Research Explorer

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Researcher

Dirk de Graaf

Publications & Research Data

Awards & Distinctions

214 Results

2026

Evaluation of 10-year selection for virus resistance in a mass breeding program

Emma BossuytMarleen BrunainLina De SmetEllen DanneelsDirk de Graaf

A1 Journal Article in INSECTS

Performances of selective mechanical traps for autumn control of the invasive Asian hornet Vespa velutina nigrithorax in Western and Southern Europe

Camilla PedrelliMarco PietropaoliStefano PalombaCarlos Marín BarcáizteguiMaria Shantal Rodríguez FloresEster OrdóñezMartina GirolaDirk de GraafGiovanni Formato

A1 Journal Article in APPLIED SCIENCES-BASEL

Synthetic varroacides in honey bee colonies : a comprehensive monitoring program across the European Union

Jozef J.M. Van der SteenRobert BrodschneiderValters BrusbardisBas BuddendorNorman CarreckEllen DanneelsDirk de GraafKristina GratzerAlison GrayFani Hatjina et al.

A1 Journal Article in ENVIRONMENTAL TECHNOLOGY & INNOVATION

The diversity of insecticide target site mutations in Apis mellifera

Gilles VerbinnenLina De SmetDora HenriquesJan TytgatM. Alice PintoDirk de Graaf

A1 Journal Article in BMC GENOMICS

Unlocking Ethiopian beekeeping potential through production systems and value chain analysis : the case of Southwest Ethiopia

Mulubrihan Bayissa TulluWim VerbekeLudwig LauwersDirk de GraafFikadu Mitiku Abdissa

2025

Age and season-dependent dynamics of pathogens and hemocytes in honey bees

Cato Van HerzeleHans NauwynckDirk de Graaf

Challenges for improved production and value share along the honey value chain in Ethiopia

Mulubrihan Bayissa TulluLudwig LauwersFikadu MitikuDirk de GraafWim Verbeke

A1 Journal Article in AGRICULTURE-BASEL

Deformed wing virus affects foraging success and foraging specialization of honeybee workers

Helena Mendes FerreiraKristof BenaetsLina De SmetDirk de GraafTom Wenseleers

A1 Journal Article in SCIENTIFIC REPORTS

Discovery of multiple bee-hazardous pesticides in ornamental plants via the Bee-Plex multi-target microsphere screening method

Rubing ZouRuud van DamNathalie SmitsErik BeijToine BoveeDirk de GraafYirong GuoJeroen Peters

A1 Journal Article in JOURNAL OF HAZARDOUS MATERIALS

Exploration of innovative bee breeding for parasite and virus resistance in Flanders, Belgium

Regis LefebreDirk de GraafLuc Peelman

Exploration of the hemocyte surfaceome of Apis mellifera by a proteomic and transcriptomic approach

Merel BraeckmanLina De SmetBart DevreeseDirk de Graaf

A1 Journal Article in INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Honey bee hives as biomonitors of pesticide environmental pollution : the INSIGNIA-EU monitoring action

Amadeo R. Fernández-AlbaMaría Murcia-MoralesJosé Luis Oller-SerranoJosé Antonio MartínezJozef J.M. Van der SteenRobert BrodschneiderKristina GratzerFani HatjinaNorman CarreckAlison Gray et al.

A1 Journal Article in SCIENCE OF THE TOTAL ENVIRONMENT

Identification and functional genomic analyses of Bartonella isolates from honey bees, and reassessment of the taxonomy of the genus Bartonella

Juliana Botero CardonaNikolas BaslerMargo CnockaertCharlotte PeetersMaria SchreiberManja MarzDirk de GraafJelle MatthijnssensPeter Vandamme

A1 Journal Article in SYSTEMATIC AND APPLIED MICROBIOLOGY

Living in harmony with nature : the key to resilience of honeybees (Apis mellifera) in Africa

Haftom NgussieRegis LefebreDirk de Graaf

A1 Journal Article in APIDOLOGIE

Occurrence and allele frequencies of genetic variants associated with Varroa drone brood resistance (DBR) in African Apis mellifera subspecies

Haftom NgussieRegis LefebreMoses ChemurotFelicien AmakpeBezabeh AmssaluLina De SmetDirk de Graaf

A1 Journal Article in JOURNAL OF INVERTEBRATE PATHOLOGY

Pathogen and hemocyte dynamics in three apiaries across a bee season

Cato Van HerzeleSieglinde CoppensSebastiaan TheunsLina De SmetJolien Van CleemputDirk de GraafHans Nauwynck

A1 Journal Article in TRANSBOUNDARY AND EMERGING DISEASES

Projecting the impacts of climate change on habitat distribution of Varroa destructor in Ethiopia using MaxEnt ecological modeling

Haftom NgussieYikunoamlak GebrewahidGebre HadguDirk de Graaf

A1 Journal Article in SCIENCE OF THE TOTAL ENVIRONMENT

Remote and automated detection of Asian hornets (Vespa velutina nigrithorax) at an apiary, using spectral features of their hovering flight sounds

Harriet HallMartin BencsikNuno CapelaJosé Paulo SousaDirk de Graaf

V Journal Article in COMPUTERS AND ELECTRONICS IN AGRICULTURE

Reviewing published and grey literature in local European languages to supplement existing databases on floral resource traits

Aleksandra WalczyńskaMerel BraeckmanNuno CapelaDirk de GraafDaniel Severus DezmireanYoko L. DupontFernando A. Fleites‐AyilAmelia V. González PortoLinn F. GroeneveldDelphine Jullien et al.

A2 Journal Article in ECOLOGICAL SOLUTIONS AND EVIDENCE

2024

A field realistic model to assess the effects of pesticides residues and adulterants on honey bee gene expression

Noëmie El AgrebiLina De SmetCaroline DounyMarie-Louise ScippoLidija SvečnjakDirk de GraafClaude Saegerman

A1 Journal Article in PLOS ONE

Allele frequencies of genetic variants associated with Varroa drone brood resistance (DBR) in Apis mellifera subspecies across the European continent

Regis LefebreLina De SmetAnja TehelRobert J. PaxtonEmma BossuytWim VerbekeCoby van DooremalenZeynep N. UlgezenTrudy van den BoschFamke Schaafsma et al.

A1 Journal Article in INSECTS

Bridging the gap between field experiments and machine learning : the EC H2020 B-GOOD project as a case study towards automated predictive health monitoring of honey bee colonies

Coby van DooremalenZeynep N. UlgezenRaffaele Dall’OlioUgoline GodeauXiaodong DuanJosé Paulo SousaMarc O. SchäferAlexis BeaurepairePim van GennipMarten Schoonman et al.

