCHEM

Kojibiose and trehalose long-term supplementation reveals significant metabolic differences and disaccharide dependent microbial community shift in a human gastrointestinal model

Stanley Omondi OnyangoKoen BeerensXinlin JiaoTom DesmetTom Van de Wiele

Stereo-electronic control of reaction selectivity in short-chain dehydrogenases : decarboxylation, epimerization, and dehydration

Annika J.E. BorgKoen BeerensMartin PfeifferTom DesmetBernd Nidetzky

A1 Journal Article in CURRENT OPINION IN CHEMICAL BIOLOGY

Structure-function relationships in NDP-sugar active SDR enzymes : fingerprints for functional annotation and enzyme engineering

Structure-function relationships in NDP-sugar active SDR enzymes : fingerprints for functional annotation and enzyme engineering

Matthieu Da CostaOphelia GevaertStevie Van OvertveldtJoanna LangeHenk-Jan JoostenTom DesmetKoen Beerens

A1 Journal Article in BIOTECHNOLOGY ADVANCES

Towards sustainable production of defined oligosaccharides : enzymatic synthesis of high purity cellotriose

Zorica UbiparipDavid Sáez MorenoKoen BeerensTom Desmet

β-Glucan phosphorylases in carbohydrate synthesis

Zorica UbiparipMarc De DonckerKoen BeerensJorick FranceusTom Desmet

A1 Journal Article in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

2020

Determinants of the nucleotide specificity in the carbohydrate epimerase family 1

Stevie Van OvertveldtMatthieu Da CostaOphelia GevaertHenk-Jan JoostenKoen BeerensTom Desmet

A1 Journal Article in BIOTECHNOLOGY JOURNAL

Engineering of cellobiose phosphorylase for the defined synthesis of cellotriose

Zorica UbiparipDavid Sáez MorenoKoen BeerensTom Desmet

A1 Journal Article in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Fermentation of rare sugar kojibiose by the oral bacteria is slow yet bacteria specific

Stanley Omondi OnyangoNele De ClercqKoen BeerensTom DesmetTom Van de Wiele

GDP-altrose as novel product of GDP-mannose 3,5-epimerase : revisiting its reaction mechanism

Ophelia GevaertStevie Van OvertveldtMatthieu Da CostaKoen BeerensTom Desmet

A1 Journal Article in INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Novel insights into the existence of the putative UDP-glucuronate 5-epimerase specificity

Ophelia GevaertStevie Van OvertveldtMatthieu Da CostaKoen BeerensTom Desmet

A1 Journal Article in CATALYSTS

Oral microbiota display profound differential metabolic kinetics and community shifts upon incubation with sucrose, trehalose, kojibiose, and xylitol

Stanley Omondi OnyangoNele De ClercqKoen BeerensJohn Van CampTom DesmetTom Van de Wiele

A1 Journal Article in APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Synthesis, trehalase hydrolytic resistance and inhibition properties of 4-and 6-substituted trehalose derivatives

Shari DhaeneJohan Van der EyckenKoen BeerensJorick FranceusTom DesmetJurgen Caroen

A1 Journal Article in JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY

2019

Characterization of the first bacterial and thermostable GDP-mannose 3,5-epimerase

Ophelia GevaertStevie Van OvertveldtKoen BeerensTom Desmet

A1 Journal Article in INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Discovery of novel cellodextrin phosphorylases : where bioinformatics meets biochemistry

Zorica UbiparipJorick FranceusNídia MartinsKoen BeerensTom Desmet

Rational design of an improved transglucosylase for production of the rare sugar nigerose

Jorick FranceusShari DhaeneHannes DecadtJudith VandepitteJurgen CaroenJohan Van der EyckenKoen BeerensTom Desmet

A1 Journal Article in CHEMICAL COMMUNICATIONS

2018

Converting galactose into the rare sugar talose with cellobiose 2-epimerase as biocatalyst

Stevie Van OvertveldtOphelia GevaertMartijn CherletKoen BeerensTom Desmet

A1 Journal Article in MOLECULES

Evolutionary analysis as a powerful complement to energy calculations for protein stabilization

Koen BeerensStanislav MazurenkoAntonin KunkaSergio M. MarquesNiels HansenMilos MusilRadka ChaloupkovaJitka WatermanJan BrezovskyDavid Bednar et al.

A1 Journal Article in ACS CATALYSIS

Molecular gating of an engineered enzyme captured in real time

Piia KokkonenJan SykoraZbynek ProkopAvisek GhoseDavid BednarMariana AmaroKoen BeerensSarka BidmanovaMichaela SlanskaJan Brezovsky et al.

A1 Journal Article in JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Thermostable alpha-glucan phosphorylases : characteristics and industrial applications

Zorica UbiparipKoen BeerensJorick FranceusRonny VercauterenTom Desmet

A1 Journal Article in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

2017

Biocatalytic synthesis of the rare sugar kojibiose : process scale-up and application testing

Koen BeerensKarel De WinterDavy Van de WalleCharlotte GrootaertSenem KamilogluLisa MiclotteTom Van de WieleJohn Van CampKoen DewettinckTom Desmet

A1 Journal Article in JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Disulfide bridges as essential elements for the thermostability of lytic polysaccharide monooxygenase LPMO10C from Streptomyces coelicolor

Magali TangheBarbara DanneelsMatthias LastKoen BeerensIngeborg StalsTom Desmet

A1 Journal Article in PROTEIN ENGINEERING DESIGN & SELECTION

Exploration of protein unfolding by modelling calorimetry data from reheating

Stanislav MazurenkoAntonin KunkaKoen BeerensChristopher M. JohnsonJiri DamborskyZbynek Prokop

A1 Journal Article in SCIENTIFIC REPORTS

The 'epimerring' highlights the potential of carbohydrate epimerases for rare sugar production

Koen BeerensStevie Van OvertveldtTom Desmet

A1 Journal Article in BIOCATALYSIS AND BIOTRANSFORMATION

2016

Stability analysis of a plant glycosyltransferase

Griet DewitteKoen BeerensMargo DiricksTom Desmet

2015

A structural classification of carbohydrate epimerases : from mechanistic insights to practical applications

Stevie Van OvertveldtTom VerhaegheHenk-Jan JoostenTom van den BerghKoen BeerensTom Desmet

A1 Journal Article in BIOTECHNOLOGY ADVANCES

FireProt : energy- and evolution-based computational design of thermostable multiple-point mutants

David BednarKoen BeerensEva SebestovaJaroslav BendlSagar KhareRadka ChaloupkovaZbynek ProkopJan BrezovskyDavid BakerJiri Damborsky

A1 Journal Article in PLOS COMPUTATIONAL BIOLOGY

Site-specific analysis of protein hydration based on unnatural amino acid fluorescence

Mariana AmaroJan BrezovskýSilvia KováčováJan SýkoraDavid BednářVáclav NěmecVeronika LiškováNagendra Prasad KurumbangKoen BeerensRadka Chaloupková et al.

A1 Journal Article in JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

UDP-hexose 4-epimerases: a view on structure, mechanism and substrate specificity

Koen BeerensWim SoetaertTom Desmet

A1 Journal Article in CARBOHYDRATE RESEARCH

2013

Characterization and engineering of epimerases for the production of rare sugars

Koen BeerensWim SoetaertTom Desmet

Characterization and mutational analysis of the UDP-Glc(NAc) 4-epimerase from Marinithermus hydrothermalis

Koen BeerensWim SoetaertTom Desmet

A1 Journal Article in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

2012

Enzymes for the biocatalytic production of rare sugars

Koen BeerensTom DesmetWim Soetaert

A1 Journal Article in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY