Research Explorer

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Researcher

Juan Fraire

Awards & Distinctions

39 Results

2025

Gentle and efficient engineering of primary human NK cells by photoporation with polydopamine nanosensitizers

Charlotte HinnekensJana RamonM. BirbenW.T.V. GermeraadA. HarizajMargo De VelderStefaan De SmedtBart VandekerckhoveKevin BraeckmansJuan Fraire

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

2024

Non-viral engineering of natural killer cells : exploring nanoparticle-sensitized photoporation as a promising transfection technology

Charlotte HinnekensKevin BraeckmansJuan Fraire

2023

Immunotherapy : towards engineered NK cell therapy : mRNA transfection of NK92 cells by photoporation with biodegradable polydopamine nanosensitizers

Charlotte HinnekensAranit HarizajIlia GoemaereStefaan De SmedtBart VandekerckhoveJuan FraireKevin Braeckmans

Laser‐scanning microscopy for electrophoretic mobility characterization of single nanoparticles

Lucas OorlynckYera UssembayevIngrid Amer CidJuan FraireCharlotte HinnekensKevin BraeckmansFilip Strubbe

A1 Journal Article in PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION

Light-triggered mechanical disruption of extracellular barriers by swarms of enzyme-powered nanomotors for enhanced delivery

Juan FraireMaria GuixAna C. HortelaoNoelia Ruiz-GonzálezAnna C. BakeneckerPouria RamezaniCharlotte HinnekensFélix SauvageStefaan De SmedtKevin Braeckmans et al.

A1 Journal Article in ACS NANO

Non-viral engineering of NK cells

Charlotte HinnekensStefaan De SmedtJuan FraireKevin Braeckmans

A1 Journal Article in BIOTECHNOLOGY ADVANCES

Photothermal nanomaterial-mediated photoporation

Ranhua XiongFélix SauvageJuan FraireChaobo HuangStefaan De SmedtKevin Braeckmans

A1 Journal Article in ACCOUNTS OF CHEMICAL RESEARCH

2022

Fast laser scanning imaging for single nanoparticle analysis

Lucas OorlynckYera UssembayevJuan FraireCharlotte HinnekensKevin BraeckmansFilip Strubbe

Gene therapy to enhance angiogenesis in chronic wounds

Elnaz Shaabani SichaniMaryam SharifiaghdamReza Faridi-MajidiStefaan De SmedtKevin BraeckmansJuan Fraire

A1 Journal Article in MOLECULAR THERAPY-NUCLEIC ACIDS

LBL delivery systems for intracellular delivery of therapeutic nucleic acids

Maryam SharifiaghdamJuan FraireKevin Braeckmans

Laser-induced nanobubbles safely ablate vitreous opacities in vivo

Félix SauvageVan Phuc NguyenYanxiu LiAranit HarizajJ. SebagDimitri RoelsViktor Van HavereKaren PeynshaertRanhua XiongJuan Fraire et al.

A1 Journal Article in NATURE NANOTECHNOLOGY

Laser-scanning imaging and electrophoresis of single nanoparticles

Filip StrubbeLucas OorlynckYera UssembayevJuan FraireCharlotte HinnekensKevin Braeckmans

Layer-by-Layer self-assembly technique : a tunable tool for delivery of therapeutic nucleic acids

Elnaz Shaabani SichaniJuan FraireKevin Braeckmans

Light triggered nanoscale biolistics for efficient intracellular delivery of functional macromolecules in mammalian cells

Juan FraireElnaz Shaabani SichaniMaryam SharifiaghdamMatthias RombautCharlotte HinnekensDawei HuaJana RamonLaurens RaesEduardo Bolea FernandezToon Brans et al.

A1 Journal Article in NATURE COMMUNICATIONS

Transient nuclear lamin A/C accretion aids in recovery from vapor nanobubble-induced permeabilisation of the plasma membrane

Gaëlle HouthaeveGerardo García-Díaz BarrigaStephan StremerschHerlinde De KeersmaeckerJuan FraireJo VandesompelePieter MestdaghStefaan De SmedtKevin BraeckmansWinnok De Vos

A1 Journal Article in CELLULAR AND MOLECULAR LIFE SCIENCES

2021

Black phosphorus mediated photoporation : a broad absorption nanoplatform for intracellular delivery of macromolecules

Jielin WangAranit HarizajYongbo WuXiaofang JiangToon BransJuan FraireJulián Mejía MoralesStefaan De SmedtZhilie TangRanhua Xiong et al.

A1 Journal Article in NANOSCALE

Carbon quantum dots as a dual platform for the inhibition and light-based destruction of collagen fibers : implications for the treatment of eye floaters

Alexandre BarrasFélix SauvageInès De HoonKevin BraeckmansDawei HuaGaëtan BuvatJuan FraireChristophe LethienJ. SebagMichael Harrington et al.

A1 Journal Article in NANOSCALE HORIZONS

Cas9 RNP transfection by vapor nanobubble photoporation for ex vivo cell engineering

Laurens RaesMelissa PilleAranit HarizajGlenn GoetgelukJelter Van HoeckStephan StremerschJuan FraireToon BransOlivier Gerrit de JongRoel Maas-Bakker et al.

A1 Journal Article in MOLECULAR THERAPY-NUCLEIC ACIDS

Cytosolic delivery of gadolinium via photoporation enables improved in vivo magnetic resonance imaging of cancer cells

Aranit HarizajBenedicte DescampsChristophe MangodtStephan StremerschArianna StoppaLieve BalcaenToon BransHilde De RoosterNausikaa DevriendtJuan Fraire et al.

A1 Journal Article in BIOMATERIALS SCIENCE

Enhanced siRNA delivery and selective apoptosis induction in H1299 cancer cells by layer-by-layer-assembled Se nanocomplexes : toward more efficient cancer therapy

Maryam SharifiaghdamElnaz Shaabani SichaniZeynab SharifiaghdamHerlinde De KeersmaeckerRiet De RyckeStefaan De SmedtReza Faridi-MajidiKevin BraeckmansJuan Fraire

A1 Journal Article in FRONTIERS IN MOLECULAR BIOSCIENCES

Increasing angiogenesis factors in hypoxic diabetic wound conditions by siRNA delivery : additive effect of LbL-gold nanocarriers and desloratadine-induced lysosomal escape

Elnaz Shaabani SichaniMaryam SharifiaghdamJoris LammensHerlinde De KeersmaeckerChris VervaetThomas De BeerElahe MotevaseliMohammad Hossein GhahremaniParvin MansouriStefaan De Smedt et al.

A1 Journal Article in INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Layer by layer assembled chitosan-coated gold nanoparticles for enhanced siRNA delivery and silencing

Elnaz Shaabani SichaniMaryam SharifiaghdamHerlinde De KeersmaeckerRiet De RyckeStefaan De SmedtReza Faridi-MajidiKevin BraeckmansJuan Fraire

A1 Journal Article in INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Macrophage reprogramming into a pro-healing phenotype by siRNA delivered with LBL assembled nanocomplexes for wound healing applications

Maryam SharifiaghdamElnaz Shaabani SichaniZeynab SharifiaghdamHerlinde De KeersmaeckerBart LucasJoris LammensHossein GhanbariLadan Teimoori-ToolabiChris VervaetThomas De Beer et al.

A1 Journal Article in NANOSCALE

Nanoparticle-sensitized photoporation enables inflammasome activation studies in targeted single cells dagger

Aranit HarizajFilip Van HauwermeirenStephan StremerschRiet De RyckeHerlinde De KeersmaeckerToon BransJuan FraireKarolien GrauwenStefaan De SmedtIne Lentacker et al.

A1 Journal Article in NANOSCALE

Photothermal nanofibres enable safe engineering of therapeutic cells

Ranhua XiongDawei HuaJelter Van HoeckDominika BerdeckaLaurens LégerStijn De MunterJuan FraireLaurens RaesAranit HarizajFélix Sauvage et al.

A1 Journal Article in NATURE NANOTECHNOLOGY

2020

Improving antibiotics' penetration and efficiency for treating biofilm infections by laser-induced vapor nanobubbles

Eline TeirlinckRanhua XiongToon BransKatrien ForierJuan FraireHeleen Van AckerStefaan De SmedtTom CoenyeKevin Braeckmans

Intracellular delivery of mRNA in adherent and suspension cells by vapor nanobubble photoporation

Laurens RaesStephan StremerschJuan FraireToon BransGlenn GoetgelukStijn De MunterLien Van HoeckeRein VerbekeJelter Van HoeckRanhua Xiong et al.