A1 Journal Article in INSECTS

Comprehensive approach to phenotype Varroa destructor reproduction in honey bee drone brood and its correlation with decreased mite reproduction (DMR)

Regis LefebreDavid Claeys BoúúaertEmma BossuytLina De SmetMarleen BrunainEllen DanneelsDirk de Graaf

A1 Journal Article in INSECTS

Eggs sampling as an effective tool for identifying the incidence of viruses in honey bees involved in artificial queen rearing

Caio E. C. DominguesLaura ŠimencIvan ToplakDirk de GraafLina De SmetWim VerbekeLuc PeelmanLeticia S. AnsaloniAleš Gregorc

A1 Journal Article in SCIENTIFIC REPORTS

European beekeepers’ interest in digital monitoring technology adoption for improved beehive management

Wim VerbekeMariam Amadou DialloCoby van DooremalenMarten SchoonmanJames H. WilliamsMarie Van EspenMarijke D'HaeseDirk de Graaf

A1 Journal Article in COMPUTERS AND ELECTRONICS IN AGRICULTURE

Known and novel viruses in Belgian honey bees : yearly differences, spatial clustering, and associations with overwintering loss

Ward DeboutteLina De SmetMarleen BrunainNikolas BaslerRiet De RyckeLena SmetsDirk de GraafJelle Matthijnssens

A1 Journal Article in MICROBIOLOGY SPECTRUM

Mechanisms by which honey bees Apis mellifera simensis in Ethiopia cope with pests and pathogens

Haftom NgussieDirk de GraafLina De SmetBezabeh Amssalu

New insights into honey bee viral and bacterial seasonal infection patterns using third-generation nanopore sequencing on honey bee haemolymph

Cato Van HerzeleSieglinde CoppensNick VereeckeSebastiaan TheunsDirk de GraafHans Nauwynck

A1 Journal Article in VETERINARY RESEARCH

Population-wide modelling reveals prospects of marker-assisted selection for parasitic mite resistance in honey bees

Regis LefebreBart BroeckxLina De SmetLuc PeelmanDirk de Graaf

A1 Journal Article in SCIENTIFIC REPORTS

Quantitative assessments of honeybee colony’s response to an artificial vibrational pulse resulting in non-invasive measurements of colony’s overall mobility and restfulness

Martin BencsikAdam McVeighDavid Claeys BoúúaertNuno CapelaFrederick PennyMichael Ian NewtonJosé Paulo SousaDirk de Graaf

A1 Journal Article in SCIENTIFIC REPORTS

Shift in virus composition in honeybees (Apis mellifera) following worldwide invasion by the parasitic mite and virus vector Varroa destructor

Vincent DoubletMelissa A. Y. OddieFanny MondetEva ForsgrenBjørn DahleElisabeth Furuseth-HansenGeoffrey R. WilliamsLina De SmetMyrsini E. NatsopoulouTomás E. Murray et al.

A1 Journal Article in ROYAL SOCIETY OPEN SCIENCE

System mapping of the production and value chain to explore beekeeping potential in Southwest Ethiopia

Mulubrihan Bayissa TulluLudwig LauwersFikadu MitikuDirk de GraafWim Verbeke

A1 Journal Article in INSECTS

The Better-B project aims to restore the resilience of honey bees

A2 Journal Article in BEE WORLD

2023

Beekeeping in Europe facing climate change : a mixed methods study on perceived impacts and the need to adapt according to stakeholders and beekeepers

Marie Van EspenJames H. WilliamsFátima AlvesChristine Yung HungDirk de GraafWim Verbeke

A1 Journal Article in SCIENCE OF THE TOTAL ENVIRONMENT

Can a simple selection program predict winter mortality? Preliminary evaluation of the Flemish honey bee breeding program steers towards selection by exclusion

David Claeys BoúúaertEllen DanneelsValérie VillersMarleen BrunainRegis LefebreLina De SmetDirk de Graaf

A2 Journal Article in BEE WORLD

Convivina is a specialised core gut symbiont of the invasive hornet Vespa velutina

Gowri Amanda HettiarachchiMargo CnockaertMarie JoossensDavid LaureysJessika De ClippeleerNicolas J. VereeckenDenis MichezGuy SmaggheDirk de GraafPeter Vandamme

A1 Journal Article in INSECT MOLECULAR BIOLOGY

Exploring the external environmental drivers of honey bee colony development

Nuno CapelaArtur SarmentoSandra SimõesSara LopesSílvia CastroAntónio Alves da SilvaJoana AlvesYoko L. DupontDirk de GraafJosé Paulo Sousa

A1 Journal Article in DIVERSITY-BASEL

Expression of molecular markers of resilience against Varroa destructor and bee viruses in Ethiopian honey bees (Apis mellifera simensis) focussing on olfactory sensing and the RNA interference machinery

Haftom NgussieDavid Claeys BoúúaertMichel AspergesBezabeh AmssaluLina De SmetDirk de Graaf

A1 Journal Article in INSECTS

Octaaf Van Laere : more than keeper of the Eva Crane Historical Collection

Dirk de GraafOctaaf Van Laere

Opportunities and implications of selection for reduced vertical transmission of viruses in honey bees

David Claeys BoúúaertDirk de GraafLina De Smet

The Historical Collection of Dr. Eva Crane

A2 Journal Article in BEE WORLD

2022

B-GOOD : giving beekeeping guidance by computational-assisted decision making

Dirk de GraafMartin BencsikLina De SmetPeter NeumannMarten SchoonmanJosé Paulo SousaChristopher ToppingWim VerbekeJames WilliamsCoby van Dooremalen

A2 Journal Article in RESEARCH IDEAS AND OUTCOMES

Beekeepers perception of risks affecting colony loss : a pilot survey

Noemie El AgrebiNathalie SteinhauerVeronique RenaultDirk de GraafClaude Saegerman

A1 Journal Article in TRANSBOUNDARY AND EMERGING DISEASES

Cold case : the disappearance of Egypt bee virus, a fourth distinct master strain of deformed wing virus linked to honeybee mortality in 1970's Egypt

Joachim R. de MirandaLaura E. BrettellNor ChejanovskyAnna K. ChildersAnne DalmonWard DeboutteDirk de GraafVincent DoubletHaftom GebremedhnElke Genersch et al.

A1 Journal Article in VIROLOGY JOURNAL

Hemocyten van de honingbij (Apis mellifera), een babylonische spraakverwarring

Cato Van HerzeleHans NauwynckDirk de Graaf

A1 Journal Article in VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT

Virus prevalence in egg samples collected from naturally selected and traditionally managed honey bee colonies across Europe

David Claeys BoúúaertLina De SmetMarleen BrunainBjørn DahleTjeerd BlacquièreAnne DalmonDaniel DezmireanDylan ElenJanja FilipiAlexandru Giurgiu et al.

A1 Journal Article in VIRUSES-BASEL

2021

Breeding for virus resistance and its effects on deformed wing virus infection patterns in honey bee queens

David Claeys BoúúaertLina De SmetDirk de Graaf

A1 Journal Article in VIRUSES-BASEL

CSI pollen : diversity of honey bee collected pollen studied by citizen scientists

Robert BrodschneiderElfriede Kalcher-SommersguterSabrina KuchlingVincent DietemannAlison GrayJanko BožičAndrejs BriedisNorman L. CarreckRobert ChleboKarl Crailsheim et al.

A1 Journal Article in INSECTS

Honey bee exposure scenarios to selected residues through contaminated beeswax

Olivier WilmartAnne LegrèveMarie-Louise ScippoWim ReybroeckBruno UrbainDirk de GraafPieter SpanoghePhilippe DelahautClaude Saegerman

A1 Journal Article in SCIENCE OF THE TOTAL ENVIRONMENT

Pyrethroid target-site resistance mutations in populations of the honey bee parasite Varroa destructor (Acari: Varroidae) from Flanders, Belgium

Spyridon VlogiannitisWim JonckheereDries LagetDirk de GraafJohn VontasThomas Van Leeuwen

A1 Journal Article in EXPERIMENTAL AND APPLIED ACAROLOGY

qPCR assays with dual-labeled probes for genotyping honey bee variants associated with varroa resistance

David Claeys BoúúaertMario Van PouckeLina De SmetWim VerbekeDirk de GraafLuc Peelman

A1 Journal Article in BMC VETERINARY RESEARCH

Reduced nest development of reared Bombus terrestris within apiary dense human-modified landscapes

Ivan MeeusLaurian ParmentierMatti PismanDirk de GraafGuy Smagghe

A1 Journal Article in SCIENTIFIC REPORTS

Risk and protective indicators of beekeeping management practices

Noemie El AgrebiNathalie SteinhauerSimone TosiLaurent LeinartzDirk de GraafClaude Saegerman

A1 Journal Article in SCIENCE OF THE TOTAL ENVIRONMENT

Standard methods for Apis mellifera venom research

Dirk de GraafMarcia Regina Brochetto BragaRusleyd Maria Magalhaes de AbreuSimon BlankChris H. BridtsLuc S. De ClerckBart DevreeseDidier G. EboTimothy J. FerrisMargo M. Hagendorens et al.