A1 Journal Article in NANO-MICRO LETTERS

Intracellular labeling with extrinsic probes : delivery strategies and applications

Jing LiuJuan FraireStefaan De SmedtRanhua XiongKevin Braeckmans

A1 Journal Article in SMALL

Vapor nanobubble is the more reliable photothermal mechanism for inducing endosomal escape of siRNA without disturbing cell homeostasis

Juan FraireGaëlle HouthaeveJing LiuLaurens RaesLotte VermeulenStephan StremerschToon BransGerardo Garcia-Diaz BarrigaSarah De KeulenaerFilip Van Nieuwerburgh et al.

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

2019

Delivery of mixed-lineage kinase domain-like protein by vapor nanobubble photoporation induces necroptotic-like cell death in tumor cells

Lien Van HoeckeLaurens RaesStephan StremerschToon BransJuan FraireRia RoelandtWim DeclercqPeter VandenabeeleKoen RaemdonckKevin Braeckmans et al.

A1 Journal Article in INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Exploring light-sensitive nanocarriers for simultaneous triggered antibiotic release and disruption of biofilms upon generation of laser-induced vapor nanobubbles

Eline TeirlinckAlexandre BarrasJing LiuJuan FraireTatu LajunenRanhua XiongKatrien ForierChengnan LiArto UrttiRabah Boukherroub et al.

A1 Journal Article in PHARMACEUTICS

Gold nanoparticle-mediated photoporation enables delivery of macromolecules over a wide range of molecular weights in human CD4+ T cells

Laurens RaesClarissa Van HeckeJulie MichielsStephan StremerschJuan FraireToon BransRanhua XiongStefaan De SmedtLinos VandekerckhoveKoen Raemdonck et al.

A1 Journal Article in CRYSTALS

Improved label-free identification of individual exosome-like vesicles with Au@Ag nanoparticles as SERS substrate

Juan FraireStephan StremerschDavinia BouckaertTinne MonteyneThomas De BeerPieter WuytensRiet De RyckeAndre SkirtachKoen RaemdonckStefaan De Smedt et al.

A1 Journal Article in ACS APPLIED MATERIALS & INTERFACES

Laser-induced vapor nanobubbles improve diffusion in biofilms of antimicrobial agents for wound care

Eline TeirlinckJuan FraireHeleen Van AckerJasper WilleRosalie SwimbergheToon BransRanhua XiongMaarten MeireRoeland De MoorStefaan De Smedt et al.

A2 Journal Article in BIOFILM

Mechanistic profiling of the release kinetics of siRNA from lipidoid-polymer hybrid nanoparticles in vitro and in vivo after pulmonary administration

Kaushik ThankiDelphine van EetveldeAntonia GeyerJuan FraireRemi HendrixHannelore Van EygenEmma PuttemanHaider SamiCristiane de Souza Carvalho-WodarzHenrik Franzyk et al.

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Photoablation of human vitreous opacities by light-induced vapor nanobubbles

Félix SauvageJuan FraireKatrien RemautJ SebagKaren PeynshaertMichael HarringtonFrans J Van de VeldeRanhua XiongMarie-Jose TassignonToon Brans et al.

A1 Journal Article in ACS NANO

2018

Laser-induced vapour nanobubbles improve drug diffusion and efficiency in bacterial biofilms

Eline TeirlinckRanhua XiongToon BransKatrien ForierJuan FraireHeleen Van AckerNele MatthijsRiet De RyckeStefaan De SmedtTom Coenye et al.

A1 Journal Article in NATURE COMMUNICATIONS

Photothermally triggered endosomal escape and its influence on transfection efficiency of gold-functionalized JetPEI/pDNA nanoparticles

Lotte VermeulenJuan FraireLaurens RaesEllen De MeesterSarah De KeulenaerFilip Van NieuwerburghStefaan De SmedtKatrien RemautKevin Braeckmans

A1 Journal Article in INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy

Jing LiuRanhua XiongToon BransSaskia LippensEef ParthoensFrancesca Cella ZanacchiRaffaella MagrassiSantosh K SinghSreekumar KurungotSabine Szunerits et al.

A1 Journal Article in LIGHT-SCIENCE & APPLICATIONS