A1 Journal Article in JOURNAL OF APICULTURAL RESEARCH

2020

A state-of-the-art metagenomic view on the virome of honey bees (Apis mellifera) in Belgium

Ward DeboutteJelle MatthijnssensDirk de GraafKai Dallmeier

Comparison between Apicystis cryptica sp. n. and Apicystis bombi (Arthrogregarida, Apicomplexa): Gregarine parasites that cause fat body hypertrophism in bees

Karel SchoonvaereMarleen BrunainFemke BaekeMichiel De BruyneRiet De RyckeDirk de Graaf

A1 Journal Article in EUROPEAN JOURNAL OF PROTISTOLOGY

Heritability estimates of the novel trait 'suppressed in ovo virus infection' in honey bees (Apis mellifera)

Dirk de GraafDries LagetLina De SmetDavid Claeys BoúúaertMarleen BrunainRoel F. VeerkampEvert W. Brascamp

A1 Journal Article in SCIENTIFIC REPORTS

Honey bee colony winter loss rates for 35 countries participating in the COLOSS survey for winter 2018-2019, and the effects of a new queen on the risk of colony winter loss

Alison GrayNoureddine AdjlaneAlireza ArabAlexis BallisValters BrusbardisJean-Daniel CharriereRobert ChleboMary F. CoffeyBram CornelissenCristina Amaro da Costa et al.

A1 Journal Article in JOURNAL OF APICULTURAL RESEARCH

Honey-bee-associated prokaryotic viral communities reveal wide viral diversity and a profound metabolic coding potential

Ward DeboutteLeen BellerClaude Kwe YindaPiet MaesDirk de GraafJelle Matthijnssens

A1 Journal Article in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Honeybee and consumer's exposure and risk characterisation to glyphosate-based herbicide (GBH) and its degradation product (AMPA): Residues in beebread, wax, and honey

Noemie El AgrebiSimone TosiOlivier WilmartMarie-Louise ScippoDirk de GraafClaude Saegerman

A1 Journal Article in SCIENCE OF THE TOTAL ENVIRONMENT

Metagenomic approach with the NetoVIR enrichment protocol reveals virus diversity within Ethiopian honey bees (Apis mellifera simensis)

Haftom GebremedhnWard DeboutteKarel SchoonvaerePeter DemaeghtLina De SmetBezabeh AmssaluJelle MatthijnssensDirk de Graaf

A1 Journal Article in VIRUSES-BASEL

Multivariate landscape analysis of honey bee winter mortality in Wallonia, Belgium

Leen Van EschJean-Luc De KokLiliane JanssenBart BuelensLina De SmetDirk de GraafGuy Engelen

A1 Journal Article in ENVIRONMENTAL MODELING & ASSESSMENT

Nationwide screening for bee viruses and parasites in Belgian honey bees

Severine MatthijsValerie De WaeleValerie VandenbergeBenedicte VerhoevenJacqueline EversMarleen BrunainClaude SaegermanPaul J. J. De WinterStefan RoelsDirk de Graaf et al.

A1 Journal Article in VIRUSES-BASEL

Pesticide and veterinary drug residues in Belgian beeswax : occurrence, toxicity, and risk to honey bees

Noemie El AgrebiKirsten TraynorOlivier WilmartSimone TosiLaurent LeinartzEllen DanneelsDirk de GraafClaude Saegerman

A1 Journal Article in SCIENCE OF THE TOTAL ENVIRONMENT

The first detection of Braula coeca in honey bee colonies in Uganda

Moses ChemurotDirk de Graaf

A1 Journal Article in JOURNAL OF APICULTURAL RESEARCH

2019

Belgian case study on flumethrin residues in beeswax : possible impact on honeybee and prediction of the maximum daily intake for consumers

Noemie El AgrebiOlivier WilmartBruno UrbainEllen DanneelsDirk de GraafClaude Saegerman

A1 Journal Article in SCIENCE OF THE TOTAL ENVIRONMENT

Factors restraining the population growth of Varroa destructor in Ethiopian honey bees (Apis mellifera simensis)

Haftom NgussieBezabeh AmssaluLina De SmetDirk de Graaf

A1 Journal Article in PLOS ONE

Honey bee predisposition of resistance to ubiquitous mite infestations

Bart BroeckxLina De SmetTjeerd BlacquièreKevin MaebeMikalaï KhalenkowMario Van PouckeBjorn DahlePeter NeumannKim Bach NguyenGuy Smagghe et al.

A1 Journal Article in SCIENTIFIC REPORTS

Host-microbe interactions between the tsetse fly (Glossina morsitans) and its secondary symbiont Sodalis glossinidius and their impact on trypanosome parasites

Katrien TrappeniersJan Van Den AbbeeleDirk de Graaf

2018

Characterization of native honey bee subspecies in Republic of Benin using morphometric and genetic tools

Felicien AmakpeLina De SmetMarleen BrunainFranciscus JacobsBrice SinsinDirk de Graaf

A1 Journal Article in JOURNAL OF APICULTURAL SCIENCE

Characterization of the honeybee venom proteins C1q-like protein and PVF1 and their allergenic potential

Dennis RusskampMatthias Van VaerenberghStefanie EtzoldBernadette EberleinUlf DarsowMaximilian SchienerLina De SmetMagdalena AbsmaierTilo BiedermannEdzard Spillner et al.

A1 Journal Article in TOXICON

Multi-country loss rates of honey bee colonies during winter 2016/2017 from the COLOSS survey

Robert BrodschneiderAlison GrayNoureddine AdjlaneAlexis BallisValters BrusbardisJean-Daniel CharriereRobert ChleboMary F CoffeyBjorn DahleDirk de Graaf et al.

A1 Journal Article in JOURNAL OF APICULTURAL RESEARCH

Study of the metatranscriptome of eight social and solitary wild bee species reveals novel viruses and bee parasites

Karel SchoonvaereGuy SmaggheFrédéric FrancisDirk de Graaf

A1 Journal Article in FRONTIERS IN MICROBIOLOGY

Unbiased random mutagenesis contributes to a better understanding of the virulent behaviour of Paenibacillus larvae

Tine DescampsLina De SmetPaul De VosDirk de Graaf

A1 Journal Article in JOURNAL OF APPLIED MICROBIOLOGY

2017

A pan-European epidemiological study reveals honey bee colony survival depends on beekeeper education and disease control

Antoine JacquesMarion LaurentMagali Ribiere-ChabertMathilde SaussacStephanie BougeardGiles E BudgePascal HendrikxMarie-Pierre ChauzatDirk de GraafEstelle Meroc et al.

A1 Journal Article in PLOS ONE

Covert deformed wing virus infections have long-term deleterious effects on honeybee foraging and survival

Kristof BenaetsAnneleen Van GeystelenDries CardoenLina De SmetDirk de GraafLiliane SchoofsMaarten HD LarmuseauLaura E BrettellStephen J MartinTom Wenseleers

A1 Journal Article in PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Environmental contaminants of honeybee products in Uganda detected using LC-MS/MS and GC-ECD

Deborah Ruth AmulenPieter SpanogheMichael HoubrakenAndrew TamaleDirk de GraafPaul CrossGuy Smagghe

A1 Journal Article in PLOS ONE

Expression of key components of the RNAi machinery are suppressed in Apis mellifera that suffer a high virus infection

Lina De SmetJorgen RavoetTom WenseleersDirk de Graaf

A1 Journal Article in ENTOMOLOGICAL SCIENCE

Individual and genetic task specialization in policing behaviour in the European honeybee

Ulrich R ErnstDries CardoenVincent CornetteFrancis LW RatnieksDirk de GraafLiliane SchoofsPeter VerleyenTom Wenseleers

A1 Journal Article in ANIMAL BEHAVIOUR

Nosema neumanni n. sp. (Microsporidia, Nosematidae), a new microsporidian parasite of honeybees, Apis mellifera in Uganda

Moses ChemurotLina De SmetMarleen BrunainRiet De RyckeDirk de Graaf

A1 Journal Article in EUROPEAN JOURNAL OF PROTISTOLOGY

Stress indicator gene expression profiles, colony dynamics and tissue development of honey bees exposed to sub-lethal doses of imidacloprid in laboratory and field experiments

Lina De SmetFani HatjinaPavlos IoannidisAnna HamamtzoglouKarel SchoonvaereFrédéric FrancisIvan MeeusGuy SmaggheDirk de Graaf

A1 Journal Article in PLOS ONE

The buzz about bees and poverty alleviation : identifying drivers and barriers of beekeeping in sub-Saharan Africa

Deborah Ruth AmulenMarijke D'HaeseElizabeth AhikirizaJacob Godfrey AgeaFranciscus JacobsDirk de GraafGuy SmagghePaul Cross

A1 Journal Article in PLOS ONE

The distribution, infestation levels and effects of honeybee parasites and pathogens on colony performance in two agro-ecological zones of Uganda

Moses ChemurotDirk de Graaf

The intimate host-pathogen relation of Paenibacillus larvae and the honey bee Apis mellifera

Tine DescampsDirk de GraafPaul De Vos

Towards an increasing honey production in Northern Uganda : a multi-perspective approach

Deborah Ruth AmulenGuy SmaggheDirk de GraafFranciscus Jacobs

Trueness and precision of the real-time RT-PCR method for quantifying the chronic bee paralysis virus genome in bee homogenates evaluated by a comparative inter-laboratory study

Frank SchurrNicolas CougouleMarie-Pierre RiviereMagali Ribiere-ChabertHamid AchourDan AdamCarlos CastilloDirk de GraafEva ForsgrenAnna Granato et al.

A1 Journal Article in JOURNAL OF VIROLOGICAL METHODS

2016

Belgian honey bee winter mortality during 2012-2013 : a case-control study and spatial analysis

Sophie RoelandtEstelle MerocFlavien RiocreuxDirk de GraafKim Bach NguyenMarleen BrunainBenedicte VerhoevenMarc DispasStefan RoelsYves Van der Stede

A1 Journal Article in JOURNAL OF APICULTURAL RESEARCH

C1q-like protein and PVF1 from honeybee venom show IgE-reactivity but do not activate basophils

M SchienerMatthias Van VaerenberghS EtzoldB EberleinLina De SmetM AbsmaierU DarsowT BiedermannE SpillnerM Ollert et al.

Discovery of Lake Sinai virus and an unusual strain of acute bee paralysis virus in West African apiaries

Felicien AmakpeLina De SmetMarleen BrunainJorgen RavoetFranciscus JacobsWim ReybroeckBrice SinsinDirk de Graaf

A1 Journal Article in APIDOLOGIE

Exploring the beekeeping potential in the Republic of Benin by examining the melliferous trees, the honeybees and the honey they produce

Felicien AmakpeDirk de GraafBrice SinsinFranciscus Jacobs

Factors influencing the prevalence and infestation levels of Varroa destructor in honeybee colonies in two highland agro-ecological zones of Uganda

Moses ChemurotAnne M AkolCharles MasembeLina De SmetTine DescampsDirk de Graaf

A1 Journal Article in EXPERIMENTAL AND APPLIED ACAROLOGY

First detection of Paenibacillus larvae the causative agent of American Foulbrood in a Ugandan honeybee colony

Moses ChemurotMarleen BrunainAnne M AkolTine DescampsDirk de Graaf

A1 Journal Article in SPRINGERPLUS

Multiple Locus Variable number of tandem repeat Analysis : a molecular genotyping tool for Paenibacillus larvae

Tine DescampsLina De SmetPieter StragierPaul De VosDirk de Graaf

A1 Journal Article in MICROBIAL BIOTECHNOLOGY

Plasticity in the gut microbial community and uptake of Enterobacteriaceae (Gammaproteobacteria) in Bombus terrestris bumblebees' nests when reared indoors and moved to an outdoor environment

Laurian ParmentierIvan MeeusHadi MosallanejadDirk de GraafGuy Smagghe

A1 Journal Article in APIDOLOGIE

Preliminary analysis of loss rates of honey bee colonies during winter 2015/16 from the COLOSS survey

Robert BrodschneiderAlison GrayRomee van der ZeeNoureddine AdjlaneValters BrusbardisJean-Daniel CharriereRobert ChleboMary F CoffeyKarl CrailsheimBjorn Dahle et al.

A1 Journal Article in JOURNAL OF APICULTURAL RESEARCH

Residues in beeswax : a health risk for the consumer of honey and beeswax?

Olivier WilmartAnne LegreveMarie-Louise ScippoWim ReybroeckBruno UrbainDirk de GraafWalter SteurbautPhilippe DelahautPascal GustinBach Kim Nguyen et al.

A1 Journal Article in JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Risk indicators affecting honeybee colony survival in Europe : one year of surveillance

Marie-Pierre ChauzatAntoine JacquesMarion LaurentStephanie BougeardPascal HendrikxMagali Ribiere-ChabertDirk de GraafEstelle MerocBach Kim NguyenSophie Roelandt et al.

A1 Journal Article in APIDOLOGIE

Track-a-Forager: a program for the automated analysis of RFID tracking data to reconstruct foraging behaviour

A Van GeystelenK BenaetsDirk de GraafMHD LarmuseauT Wenseleers

A1 Journal Article in INSECTES SOCIAUX

Unbiased RNA shotgun metagenomics in social and solitary wild bees detects associations with eukaryote parasites and new viruses

Karel SchoonvaereLina De SmetGuy SmaggheAndy VierstraeteBart BraeckmanDirk de Graaf

A1 Journal Article in PLOS ONE

Varroa destructor Macula-like virus, Lake Sinai virus and other new RNA viruses in wild bumblebee hosts (Bombus pascuorum, Bombus lapidarius and Bombus pratorum)

Laurian ParmentierGuy SmaggheDirk de GraafIvan Meeus

A1 Journal Article in JOURNAL OF INVERTEBRATE PATHOLOGY

2015

Bee pathogens found in Bombus atratus from Colombia : a case study

Viviana GamboaJorgen RavoetMarleen BrunainGuy SmaggheIvan MeeusJudith FigueroaDiego RiañoDirk de Graaf

A1 Journal Article in JOURNAL OF INVERTEBRATE PATHOLOGY

Characterization of two species of Trypanosomatidae from the honey bee Apis mellifera: Crithidia mellificae Langridge and McGhee, 1967 and Lotmaria passim n. gen., n. sp.

Ryan S SchwarzGary R BauchanCharles A MurphyJorgen RavoetDirk de GraafJay D Evans

A1 Journal Article in JOURNAL OF EUKARYOTIC MICROBIOLOGY

Determination of the silvo-melliferous regions of Benin : a nationwide categorisation of the land based on melliferous plants suitable for timber production

Felicien AmakpeGaston AkouehouDirk de GraafBrice Sinsin

A2 Journal Article in JOURNAL OF AGRICULTURE AND RURAL DEVELOPMENT IN THE TROPICS AND SUBTROPICS

Differential diagnosis of the honey bee trypanosomatids Crithidia mellificae and Lotmaria passim

Jorgen RavoetRyan S SchwarzTine DescampsOrlando YañezCansu Ozge TozkarRaquel Martin-HernandezCarolina BartoloméLina De SmetMariano HigesTom Wenseleers et al.

A1 Journal Article in JOURNAL OF INVERTEBRATE PATHOLOGY

Exploring the involvement of honey bee pathogens in colony collapses from epidemiological data, pathogen genotyping and host immune responses

Jorgen RavoetDirk de Graaf

Exploring the potential of venom from Nasonia vitripennis as therapeutic agent with high-throughput screening tools

Ellen DanneelsEllen FormesynDirk de Graaf

A1 Journal Article in TOXINS

Genome sequence heterogeneity of Lake Sinai Virus found in honey bees and Orf1/RdRP-based polymorphisms in a single host

Jorgen RavoetLina De SmetTom WenseleersDirk de Graaf

A1 Journal Article in VIRUS RESEARCH

Honeybee venom proteome profile of queens and winter bees as determined by a mass spectrometric approach

Ellen DanneelsMatthias Van VaerenberghGriet DebyserBart DevreeseDirk de Graaf

A1 Journal Article in TOXINS

IgE recognition of chimeric isoforms of the honeybee (Apis mellifera) venom allergen Api m 10 evaluated by protein array technology

Matthias Van VaerenberghLina De SmetDavid Rafei-ShamsabadiSimon BlankEdzard SpillnerDidier G EboBart DevreeseThilo JakobDirk de Graaf

A1 Journal Article in MOLECULAR IMMUNOLOGY

Pesticides for apicultural and/or agricultural application found in Belgian honey bee wax combs

Jorgen RavoetWim ReybroeckDirk de Graaf

A1 Journal Article in BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY

Potential for spillover of protozoan parasites from domesticated honeybees and reared bumblebees towards wild bumblebees

Jafar MaharramovGuy SmaggheIvan MeeusDirk de Graaf

The genomes of two key bumblebee species with primitive eusocial organization

BM SaddSM BarribeauG BlochDirk de GraafP DeardenCG ElsikJ GadauCJP GrimmelikhuijzenM HasselmannJD Lozier et al.

A1 Journal Article in GENOME BIOLOGY

Unraveling the venom proteome of the bumblebee (Bombus terrestris) by integrating a combinatorial peptide ligand library approach with FT-ICR MS

Matthias Van VaerenberghGriet DebyserGuy SmaggheBart DevreeseDirk de Graaf

A1 Journal Article in TOXICON

Vertical transmission of honey bee viruses in a Belgian queen breeding program

Jorgen RavoetLina De SmetTom WenseleersDirk de Graaf

A1 Journal Article in BMC VETERINARY RESEARCH

2014

Beeswax residue analysis points to an alarming contamination : a Belgian case study

Jorgen RavoetWim ReybroeckLina De SmetDirk de Graaf

Effect of bodily fluids from honey bee (Apis mellifera) larvae on growth and genome-wide transcriptional response of the causal agent of American Foulbrood disease (Paenibacillus larvae)

Lina De SmetDieter De KokerAlyse K HawleyLeonard J FosterPaul De VosDirk de Graaf

A1 Journal Article in PLOS ONE

Effect of oral infection with Kashmir bee virus and Israeli acute paralysis virus on bumblebee (Bombus terrestris) reproductive success

Ivan MeeusJoachim R de MirandaDirk de GraafFelix WäckersGuy Smagghe

A1 Journal Article in JOURNAL OF INVERTEBRATE PATHOLOGY

Exploring the hidden honeybee (Apis mellifera) venom proteome by integrating a combinatorial peptide ligand library approach with FTMS

Matthias Van VaerenberghGriet DebyserBart DevreeseDirk de Graaf

A1 Journal Article in JOURNAL OF PROTEOMICS

Finding the missing honey bee genes: lessons learned from a genome upgrade

Christine G ElsikKim C WorleyAnna K BennettMartin BeyeFrancisco CamaraChristopher P ChildersDirk de GraafGriet DebyserJixin DengBart Devreese et al.

A1 Journal Article in BMC GENOMICS

Gamma irradiation of pollen and eradication of Israeli acute paralysis virus

Ivan MeeusHadi MosallanejadJinzhi NiuDirk de GraafFelix WäckersGuy Smagghe

A1 Journal Article in JOURNAL OF INVERTEBRATE PATHOLOGY

Holistic screening of collapsing honey bee colonies in Spain: a case study

Almudeno CeperoJorgen RavoetTamara Gómez-MorachoJosé Luis BernalMaria J Del NozalCarolina BartoloméXulio MasideAránzazu MeanaAmelia González-PortoDirk de Graaf et al.

A2 Journal Article in BMC RESEARCH NOTES

How the venom from the ectoparasitoid wasp Nasonia vitripennis exhibits anti-inflammatory properties on mammalian cell lines

Ellen DanneelsSarah GerloKaren HeyninckKathleen Van CraenenbroeckKarolien De BosscherGuy HaegemanDirk de Graaf

A1 Journal Article in PLOS ONE

Hydroxylmethylfurfural induces reactive oxygen species (ROS)-dependent activation of the Toll pathway in honey bees

Lina De SmetClaude SaegermanJorgen RavoetDirk de Graaf

In vitro diagnosis of Hymenoptera venom allergy and further development of component resolved diagnostics

Didier G EboMatthias Van VaerenberghDirk de GraafChris H BridtsLuc S De ClerckVito Sabato

A1 Journal Article in EXPERT REVIEW OF CLINICAL IMMUNOLOGY

Reproduction of honeybee workers is regulated by epidermal growth factor receptor signaling

Ellen FormesynDries CardoenUlrich R ErnstEllen DanneelsMatthias Van VaerenberghDieter De KokerPeter VerleyenTom WenseleersLiliane SchoofsDirk de Graaf

A1 Journal Article in GENERAL AND COMPARATIVE ENDOCRINOLOGY

The venom of Nasonia vitripennis : involvement in the host-parasitoid interaction and putative biomedical application

Ellen DanneelsDirk de GraafKathleen Van CraenenbroeckFranciscus Jacobs

Widespread occurrence of honey bee pathogens in solitary bees

Jorgen RavoetLina De SmetIvan MeeusGuy SmaggheTom WenseleersDirk de Graaf

A1 Journal Article in JOURNAL OF INVERTEBRATE PATHOLOGY

2013

Comprehensive bee pathogen screening in Belgium reveals Crithidia mellificae as a new contributory factor to winter mortality

Jorgen RavoetJafar MaharramovIvan MeeusLina De SmetTom WenseleersGuy SmaggheDirk de Graaf

A1 Journal Article in PLOS ONE

Early changes in the pupal transcriptome of the flesh fly Sarcophagha crassipalpis to parasitization by the ectoparasitic wasp, Nasonia vitripennis

Ellen DanneelsEllen FormesynDaniel A HahnDavid L DenlingerDries CardoenTom WenseleersLiliane SchoofsDirk de Graaf

A1 Journal Article in INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Exploring diverse molecular processes in two genome sequenced hymenopteran model systems : Nasonia vitripennis-(non)host interactions and honeybee fertility

Ellen FormesynDirk de Graaf

Extending the honey bee venome with the antimicrobial peptide apidaecin and a protein resembling wasp antigen 5

Matthias Van VaerenberghDries CardoenEllen FormesynMarleen BrunainGonzalez Van DriesscheS BlankE SpillnerPeter VerleyenTom WenseleersLiliane Schoofs et al.

A1 Journal Article in INSECT MOLECULAR BIOLOGY

Genetic variability of the neogregarine Apicystis bombi, an etiological agent of an emergent bumblebee disease

Jafar MaharramovIvan MeeusKevin MaebeMarina ArbetmanCarolina MoralesPeter GraystockWilliam OH HughesSantiago PlischukCarlos E LangeDirk de Graaf et al.

A1 Journal Article in PLOS ONE

Honeybee (Apis mellifera) and bumblebee (Bombus terrestris) venom: analysis and immunological importance of the proteome

Matthias Van VaerenberghDirk de GraafBart Devreese

Hydroxymethylfurfural: a possible emergent cause of honey bee mortality?

Lara ZirbesBach Kim NguyenDirk de GraafBruno De MeulenaerWim ReybroeckEric HaubrugeClaude Saegerman

A1 Journal Article in JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

IgE recognition of multiple novel Api m 10 isoforms evaluated by protein array technology

Matthias Van VaerenberghLina De SmetS BlankE SpillnerD EboT JakobBart DevreeseDirk de Graaf

Standard methods for American foulbrood research

Dirk de GraafAdriana M AlippiKarina AntunezKatherine A AronsteinGiles BudgeDieter De KokerLina De SmetDouglas W DingmanJay D EvansLeonard J Foster et al.

A1 Journal Article in JOURNAL OF APICULTURAL RESEARCH

Standard methods for virus research in Apis mellifera

Joachim R de MirandaLesley BaileyBrenda V BallPhilippe BlanchardGiles E BudgeNor ChejanovskyYan-Ping ChenLaurent GauthierElke GenerschDirk de Graaf et al.

A1 Journal Article in JOURNAL OF APICULTURAL RESEARCH

The role of serine- and metalloproteases in Nasonia vitripennis venom in cell death related processes towards a Spodoptera frugiperda Sf21 cell line

Ellen FormesynKaren HeyninckDirk de Graaf

A1 Journal Article in JOURNAL OF INSECT PHYSIOLOGY

2012

BeeDoctor, a versatile MLPA-based diagnostic tool for screening bee viruses

Lina De SmetJorgen RavoetJoachim R de MirandaTom WenseleersMatthias Y MuellerRobin FA MoritzDirk de Graaf

A1 Journal Article in PLOS ONE

Influence of the uterine environment on the development of in vitro-produced equine embryos

Katrien SmitsJan GovaereLuc PeelmanKaren GoossensDirk de GraafDries VercauterenLeen VandaeleMaarten HoogewijsEline WydoogheTorn Stout et al.

A1 Journal Article in REPRODUCTION

Proteomics of the venom of the parasitoid Nasonia vitripennis

Ellen FormesynEllen DanneelsDirk de Graaf

Bookchapter in Parasitoid viruses : symbionts and pathogens

Worker honeybee sterility: a proteomic analysis of suppressed ovary activation

Dries CardoenUlrich R ErnstBart BoerjanAnnelies BogaertsEllen FormesynDirk de GraafTom WenseleersLiliane SchoofsPeter Verleyen

A1 Journal Article in JOURNAL OF PROTEOME RESEARCH

2011

Analyse du risque posé en santé animale par la présence de l'hydroxyméthylfurfural dans les sirops de nourrissement des abeilles domestiques

O WilmartW ReybroeckBruno De MeulenaerDirk de GraafB NguyenAndré HuyghebaertC Saegerman

A1 Journal Article in ANNALES DE MEDECINE VETERINAIRE

Differential proteomics in dequeened honeybee colonies reveals lower viral load in hemolymph of fertile worker bees

Dries CardoenUlrich R ErnstMatthias Van VaerenberghBart BoerjanDirk de GraafTom WenseleersLiliane SchoofsPeter Verleyen

A1 Journal Article in PLOS ONE

Effects of invasive parasites on bumble bee declines

Ivan MeeusMark JF BrownDirk de GraafGuy Smagghe

A1 Journal Article in CONSERVATION BIOLOGY

Genome-wide analysis of alternative reproductive phenotypes in honeybee workers

Dries CardoenTom WenseleersUlrich R ErnstEllen DanneelsDries LagetDirk de GraafLiliane SchoofsPeter Verleyen

A1 Journal Article in MOLECULAR ECOLOGY

Polar tube protein gene diversity among Nosema ceranae strains derived from a Greek honey bee health study

Fani HatjinaGeorgios TsoktouridisMaria BougaLeonidas CharistosVassiliki EvangelouDimitrios AvtzisIvan MeeusMarleen BrunainGuy SmaggheDirk de Graaf

A1 Journal Article in JOURNAL OF INVERTEBRATE PATHOLOGY

Updated genome assembly and annotation of Paenibacillus larvae, the agent of American foulbrood disease of honey bees

Queenie WT ChanR Scott CornmanInanc BirolNancy Y LiaoSimon K ChanT Roderick DockingShaun D JackmanGreg A TaylorSteven JM JonesDirk de Graaf et al.

A1 Journal Article in BMC GENOMICS

2010

Functional and Evolutionary Insights from the Genomes of Three Parasitoid Nasonia Species

JH WerrenS RichardsCA DesjardinsO NiehuisJ GadauJK ColbourneLW BeukeboomC DesplanCG ElsikCJP Grimmelikhuijzen et al.

A1 Journal Article in SCIENCE

Honey bee colony losses in Belgium during the 2008-9 winter

Bach Kim NguyenJacques MignonDries LagetDirk de GraafFranciscus JacobsDenis van EngelsdorpYves BrostauxClaude SaegermanEric Haubruge

A1 Journal Article in JOURNAL OF APICULTURAL RESEARCH

Insights into the venom composition of the ectoparasitoid wasp Nasonia vitripennis from bioinformatic and proteomic studies

Dirk de GraafMaarten AertsMarleen BrunainCA DesjardinsFranciscus JacobsJH WerrenBart Devreese

A1 Journal Article in INSECT MOLECULAR BIOLOGY

Multiplex PCR detection of slowly-evolving trypanosomatids and neogregarines in bumblebees using broad-range primers

Ivan MeeusDirk de GraafK JansGuy Smagghe

A1 Journal Article in JOURNAL OF APPLIED MICROBIOLOGY

Multiplex RT-PCR with broad-range primers and an exogenous internal amplification control for the detection of honeybee viruses in bumblebees

Ivan MeeusGuy SmaggheReinhold SiedeKris JansDirk de Graaf

A1 Journal Article in JOURNAL OF INVERTEBRATE PATHOLOGY

Two novel proteins expressed by the venom glands of Apis mellifera and Nasonia vitripennis share an ancient C1q-like domain

Dirk de GraafMarleen BrunainB ScharlakenN PeirenBart DevreeseDG EboWJ StevensCA DesjardinsJH WerrenFranciscus Jacobs

A1 Journal Article in INSECT MOLECULAR BIOLOGY

Venom proteins of the parasitoid wasp Nasonia vitripennis : recent discovery of an untapped pharmacopee

Ellen DanneelsDavid B RiversDirk de Graaf

A1 Journal Article in TOXINS

2009

Bee, wasp and ant venomics pave the way for a component-resolved diagnosis of sting allergy

Dirk de GraafMaarten AertsEllen DanneelsBart Devreese

A1 Journal Article in Journal of Proteomics

Lessons from the first international proficiency test for the detection of spores from the honey bee pathogen Paenibacillus larvae

Dirk de GraafWolfgang RitterFranciscus JacobsMarleen Brunain

A1 Journal Article in ACCREDITATION AND QUALITY ASSURANCE

2008

Differential gene expression in the honeybee head after a bacteria challenge

Bieke ScharlakenDirk de GraafKaren GoossensLuc PeelmanFranciscus Jacobs

A1 Journal Article in DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY

First molecular confirmation of deformed wing virus infections of honeybees from a Belgian apiary reveals the presence of black queen cell virus and Varroa destructor virus 1

Dirk de GraafMarleen BrunainH IMBERECHTSFranciscus Jacobs

A1 Journal Article in VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT

Implementation of quality control and biosafety measurements in the diagnosis of honey bee diseases

Dirk de GraafMarleen BrunainFranciscus Jacobs

A1 Journal Article in JOURNAL OF APICULTURAL RESEARCH

Integrated 'omic' study on the molecular events in the honey bee head after bacterial challenge

Bieke ScharlakenFranciscus JacobsDirk de Graaf

Proteomic analysis of the honey bee worker venom gland focusing on the mechanisms of protection against tissue damage

Nico PeirenDirk de GraafFrank VanrobaeysEllen DanneelsBart DevreeseJozef Van BeeumenFranciscus Jacobs

A1 Journal Article in Toxicon

Reference gene selection for insect expression studies using quantitative real-time PCR: The head of the honeybee, Apis mellifera, after a bacterial challenge

Bieke ScharlakenDirk de GraafKaren GoossensMarleen BrunainLuc PeelmanFranciscus Jacobs

A1 Journal Article in JOURNAL OF INSECT SCIENCE

2007

Differential protein expression in the honey bee head after a bacterial challenge

Bieke ScharlakenDirk de GraafSamy MemmiBart DevreeseJozef Van BeeumenFranciscus Jacobs

A1 Journal Article in ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY

Selection of housekeeping genes for gene expression studies in honey bee head tissue using quantitative real-time PCR

Bieke ScharlakenDirk de GraafKaren GoossensMarleen BrunainLuc PeelmanFranciscus Jacobs

Stability of reference genes for expression studies in the honey bee head after a bacterial challenge using quantitative real-time PCR

Bieke ScharlakenDirk de GraafKaren GoossensMarleen BrunainLuc PeelmanFranciscus Jacobs

2006

Diagnosis of American foulbrood in honey bees: a synthesis and proposed analytical protocols

Dirk de GraafAM ALIPPIM BROWNJD EVANSM FELDLAUFERA GREGORCM HORNITZKYSF PERNALDMT SCHUCHD TITERA et al.

A1 Journal Article in LETTERS IN APPLIED MICROBIOLOGY

Genomic and transcriptional analysis of protein heterogeneity of the honeybee venom allergen Api m 6

Nico PeirenDirk de GraafJD EVANSFranciscus Jacobs

A1 Journal Article in INSECT MOLECULAR BIOLOGY

Identification of Paenibacillus larvae to the subspecies level : an obstacle for AFB diagnosis

Dirk de GraafPaul De VosMarc HeyndrickxStefanie Van TrappenNico PeirenFranciscus Jacobs

A1 Journal Article in JOURNAL OF INVERTEBRATE PATHOLOGY

Insights into social insects from the genome of the honeybee Apis mellifera

G WEINSTOCKG ROBINSONR GIBBSDirk de Graaf

A1 Journal Article in NATURE

Molecular cloning and expression of icarapin, a novel IgE-binding bee venom protein

Nico PeirenDirk de GraafMarleen BrunainCH BRIDTSDG EBOWJ STEVENSFranciscus Jacobs

A1 Journal Article in FEBS LETTERS

Scope for non-wood forest products income generation from rehabilitation areas: focus on beekeeping

Franciscus JacobsCaroline SimoensDirk de GraafJ Deckers

A2 Journal Article in JOURNAL OF THE DRYLANDS

The venom of honeybees (Apis mellifera L.) : from proteomics to recombinant production of potential allergens

Nico PeirenFranciscus JacobsDirk de Graaf

2005

The protein composition of honeybee venom reconsidered by a proteomic approach

Nico PeirenFrank VanrobaeysDirk de GraafBart DevreeseJozef Van BeeumenFranciscus Jacobs

A1 Journal Article in BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

2004

Chapter 2.9.2. American Foulbrood

Bookchapter in OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals

2002

Flow cytometric analysis of lectin-stained haemocytes of the honeybee (Apis mellifera)

Dirk de GraafR DauweK WalravensFranciscus Jacobs

A1 Journal Article in APIDOLOGIE

Screening of the T- and B-cell antigenicity in neonatal calves of the his-tagged Cryptosporidium parvum antigens CP15, CP15/60, P23 and trap-C1

Dirk de GraafH DE CONINCKC DE CLERCQJE PEETERS

A1 Journal Article in FOLIA PARASITOLOGICA

Specific bovine antibody response against a new recombinant Cryptosporidium parvum antigen containing 4 zinc-finger motifs

Dirk de GraafHans De ConinckFranz PetryIlka B EeckhoutJohan E Peeters

A2 Journal Article in KOREAN JOURNAL OF PARASITOLOGY

Varroa destructor-beheersing in de bijenteeltpraktijk

Franciscus JacobsOlivier Van LaereCaroline SimoensDirk de Graaf

2001

Amerikaans Vuilbroed bij Bijen: Optimalisatie van de diagnose en de bestrijding.

W DOBBELAEREDirk de GraafJE PEETERSFranciscus Jacobs

Comparison of two commercial kits for biochemical characterization of Paenibacillus larvae larvae in the diagnosis of AFB.

W DOBBELAEREDirk de GraafJE PEETERSFranciscus Jacobs

A1 Journal Article in JOURNAL OF APICULTURAL RESEARCH

Desinfection of wooden structures contaminated with Paenibacillus larvae subsp. Larvae spores.

W DOBBELAEREDirk de GraafW REYBROECKE DESMEDTJE PEETERSFranciscus Jacobs

A1 Journal Article in JOURNAL OF APPLIED MICROBIOLOGY

Development of a fast and reliable diagnostic method for American foulbrood disease (Paenibacillus larvae subsp larvae) using a 16S rRNA gene based PCR.

W DOBBELAEREDirk de GraafJE PEETERSFranciscus Jacobs

A1 Journal Article in APIDOLOGIE

Influence of the proximity of American foulbrood cases and apicultural management on the prevalence of Paenibacillus larvae spores in Belgian honey.

Dirk de GraafD VANDEKERCHOVEW DOBBELAEREJE PEETERSFranciscus Jacobs

A1 Journal Article in APIDOLOGIE

The possible role of varroa destructor in the transmission of Paenibacillus larvae larvae spores.

JJ JOUBERTPaul De RyckeW DOBBELAEREDirk de GraafH HOSSEINIANFranciscus Jacobs

2000

Varroa jacobsoni as a possible mechanical Vector for Paenibacillus larvae larvae pathogenic to Apis mellifera.

JJ JOUBERTPaul De RyckeW DOBBELAEREDirk de GraafH HOSSEINIANFranciscus Jacobs

1999

A review of the importance of cryptosporidiosis in farm animals

Dirk de GraafE VANOPDENBOSCHLM ORTEGA-MORAH ABBASSIJE PEETERS

A1 Journal Article in INTERNATIONAL JOURNAL FOR PARASITOLOGY

De verspreiding van Amerikaans Vuilbroed in België.

W DOBBELAEREDirk de GraafJE PEETERSFranciscus Jacobs

Paenibacillus larvae, de verwekker van Amerikaans vuilbroed bij de honingbij.

Dirk de GraafW DOBBELAREFranciscus JacobsE PEETERS J

Serological differences in Neospora caninum-associated epidemic and endemic abortions

G SCHARESM RAUSERK ZIMMERM PETERSR WURMJP DUBEYDirk de GraafR EDELHOFERC MERTENSG HESS et al.

A1 Journal Article in JOURNAL OF PARASITOLOGY

Speculation on whether a vaccine against cryptosporidiosis is a reality or fantasy

Dirk de GraafF SPANOF PETRYS SAGODIRAA BONNIN

A1 Journal Article in INTERNATIONAL JOURNAL FOR PARASITOLOGY

1998

A Cryptosporidium parvum oocyst low molecular mass fraction evokes a CD4(+) T-cell-dependent IFN-gamma response in bovine peripheral blood mononuclear cell cultures

Dirk de GraafK WALRAVENSJ GODFROIDJE PEETERS

A1 Journal Article in INTERNATIONAL JOURNAL FOR PARASITOLOGY

1997

Specific interferon-gamma, IgA and IgM responses after experimental infection of neonatal calves with Cryptosporidium parvum

Dirk de GraafJE Peeters

A1 Journal Article in INTERNATIONAL JOURNAL FOR PARASITOLOGY

1996

Complete development of Cryptosporidium parvum in MDBK cells

Isabel VillacortaDirk de GraafGerard CharlierJohan E Peeters

A1 Journal Article in FEMS MICROBIOLOGY LETTERS

Isolation, characterization and immunolocalization of a globin-like antigen from Ostertagia ostertagi adults

Dirk de GraafPaul BerghenLuc MoensTine De MarezStefaan RaesMark L BlaxterJozef Vercruysse

A1 Journal Article in PARASITOLOGY

1995

Cloning and sequencing of an excretory/secretory antigen from Ostertagia ostertagi fourth-stage larvae containing multiple tandem repeats

Dirk de GraafLuc PeelmanEdwin ClaereboutHans HildersonHenk DFH SchalligJozef Vercruysse

A1 Journal Article in MOLECULAR AND BIOCHEMICAL PARASITOLOGY

Immunisation of calves with Ostertagia ostertagi fourth stage larval antigens failed to protect calves from infection

Hans HildersonPaul BerghenDirk de GraafEdwin ClaereboutJozef Vercruysse

A1 Journal Article in INTERNATIONAL JOURNAL FOR PARASITOLOGY

Interactions between Ostertagia ostertagi and Cooperia oncophora in calves

Hans HildersonJozef VercruysseEdwin ClaereboutDirk de GraafJ FransenPaul Berghen

A1 Journal Article in VETERINARY PARASITOLOGY

1994

Early development of Nosema apis (Microspora: Nosematidae) in the midgut epithelium of the honeybee (Apis Mellifera)

Dirk de GraafHilda RaesG SabbeP DeryckeFranciscus Jacobs

A1 Journal Article in JOURNAL OF INVERTEBRATE PATHOLOGY

Identification and isolation of a 19.7 kDa Ostertagia ostertagi specific antigen and evaluation of its potential for immunodiagnosis

Dirk de GraafPaul BerghenHans HildersonEdwin ClaereboutJozef Vercruysse

A1 Journal Article in INTERNATIONAL JOURNAL FOR PARASITOLOGY

Spore dimorphism in Nosema apis (Microsporida, Nosematidae) developmental cycle

Dirk de GraafHilda RaesFranciscus Jacobs

A1 Journal Article in JOURNAL OF INVERTEBRATE PATHOLOGY

1993

Characterisation of Ostertagia ostertagi antigens by the different bovine immunoglobulin isotypes

Hans HildersonDirk de GraafJozef VercruyssePaul Berghen

A1 Journal Article in RESEARCH IN VETERINARY SCIENCE

Cross immunity between Ostertagia ostertagi and Cooperia oncophora in calves

Hans HildersonJozef VercruysseEdwin ClaereboutDirk de GraafJ FransenPaul Berghen

Identification and purification of Cooperia oncophora-specific antigens to improve serological diagnosis

Dirk de GraafPaul BerghenHans HildersonHilde De CockJozef Vercruysse

A1 Journal Article in INTERNATIONAL JOURNAL FOR PARASITOLOGY

In vitro germination of Nosema apis (Microspora: Nosematidae) spores and its effect on their αα-trehalose/D-glucose ratio

Dirk de GraafGeert MasscheleinFrans VandergeynstHubert De BrabanderFranciscus Jacobs

A1 Journal Article in JOURNAL OF INVERTEBRATE PATHOLOGY

Partial characterization of proteolytic enzymes in different developmental stages of Ostertagia ostertagi

Hilde De CockDP KnoxEdwin ClaereboutDirk de Graaf

A1 Journal Article in JOURNAL OF HELMINTHOLOGY

Purification of Cooperia oncophora specific antigens from adult worm extracts and its application in an enzyme linked immunosorbent assay

Dirk de GraafHans HildersonPaul BerghenJozef Vercruysse

The presence of an early L4 larvae population in relation to the immune response of calves against Ostertagia ostertagi

Hans HildersonJozef VercruysseDirk de GraafP BastiaensenJ FransenPaul Berghen

A1 Journal Article in VETERINARY PARASITOLOGY

1992

Antigenic differences between the life cycle stages of Cooperia oncophora

Dirk de GraafHans HildersonPaul BerghenJozef Vercruysse

A1 Journal Article in RESEARCH IN VETERINARY SCIENCE

Partial antigen purification of the cattle nematode Cooperia oncophora

Dirk de GraafPaul BerghenHans HildersonJozef Vercruysse

A1 Journal Article in BELGIAN JOURNAL OF BOTANY

Primaire infectie van de honingbij (Apis mellifera L.) door Nosema apis Zander

Dirk de GraafP.H. De RyckeF.J. Jacobs

1991

Physiological and immunological aspects of the early developing stages of Nosema apis, midgut parasite of the honey bees

Dirk de GraafFranciscus Jacobs

A2 Journal Article in Ann. Soc. belge Méd. trop., nr. 71, p. 159

Tissue specificity of Nosema apis

Dirk de GraafFranciscus Jacobs

A1 Journal Article in JOURNAL OF INVERTEBRATE PATHOLOGY

1988

Hersenproteïnen bij de honingbij (Apis mellifera L.)

L BonnarensDirk de GraafFranciscus Jacobs